Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 982,629 C→T 29.6% T25T (ACC→ACT)  ydfA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE982,6290CT29.6% 140.3 / 73.6 98T25T (ACC→ACT) ydfAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/38);  new base T (12/17);  total (43/55)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

Only 100 of 104 total aligned reads displayed.

ATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACT  >  minE/982559‑982699
                                                                      |                                                                      
aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAcc                                                                        <  1:2350193/71‑1 (MQ=255)
  aaaaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGc                                                                      >  1:3303811/4‑71 (MQ=255)
  aaaaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGc                                                                      >  1:3427573/4‑71 (MQ=255)
  aaaaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGc                                                                      >  1:3550938/4‑71 (MQ=255)
  aCTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                                                       >  1:3766279/1‑70 (MQ=255)
  aCTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                                                       >  1:398446/1‑70 (MQ=255)
   cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                                                      <  1:743707/70‑1 (MQ=255)
   cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                                                     <  1:1231933/71‑1 (MQ=255)
      tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                                                     <  1:2412675/68‑1 (MQ=255)
      tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                                                                   >  1:3322947/1‑70 (MQ=255)
       cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                                                                  <  1:52514/70‑1 (MQ=255)
        gATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                                                                    >  1:2157967/1‑67 (MQ=255)
        gATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGAc                                                                >  1:3867343/1‑71 (MQ=255)
         aTCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                                               >  1:2787413/1‑71 (MQ=255)
           ctcggCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGACATGACGta                                                             >  1:1891871/4‑71 (MQ=255)
           cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                                                             >  1:2786558/1‑71 (MQ=255)
              ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                                               <  1:1468420/66‑1 (MQ=255)
               gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATAcc                                                         <  1:1824892/71‑1 (MQ=255)
                 aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                                                       <  1:293877/71‑1 (MQ=255)
                 aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                                                       <  1:1500612/71‑1 (MQ=255)
                  acaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                                                      <  1:2874776/55‑1 (MQ=255)
                  acaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                                                       >  1:226656/1‑70 (MQ=255)
                  acaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGACGGAACGTTCACTGCCATGACGTATACCaaa                                                      <  1:1574190/71‑1 (MQ=255)
                   caacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                                                       >  1:2300351/1‑69 (MQ=255)
                    aacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                                                       >  1:2506737/1‑52 (MQ=255)
                     acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                                                                  <  1:3672106/56‑1 (MQ=255)
                     acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGc                                                   <  1:2943475/71‑1 (MQ=255)
                     acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAAGGCACGTTCACCGCAATGACGTATACCAAAAGc                                                   <  1:499196/71‑1 (MQ=255)
                     acGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   <  1:2543305/71‑1 (MQ=255)
                      cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCGCTGCCATGACGTATACCAAAAGCa                                                  <  1:744226/71‑1 (MQ=255)
                      cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCa                                                        >  1:2377970/1‑65 (MQ=255)
                      cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAg                                                    >  1:2063079/1‑69 (MQ=255)
                      cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   >  1:667690/1‑70 (MQ=255)
                      cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCa                                                  <  1:296415/71‑1 (MQ=255)
                       gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                                                 <  1:3690503/71‑1 (MQ=255)
                       gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  >  1:3536988/1‑70 (MQ=255)
                       gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  >  1:3532453/1‑70 (MQ=255)
                         aTCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   <  1:39852/67‑1 (MQ=255)
                            tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGt                                            >  1:1360865/1‑71 (MQ=255)
                             ctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAtt                                           >  1:1404636/1‑71 (MQ=255)
                             ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaaa                                               >  1:816828/1‑67 (MQ=255)
                             ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                                                >  1:2360745/1‑66 (MQ=255)
                              tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                           >  1:386150/1‑70 (MQ=255)
                              tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                           >  1:311942/1‑70 (MQ=255)
                                gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                        <  1:2970778/71‑1 (MQ=255)
                                gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:238927/71‑1 (MQ=255)
                                gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3844028/71‑1 (MQ=255)
                                   tACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  <  1:176364/58‑1 (MQ=255)
                                     cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                         >  1:3916346/1‑65 (MQ=255)
                                     cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAAcc                                    <  1:3676827/70‑1 (MQ=255)
                                     cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                        <  1:2735242/66‑1 (MQ=255)
                                      ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa                                  <  1:41107/71‑1 (MQ=255)
                                       gCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGa                                  >  1:2761011/1‑70 (MQ=255)
                                       gCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt                                          <  1:1738321/62‑1 (MQ=255)
                                        cGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaa                                       <  1:23565/64‑1 (MQ=255)
                                         gtgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               <  1:553774/71‑1 (MQ=255)
                                          tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa                                      >  1:1087032/1‑63 (MQ=255)
                                            tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               <  1:1761545/68‑1 (MQ=255)
                                            tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                            <  1:3158471/71‑1 (MQ=255)
                                            tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                            <  1:851923/71‑1 (MQ=255)
                                              tCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTg                          >  1:2929104/1‑71 (MQ=255)
                                               cccaaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                         >  1:1490063/4‑71 (MQ=255)
                                               ccAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        >  1:2051826/1‑56 (MQ=255)
                                                cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc                         <  1:3745069/70‑1 (MQ=255)
                                                 caaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa                                >  1:3023933/2‑62 (MQ=255)
                                                     cAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTg                             <  1:3667758/61‑1 (MQ=255)
                                                      aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCg                   <  1:784787/70‑1 (MQ=255)
                                                      aGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgc                  <  1:52041/71‑1 (MQ=255)
                                                       ggATGGAACGTTCACTGGCATGACGTATACCAAAAGCAAAACATTTaaaa                                      >  1:2531996/1‑50 (MQ=255)
                                                        gACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                         <  1:572479/46‑1 (MQ=255)
                                                         aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGttt                                          <  1:671834/44‑1 (MQ=255)
                                                         aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctgg               <  1:2816653/71‑1 (MQ=255)
                                                            gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                 <  1:1522643/50‑1 (MQ=255)
                                                            gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctggctg            >  1:1633841/1‑71 (MQ=255)
                                                             aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg           <  1:2702312/70‑1 (MQ=255)
                                                             aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg           <  1:3140303/70‑1 (MQ=255)
                                                              aCGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:570782/71‑1 (MQ=255)
                                                               cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                         >  1:1324791/1‑55 (MQ=255)
                                                               cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag         <  1:1362761/71‑2 (MQ=255)
                                                               cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa         <  1:2415209/71‑1 (MQ=255)
                                                               cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          >  1:2869985/1‑70 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3501450/39‑1 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:707003/39‑1 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3811901/39‑1 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa        <  1:2181387/71‑1 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa        <  1:2718327/71‑1 (MQ=255)
                                                                gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:1293730/69‑1 (MQ=255)
                                                                 ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                 <  1:3314056/45‑1 (MQ=255)
                                                                 ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag       <  1:519500/71‑1 (MQ=255)
                                                                  tCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag       >  1:777994/1‑70 (MQ=255)
                                                                   cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               >  1:27844/1‑45 (MQ=255)
                                                                   cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:2005320/1‑70 (MQ=255)
                                                                   cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      <  1:2544152/70‑1 (MQ=255)
                                                                   cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:2709370/1‑70 (MQ=255)
                                                                   cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:3461625/1‑70 (MQ=255)
                                                                   cACCGCAATGACGTATACAAAAAGCAAAACGTTTAAAAc                                     >  1:2901478/1‑39 (MQ=255)
                                                                     ccGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:2051769/64‑1 (MQ=255)
                                                                      cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg                                   <  1:2518115/38‑1 (MQ=255)
                                                                      cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt  >  1:385486/1‑71 (MQ=255)
                                                                      cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt  >  1:860743/1‑71 (MQ=255)
                                                                      |                                                                      
ATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACT  >  minE/982559‑982699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑