| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 983,519 | G→A | 14.9% | intergenic (+431/‑137) | ydfC → / → dicB | hypothetical protein/cell division inhibition protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 983,519 | 0 | G | A | 14.9% | 115.3 / 11.2 | 47 | intergenic (+431/‑137) | ydfC/dicB | hypothetical protein/cell division inhibition protein |
| Reads supporting (aligned to +/- strand): ref base G (22/18); new base A (2/5); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
CAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGT > minE/983452‑983588 | cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:2105614/1‑71 (MQ=255) tttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAc > 1:2003373/1‑71 (MQ=255) ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg > 1:1446336/1‑71 (MQ=255) ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg > 1:3508298/1‑71 (MQ=255) ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAc < 1:1242156/70‑1 (MQ=255) tACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:1626949/70‑1 (MQ=255) tCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg > 1:703380/1‑70 (MQ=255) cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:1744422/1‑70 (MQ=255) tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGATTGCGGCTGTGCGCACGCGGAACa < 1:3273197/70‑1 (MQ=255) cTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGGACGCGGAACAGTTa < 1:1872369/71‑1 (MQ=255) ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa > 1:2045059/1‑71 (MQ=255) ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:1253196/69‑1 (MQ=255) ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:3112833/69‑1 (MQ=255) gccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCa > 1:2303951/1‑70 (MQ=255) cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcgc < 1:3592838/50‑1 (MQ=255) ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3708794/1‑69 (MQ=255) ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:2745282/1‑69 (MQ=255) cTTATTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:2741076/59‑1 (MQ=255) tttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa < 1:2502227/60‑1 (MQ=255) tttttAAAGTGAATTTTGTCATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa < 1:1748936/60‑1 (MQ=255) ttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaagt < 1:447940/61‑3 (MQ=255) tttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGAGCACGCgg > 1:3257778/1‑47 (MQ=255) tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTggc > 1:800831/1‑47 (MQ=37) tAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGt < 1:113572/51‑1 (MQ=37) aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:368105/70‑1 (MQ=255) aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:608316/70‑1 (MQ=255) aaGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTa > 1:1951489/1‑51 (MQ=37) tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:991038/40‑1 (MQ=255) tGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAg < 1:2499119/45‑1 (MQ=25) aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATgg < 1:77106/67‑1 (MQ=255) aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg < 1:2978353/70‑1 (MQ=255) ttttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAt > 1:3282629/1‑71 (MQ=255) ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTa > 1:459098/1‑60 (MQ=255) gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtctc > 1:1177374/1‑71 (MQ=255) tgATGTGGTGAATGCGGCTGAGCGCACGCGGAACa > 1:2955667/1‑35 (MQ=255) gATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg > 1:1738694/1‑62 (MQ=255) tgtgGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTa < 1:1642272/71‑1 (MQ=255) tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAt < 1:2617353/70‑1 (MQ=255) tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAt < 1:2612737/70‑1 (MQ=255) gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTgg > 1:1505269/1‑57 (MQ=255) gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTg > 1:201841/1‑65 (MQ=255) gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCg > 1:545256/1‑71 (MQ=255) tgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCgg < 1:1623228/71‑1 (MQ=255) gAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc > 1:3534131/1‑41 (MQ=255) gAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGt > 1:1533084/1‑53 (MQ=255) aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACAAAAAACAGTGTTATGGGTGGAGTctct > 1:483760/1‑61 (MQ=255) cTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGt < 1:2666997/45‑1 (MQ=255) gAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGt > 1:3049490/1‑70 (MQ=255) | CAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGT > minE/983452‑983588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |