Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	982,632	A→C	28.3%	A26A (GCA→GCC)	ydfA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	982,632	0	A	C	28.3%	92.1 / 76.3	92	A26A (GCA→GCC)	ydfA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/36);  new base C (9/17);  total (39/53)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACT  >  minE/982562‑982699                                                                       |                                                                    cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                                                     <  1:1231933/71‑1 (MQ=255)    tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                                                     <  1:2412675/68‑1 (MQ=255)    tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                                                                   >  1:3322947/1‑70 (MQ=255)     cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                                                                  <  1:52514/70‑1 (MQ=255)      gATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                                                                    >  1:2157967/1‑67 (MQ=255)      gATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGAc                                                                >  1:3867343/1‑71 (MQ=255)       aTCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                                               >  1:2787413/1‑71 (MQ=255)         ctcggCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGACATGACGta                                                             >  1:1891871/4‑71 (MQ=255)         cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                                                             >  1:2786558/1‑71 (MQ=255)            ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                                               <  1:1468420/66‑1 (MQ=255)             gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATAcc                                                         <  1:1824892/71‑1 (MQ=255)               aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                                                       <  1:1500612/71‑1 (MQ=255)               aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                                                       <  1:293877/71‑1 (MQ=255)                acaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                                                       >  1:226656/1‑70 (MQ=255)                acaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGACGGAACGTTCACTGCCATGACGTATACCaaa                                                      <  1:1574190/71‑1 (MQ=255)                 caacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                                                       >  1:2300351/1‑69 (MQ=255)                   acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                                                                  <  1:3672106/56‑1 (MQ=255)                   acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGc                                                   <  1:2943475/71‑1 (MQ=255)                   acGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAAGGCACGTTCACCGCAATGACGTATACCAAAAGc                                                   <  1:499196/71‑1 (MQ=255)                   acGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   <  1:2543305/71‑1 (MQ=255)                    cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCGCTGCCATGACGTATACCAAAAGCa                                                  <  1:744226/71‑1 (MQ=255)                    cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCa                                                        >  1:2377970/1‑65 (MQ=255)                    cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAg                                                    >  1:2063079/1‑69 (MQ=255)                    cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   >  1:667690/1‑70 (MQ=255)                    cGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCa                                                  <  1:296415/71‑1 (MQ=255)                     gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                                                 <  1:3690503/71‑1 (MQ=255)                     gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  >  1:3532453/1‑70 (MQ=255)                     gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  >  1:3536988/1‑70 (MQ=255)                       aTCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGc                                                   <  1:39852/67‑1 (MQ=255)                          tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGt                                            >  1:1360865/1‑71 (MQ=255)                           ctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAtt                                           >  1:1404636/1‑71 (MQ=255)                           ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaaa                                               >  1:816828/1‑67 (MQ=255)                           ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                                                >  1:2360745/1‑66 (MQ=255)                            tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                           >  1:311942/1‑70 (MQ=255)                            tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                           >  1:386150/1‑70 (MQ=255)                              gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                        <  1:2970778/71‑1 (MQ=255)                              gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3844028/71‑1 (MQ=255)                              gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:238927/71‑1 (MQ=255)                                 tACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCa                                                  <  1:176364/58‑1 (MQ=255)                                   cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                         >  1:3916346/1‑65 (MQ=255)                                   cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAAcc                                    <  1:3676827/70‑1 (MQ=255)                                   cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                        <  1:2735242/66‑1 (MQ=255)                                    ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa                                  <  1:41107/71‑1 (MQ=255)                                     gCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGa                                  >  1:2761011/1‑70 (MQ=255)                                     gCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt                                          <  1:1738321/62‑1 (MQ=255)                                      cGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaa                                       <  1:23565/64‑1 (MQ=255)                                       gtgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               <  1:553774/71‑1 (MQ=255)                                        tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa                                      >  1:1087032/1‑63 (MQ=255)                                        tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa                                      >  1:2926376/1‑63 (MQ=255)                                          tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               <  1:1761545/68‑1 (MQ=255)                                          tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                            <  1:3158471/71‑1 (MQ=255)                                          tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                            <  1:851923/71‑1 (MQ=255)                                            tCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTg                          >  1:2929104/1‑71 (MQ=255)                                             cccaaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                         >  1:1490063/4‑71 (MQ=255)                                             ccAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        >  1:2051826/1‑56 (MQ=255)                                              cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc                         <  1:3745069/70‑1 (MQ=255)                                               caaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa                                >  1:3023933/2‑62 (MQ=255)                                                   cAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTg                             <  1:3667758/61‑1 (MQ=255)                                                    aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCg                   <  1:784787/70‑1 (MQ=255)                                                    aGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgc                  <  1:52041/71‑1 (MQ=255)                                                     ggATGGAACGTTCACTGGCATGACGTATACCAAAAGCAAAACATTTaaaa                                      >  1:2531996/1‑50 (MQ=255)                                                      gACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                         <  1:572479/46‑1 (MQ=255)                                                       aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGttt                                          <  1:671834/44‑1 (MQ=255)                                                       aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctgg               <  1:2816653/71‑1 (MQ=255)                                                          gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                 <  1:1522643/50‑1 (MQ=255)                                                          gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctggctg            >  1:1633841/1‑71 (MQ=255)                                                           aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg            <  1:3835257/69‑1 (MQ=255)                                                           aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg           <  1:3140303/70‑1 (MQ=255)                                                           aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg           <  1:2702312/70‑1 (MQ=255)                                                            aCGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:570782/71‑1 (MQ=255)                                                             cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                         >  1:1324791/1‑55 (MQ=255)                                                             cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag         <  1:1362761/71‑2 (MQ=255)                                                             cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa         <  1:2415209/71‑1 (MQ=255)                                                             cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          >  1:2869985/1‑70 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:707003/39‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3501450/39‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:3811901/39‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                        <  1:2297997/39‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa        <  1:2718327/71‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa        <  1:2181387/71‑1 (MQ=255)                                                              gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:1293730/69‑1 (MQ=255)                                                               ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                 <  1:3314056/45‑1 (MQ=255)                                                               ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag       <  1:519500/71‑1 (MQ=255)                                                                tCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag       >  1:777994/1‑70 (MQ=255)                                                                 cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                               >  1:27844/1‑45 (MQ=255)                                                                 cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:3461625/1‑70 (MQ=255)                                                                 cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:2005320/1‑70 (MQ=255)                                                                 cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      <  1:2544152/70‑1 (MQ=255)                                                                 cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga      >  1:2709370/1‑70 (MQ=255)                                                                 cACCGCAATGACGTATACAAAAAGCAAAACGTTTAAAAc                                     >  1:2901478/1‑39 (MQ=255)                                                                   ccGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga          <  1:2051769/64‑1 (MQ=255)                                                                    cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg                                   <  1:2518115/38‑1 (MQ=255)                                                                    cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt  >  1:385486/1‑71 (MQ=255)                                                                    cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt  >  1:860743/1‑71 (MQ=255)                                                                      cAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc                    >  1:54342/1‑51 (MQ=255)                                                                       |                                                                    CTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACT  >  minE/982562‑982699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑