Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	980,265	G→C	18.2%	P14A (CCT→GCT) ‡	cspI ←	cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	980,265	0	G	C	18.2%	148.4 / 24.2	66	P14A (CCT→GCT) ‡	cspI	cold shock protein
Reads supporting (aligned to +/- strand):  ref base G (29/25);  new base C (3/9);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

ATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTT  >  minE/980195‑980334                                                                       |                                                                      aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                       >  1:1634379/1‑71 (MQ=255) aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                       >  1:1237321/1‑71 (MQ=255)     cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                                                   <  1:2521743/71‑1 (MQ=255)     cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                                                   <  1:3571031/71‑1 (MQ=255)         gaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                                >  1:3664975/1‑70 (MQ=255)          aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa                                                              >  1:1007396/1‑71 (MQ=255)            atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                                                            <  1:987032/68‑1 (MQ=255)             tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                      >  1:1273661/3‑60 (MQ=255)             tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                      >  1:232045/3‑60 (MQ=255)             tAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                           <  1:2211814/71‑1 (MQ=255)              gcaCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAttt                                                           >  1:2548954/3‑70 (MQ=255)               caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAtttta                                                         <  1:658298/70‑3 (MQ=255)                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                                                        >  1:1534400/1‑70 (MQ=255)                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                                                       <  1:1450160/71‑1 (MQ=255)                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                                                       <  1:2728011/71‑1 (MQ=255)                  aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACTa                                                      <  1:3525091/71‑1 (MQ=255)                   aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                                                     <  1:1430417/71‑1 (MQ=255)                   aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                                                     <  1:3226106/71‑1 (MQ=255)                    acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                      >  1:2235155/1‑53 (MQ=255)                    acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaaa                                                    >  1:3760251/1‑71 (MQ=255)                      acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAcc                                                  <  1:2092435/68‑1 (MQ=9)                      acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                                                   >  1:3505348/4‑70 (MQ=9)                      acaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAcc                                                  <  1:3379269/71‑1 (MQ=255)                              gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                                          >  1:1066236/1‑71 (MQ=255)                              gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                           <  1:2909648/70‑1 (MQ=255)                               gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                         <  1:2536224/69‑1 (MQ=255)                               gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                         <  1:3804793/69‑1 (MQ=255)                                tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                           <  1:2182225/52‑1 (MQ=255)                                tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAt                                                             <  1:531124/50‑1 (MQ=255)                                agcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                         <  1:2864534/69‑1 (MQ=255)                                  ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                                                        <  1:833754/53‑1 (MQ=255)                                  ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                       <  1:2761567/70‑1 (MQ=255)                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaa                                                                  >  1:2866869/1‑42 (MQ=255)                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCa                                                         >  1:400185/1‑51 (MQ=255)                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa                                     <  1:1984849/71‑1 (MQ=255)                                    aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc                                                   <  1:2536476/56‑1 (MQ=255)                                     tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                       <  1:1740353/35‑1 (MQ=255)                                     tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                       <  1:1487346/35‑1 (MQ=255)                                      cTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg                                        >  1:1881533/1‑65 (MQ=255)                                       tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                           >  1:3310296/1‑61 (MQ=255)                                       tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa                                 >  1:1521667/1‑71 (MQ=255)                                          ctgCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATa                               <  1:2200645/68‑1 (MQ=255)                                          cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa                              >  1:3118615/1‑71 (MQ=255)                                          cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa                              >  1:1203435/1‑71 (MQ=255)                                            gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                           <  1:3418694/56‑1 (MQ=255)                                            gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt                            <  1:3671569/71‑1 (MQ=255)                                             cGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat                             <  1:3395416/69‑1 (MQ=255)                                             agTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                                                   >  1:825724/2‑47 (MQ=16)                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                       >  1:3460537/1‑53 (MQ=255)                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCt                     >  1:2399905/1‑71 (MQ=255)                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCt                     >  1:3817724/1‑71 (MQ=255)                                                     aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt                   >  1:3280759/1‑71 (MQ=255)                                                       ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg                 <  1:2936467/71‑1 (MQ=255)                                                       ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg                 <  1:800234/71‑1 (MQ=255)                                                       ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCAtttt                                         <  1:1918042/47‑1 (MQ=255)                                                         aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATGATTACCTTTTg                 >  1:1369223/1‑69 (MQ=255)                                                          aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                                          >  1:590728/1‑43 (MQ=255)                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga             >  1:3047348/1‑70 (MQ=255)                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga             >  1:769702/1‑70 (MQ=255)                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaag              >  1:2201983/1‑69 (MQ=255)                                                              cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt          <  1:2112271/71‑1 (MQ=255)                                                              cTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                         <  1:2318751/40‑1 (MQ=16)                                                                ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg                 >  1:3350166/1‑62 (MQ=255)                                                                ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt          >  1:3311983/1‑69 (MQ=255)                                                                 tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                       >  1:3499314/1‑39 (MQ=255)                                                                 tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat                          >  1:2159221/1‑52 (MQ=255)                                                                   tCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGACAtt                               <  1:3601552/45‑2 (MQ=255)                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc    >  1:1412852/1‑71 (MQ=255)                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc    >  1:1707663/1‑71 (MQ=255)                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGcc     <  1:349227/70‑1 (MQ=255)                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa                                     >  1:554007/1‑37 (MQ=255)                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt         >  1:3329426/1‑65 (MQ=255)                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc    <  1:222247/70‑1 (MQ=255)                                                                      gggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCtt  <  1:541404/71‑1 (MQ=255)                                                                      gggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCtt  <  1:726070/71‑1 (MQ=255)                                                                      gggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCtt  <  1:3305656/71‑1 (MQ=255)                                                                       |                                                                      ATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTT  >  minE/980195‑980334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑