Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	982,656	G→A	23.0%	T34T (ACG→ACA)	ydfA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	982,656	0	G	A	23.0%	167.4 / 46.0	87	T34T (ACG→ACA)	ydfA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base A (6/14);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

TCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACAC  >  minE/982587‑982725                                                                      |                                                                      tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGt                                                                      >  1:1360865/1‑71 (MQ=255)  ctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAtt                                                                     >  1:1404636/1‑71 (MQ=255)   tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                                                     >  1:311942/1‑70 (MQ=255)   tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGtt                                                                     >  1:386150/1‑70 (MQ=255)     gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                                                  <  1:2970778/71‑1 (MQ=255)     gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:238927/71‑1 (MQ=255)     gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:3844028/71‑1 (MQ=255)          cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                                                   >  1:3916346/1‑65 (MQ=255)          cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAAcc                                                              <  1:3676827/70‑1 (MQ=255)          cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa                                                                  <  1:2735242/66‑1 (MQ=255)           ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa                                                            <  1:41107/71‑1 (MQ=255)            gCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGa                                                            >  1:2761011/1‑70 (MQ=255)            gCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt                                                                    <  1:1738321/62‑1 (MQ=255)             cGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaa                                                                 <  1:23565/64‑1 (MQ=255)              gtgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                                                         <  1:553774/71‑1 (MQ=255)               tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa                                                                >  1:2926376/1‑63 (MQ=255)               tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa                                                                >  1:1087032/1‑63 (MQ=255)                 tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                                                         <  1:1761545/68‑1 (MQ=255)                 tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                                                      <  1:851923/71‑1 (MQ=255)                 tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg                                                      <  1:3158471/71‑1 (MQ=255)                   tCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTg                                                    >  1:2929104/1‑71 (MQ=255)                    cccaaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                                                   >  1:1490063/4‑71 (MQ=255)                    ccAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  >  1:2051826/1‑56 (MQ=255)                     cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc                                                   <  1:3745069/70‑1 (MQ=255)                      caaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa                                                          >  1:3023933/2‑62 (MQ=255)                          cAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTg                                                       <  1:3667758/61‑1 (MQ=255)                           aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCg                                             <  1:784787/70‑1 (MQ=255)                           aGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgc                                            <  1:52041/71‑1 (MQ=255)                            ggATGGAACGTTCACTGGCATGACGTATACCAAAAGCAAAACATTTaaaa                                                                >  1:2531996/1‑50 (MQ=255)                             gACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa                                                                   <  1:572479/46‑1 (MQ=255)                              aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGttt                                                                    <  1:671834/44‑1 (MQ=255)                              aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctgg                                         <  1:2816653/71‑1 (MQ=255)                                 gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                                           <  1:1522643/50‑1 (MQ=255)                                 gCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctggctg                                      >  1:1633841/1‑71 (MQ=255)                                  aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg                                      <  1:3835257/69‑1 (MQ=255)                                  aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg                                     <  1:3140303/70‑1 (MQ=255)                                  aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg                                     <  1:2702312/70‑1 (MQ=255)                                   aCGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga                                    <  1:570782/71‑1 (MQ=255)                                    cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                                                   >  1:1324791/1‑55 (MQ=255)                                    cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag                                   <  1:1362761/71‑2 (MQ=255)                                    cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa                                   <  1:2415209/71‑1 (MQ=255)                                    cGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga                                    >  1:2869985/1‑70 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:707003/39‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:2297997/39‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:3811901/39‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa                                                                  <  1:3501450/39‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa                                  <  1:2718327/71‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa                                  <  1:2181387/71‑1 (MQ=255)                                     gTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga                                    <  1:1293730/69‑1 (MQ=255)                                      ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa                                                           <  1:3314056/45‑1 (MQ=255)                                      ttCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag                                 <  1:519500/71‑1 (MQ=255)                                       tCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaag                                 >  1:777994/1‑70 (MQ=255)                                        cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa                                                         >  1:27844/1‑45 (MQ=255)                                        cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga                                >  1:3461625/1‑70 (MQ=255)                                        cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga                                >  1:2709370/1‑70 (MQ=255)                                        cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga                                <  1:2544152/70‑1 (MQ=255)                                        cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga                                >  1:2005320/1‑70 (MQ=255)                                        cACCGCAATGACGTATACAAAAAGCAAAACGTTTAAAAc                                                               >  1:2901478/1‑39 (MQ=255)                                          ccGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga                                    <  1:2051769/64‑1 (MQ=255)                                           cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg                                                             <  1:2518115/38‑1 (MQ=255)                                           cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt                            >  1:860743/1‑71 (MQ=255)                                           cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt                            >  1:385486/1‑71 (MQ=255)                                             cAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc                                              >  1:54342/1‑51 (MQ=255)                                                 gACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGc                                                   >  1:2420514/1‑42 (MQ=38)                                                     tataCCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggtt                                       <  1:2769810/50‑3 (MQ=255)                                                     tataCCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag                                   <  1:3588852/54‑2 (MQ=255)                                                      ataCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa                              >  1:2049951/1‑58 (MQ=255)                                                       taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgagtga                 <  1:2102060/70‑1 (MQ=255)                                                        aCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgct                                           <  1:2539005/43‑1 (MQ=255)                                                          cAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATg             <  1:3491763/71‑1 (MQ=255)                                                           aaaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtga                     >  1:3530296/1‑62 (MQ=255)                                                           aaaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATgg            <  1:815703/71‑1 (MQ=255)                                                            aaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGa           >  1:880873/1‑71 (MQ=255)                                                            aaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGa           >  1:2436610/1‑71 (MQ=255)                                                            aaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGa           >  1:1128136/1‑71 (MQ=255)                                                             aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgct                                           >  1:1036833/1‑38 (MQ=255)                                                             aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGAt          <  1:543831/71‑1 (MQ=255)                                                                  aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa                              >  1:56866/1‑46 (MQ=255)                                                                  aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt                       <  1:1946963/53‑1 (MQ=255)                                                                  aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGa     <  1:1398796/71‑1 (MQ=255)                                                                  aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCACGTGAGTGATATGGATTTCGa     >  1:113236/1‑71 (MQ=255)                                                                  aaaCATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag                                   <  1:3593249/41‑2 (MQ=12)                                                                  aaaCATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag                                   <  1:2567329/41‑2 (MQ=12)                                                                   aaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGa     >  1:252705/1‑70 (MQ=255)                                                                    aCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa                              >  1:3720713/1‑44 (MQ=255)                                                                    aCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAgg                        >  1:977316/1‑50 (MQ=255)                                                                     cGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGacac  <  1:1396034/71‑1 (MQ=255)                                                                      |                                                                      TCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACAC  >  minE/982587‑982725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑