Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,014,491 A→C 26.7% L526L (CTT→CTG fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,014,4910AC26.7% 29.2 / 20.9 30L526L (CTT→CTGfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base A (9/13);  new base C (3/5);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

TATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAA  >  minE/1014423‑1014561
                                                                    |                                                                      
tATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAg                                                                      >  1:802756/1‑71 (MQ=255)
   ttCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACaa                                                                       <  1:574330/67‑1 (MQ=255)
     cacacaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa                                                                  <  1:1409133/70‑1 (MQ=255)
       cacaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAAc                                                               <  1:2397066/71‑1 (MQ=255)
        acaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACg                                                              >  1:766524/1‑71 (MQ=255)
                   aTTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCt                                                   <  1:879644/71‑1 (MQ=255)
                        gtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTcc                                                <  1:3225121/69‑1 (MQ=255)
                          gAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACg                                                              >  1:520733/1‑53 (MQ=255)
                          gAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACg                                                              >  1:3318998/1‑53 (MQ=255)
                               gTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAt                                        <  1:786651/70‑1 (MQ=255)
                                tATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAttt                                      <  1:3271043/71‑1 (MQ=255)
                                     gctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCa                                  <  1:1875849/70‑1 (MQ=255)
                                     gctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGaa                                                       <  1:1684018/49‑1 (MQ=255)
                                      ctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCCGGAAATCTTCCACTTCAATTTTCCAg                                 <  1:1928436/70‑1 (MQ=255)
                                         gaagaaGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGAt                               <  1:1659224/69‑1 (MQ=255)
                                          aagaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGc                            <  1:2995623/71‑1 (MQ=255)
                                           agaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCt                           <  1:1022793/71‑1 (MQ=255)
                                           agaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCt                           <  1:3026090/71‑1 (MQ=255)
                                             aagTCATTTCCTTTATCATCCACAAGGATAAACGc                                                             >  1:879320/1‑35 (MQ=255)
                                               gTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCt                                                   <  1:2939095/43‑1 (MQ=255)
                                                tCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCa                       <  1:1330090/70‑1 (MQ=255)
                                                 cATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAtt                     >  1:22054/1‑71 (MQ=255)
                                                  aTTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTc                    >  1:3510638/1‑71 (MQ=255)
                                                  aTTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTc                    <  1:1984600/71‑1 (MQ=255)
                                                    ttCCTTTATCATCCACAAGGATAAACGCCGGGAGATCTTCCACTTCaa                                         >  1:2030932/1‑48 (MQ=255)
                                                     accTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATAcc                   >  1:3306792/2‑69 (MQ=255)
                                                        tttGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAgct              >  1:199777/1‑69 (MQ=255)
                                                         ttgtcatcGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTc             >  1:122209/4‑71 (MQ=255)
                                                              atcGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGat        <  1:2037959/71‑1 (MQ=255)
                                                              atcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGa         <  1:3450025/70‑1 (MQ=255)
                                                              atcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGa         >  1:412057/1‑70 (MQ=255)
                                                                  acaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTc    <  1:3181219/71‑1 (MQ=255)
                                                                    aaGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCaa  >  1:2741326/1‑71 (MQ=255)
                                                                    aaGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCaa  <  1:2857610/71‑1 (MQ=255)
                                                                    aaGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCaa  >  1:843968/1‑71 (MQ=255)
                                                                    |                                                                      
TATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAA  >  minE/1014423‑1014561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: