| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,009,234 | G→A | 15.1% | D190N (GAT→AAT) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,009,234 | 0 | G | A | 15.1% | 128.4 / 13.8 | 53 | D190N (GAT→AAT) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (21/24); new base A (5/3); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
CCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > minE/1009167‑1009300 | ccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataat > 1:953325/1‑70 (MQ=255) cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:1246864/1‑71 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACaagatga > 1:3446357/1‑70 (MQ=255) cAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCACCGCTGATCCTGTTTAATGAACatgaagatg > 1:2007666/1‑71 (MQ=255) gCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatga > 1:1261322/1‑63 (MQ=255) gCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCGGAata > 1:2828176/1‑71 (MQ=255) gCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAat < 1:3920685/70‑1 (MQ=255) gCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAat < 1:3494720/70‑1 (MQ=255) aCCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATc > 1:1448886/1‑71 (MQ=255) ccGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATc > 1:3151163/1‑70 (MQ=255) ggAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGac < 1:665425/71‑1 (MQ=255) gAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGac > 1:2325116/1‑70 (MQ=255) aagtgaaagtgaATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCg > 1:1766628/1‑67 (MQ=255) aagtgaaagtgaATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGacaa > 1:3338436/1‑71 (MQ=255) agtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:38784/53‑1 (MQ=255) agtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:1775461/53‑1 (MQ=255) agtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGacaa > 1:379600/1‑70 (MQ=255) aaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgc < 1:1304892/71‑1 (MQ=255) aaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGgc > 1:3606797/1‑71 (MQ=255) aagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgc < 1:1030429/70‑1 (MQ=255) tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCt > 1:1713574/1‑70 (MQ=255) gaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTg > 1:376103/1‑70 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:2466619/43‑1 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat > 1:3309896/1‑42 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat > 1:2817511/1‑42 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat > 1:3639789/1‑42 (MQ=255) ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgatg > 1:3711260/1‑44 (MQ=255) tCTATTGCGCCATCGCTGATCCTGTTTAATGAACatg < 1:222865/37‑1 (MQ=255) cTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:1345182/1‑40 (MQ=255) gcgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAgg > 1:2224566/1‑55 (MQ=255) cgcCATCGCTTATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:1609946/71‑1 (MQ=255) cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:2657536/71‑1 (MQ=255) cgcCATCGCTGACCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACt < 1:3301220/70‑1 (MQ=255) gcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCt < 1:2310221/57‑1 (MQ=255) gcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa < 1:1060280/62‑1 (MQ=255) gcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa < 1:819115/62‑1 (MQ=255) ccATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgc > 1:1392980/1‑53 (MQ=255) cATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatg < 1:66728/71‑1 (MQ=255) cATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatg < 1:3508169/71‑1 (MQ=255) cATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatg < 1:1026550/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:1525039/71‑1 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa > 1:2044311/1‑71 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa > 1:2724608/1‑71 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:2887736/70‑1 (MQ=255) cTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAAt < 1:3524336/58‑1 (MQ=255) tGATCCTGTTTAATGAACATAATGATGCCGAATATCGaca < 1:3109632/40‑1 (MQ=255) tGATCCTGTTTAATGAACATAATGATGCCGAATATCGaca < 1:1121874/40‑1 (MQ=255) tGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAAcc > 1:1472451/1‑71 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCa < 1:3513810/57‑1 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACcgc > 1:3001943/1‑71 (MQ=255) aTCCTGTTTAATGAACATAATGATGCCGAATATCGacaac > 1:2992844/1‑40 (MQ=255) cTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:53733/57‑1 (MQ=255) gTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTg > 1:1981287/1‑71 (MQ=255) tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTg < 1:853823/70‑1 (MQ=255) ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgat > 1:3833998/1‑55 (MQ=255) tAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtga > 1:1134204/1‑53 (MQ=255) tAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg > 1:2462110/1‑52 (MQ=255) aaTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACcgc < 1:2592287/62‑1 (MQ=255) aaTGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGc > 1:1926109/1‑69 (MQ=255) gAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga > 1:2676728/1‑70 (MQ=255) gAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga > 1:824279/1‑70 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTg < 1:1688726/35‑1 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTg < 1:554959/35‑1 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaa < 1:411295/71‑1 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGc > 1:114930/1‑64 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga > 1:274006/1‑68 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:863792/71‑1 (MQ=255) tgatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGcc > 1:3460750/1‑57 (MQ=255) tgatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGcc > 1:2818314/1‑57 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga > 1:1887244/1‑46 (MQ=255) | CCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > minE/1009167‑1009300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |