| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,959 | A→G | 14.1% | intergenic (‑51/‑55) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,959 | 0 | A | G | 14.1% | 263.5 / 29.8 | 106 | intergenic (‑51/‑55) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (37/54); new base G (8/7); total (45/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
AGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGA > minE/217889‑218027 | aGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCa < 1:1364410/71‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATc < 1:1384266/67‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:498420/70‑1 (MQ=255) ctctGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1755118/69‑1 (MQ=255) tGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:717035/61‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:2888069/69‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2663366/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:3761061/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:424125/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACt > 1:724093/1‑71 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:2077113/71‑1 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:1390659/71‑1 (MQ=255) cATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:245371/66‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGt < 1:2888312/70‑1 (MQ=255) taaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg < 1:2942122/71‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:2464969/52‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:1686334/4‑71 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2987154/68‑1 (MQ=255) ggTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa < 1:550551/65‑1 (MQ=255) agTTCTGCTGATTTAACCGCTGAGAAATTCGCCAGAGCGATCCGAAATCACTGGCACg < 1:3873982/57‑1 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAtgtg > 1:1451689/2‑60 (MQ=255) ttCTGCTGATTTAACCGCAGAGAATTTCGCCACAGCaa < 1:778613/38‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1956657/43‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAAc > 1:2779023/1‑46 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGc < 1:3013101/71‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:3736631/52‑1 (MQ=255) tCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:2583378/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:3199143/41‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:1189466/1‑71 (MQ=255) gctgTTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGc < 1:2825032/49‑1 (MQ=255) ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:918029/70‑3 (MQ=255) ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:2072619/1‑71 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:2612697/70‑3 (MQ=255) tgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc > 1:2602757/1‑71 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa > 1:920853/1‑42 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgaga < 1:1378176/55‑1 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:410811/68‑1 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCTGAAACCACTGGCATGTGGAGAATAAGCTg > 1:464516/1‑61 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTc < 1:3655820/71‑1 (MQ=255) ttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:964310/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGga < 1:2104179/49‑1 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3661896/1‑70 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3740812/1‑70 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAc > 1:2507046/1‑42 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa < 1:1274479/71‑1 (MQ=255) aCCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg > 1:2422969/1‑46 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:2536068/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:1126576/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:2699247/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGga > 1:1471115/1‑46 (MQ=255) ccGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:906744/71‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:3368884/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt > 1:2266602/3‑47 (MQ=37) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTgg < 1:1674402/41‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg > 1:473284/1‑71 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:1954758/38‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:2096358/1‑54 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1641344/68‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:2536499/1‑69 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:294466/48‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:2232146/48‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:1028133/3‑70 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:570190/3‑70 (MQ=255) aattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1675544/69‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1511678/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:3521627/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:2857703/71‑1 (MQ=255) gTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:124471/1‑70 (MQ=39) gTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg > 1:2895760/1‑70 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:3447269/69‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:3804863/1‑61 (MQ=38) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:752732/1‑70 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg > 1:1765062/1‑48 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:3808671/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:1816668/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAACCTGCACAGGCGTCTGGACGTGGTaatgaat > 1:3490900/1‑70 (MQ=255) gCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:1425715/1‑70 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga < 1:522549/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:607213/61‑1 (MQ=38) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2608476/61‑1 (MQ=38) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3806731/69‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat < 1:618958/68‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:3257089/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:3904199/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:1179097/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa > 1:876085/1‑54 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGCAGTGGTAATGAATGAAga > 1:3267468/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2976693/59‑1 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:281643/1‑44 (MQ=38) caGCAATCGGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:392748/1‑71 (MQ=255) caGCAATCCGAAACCCCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1126792/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:252004/53‑1 (MQ=38) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:794802/1‑71 (MQ=39) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:337863/1‑65 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:1757602/60‑1 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:329316/60‑1 (MQ=255) aGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1256877/1‑70 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:569411/70‑1 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:341886/70‑1 (MQ=255) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTa > 1:945283/1‑56 (MQ=255) | AGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGA > minE/217889‑218027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |