| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,968 | C→T | 14.7% | intergenic (‑60/‑46) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,968 | 0 | C | T | 14.7% | 292.1 / 33.2 | 115 | intergenic (‑60/‑46) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (44/54); new base T (9/8); total (53/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAT > minE/217902‑218036 | aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:2888069/69‑1 (MQ=255)aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:3761061/70‑1 (MQ=255)aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2663366/70‑1 (MQ=255)aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:424125/70‑1 (MQ=255)aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACt > 1:2458313/1‑71 (MQ=255)aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACt > 1:724093/1‑71 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:1390659/71‑1 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:2077113/71‑1 (MQ=255) cATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:245371/66‑1 (MQ=255) taaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg < 1:2942122/71‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:1686334/4‑71 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2987154/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgag < 1:1418205/62‑1 (MQ=255) ggTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa < 1:550551/65‑1 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAtgtg > 1:1451689/2‑60 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAAc > 1:2779023/1‑46 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGc < 1:3013101/71‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:3736631/52‑1 (MQ=255) tCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:2583378/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:1189466/1‑71 (MQ=255) gctgTTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGc < 1:2825032/49‑1 (MQ=255) ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:918029/70‑3 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:2612697/70‑3 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:696136/70‑3 (MQ=255) tgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc > 1:2602757/1‑71 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa > 1:920853/1‑42 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgaga < 1:1378176/55‑1 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:410811/68‑1 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCTGAAACCACTGGCATGTGGAGAATAAGCTg > 1:464516/1‑61 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTc < 1:3655820/71‑1 (MQ=255) ttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:964310/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGga < 1:2104179/49‑1 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3661896/1‑70 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3740812/1‑70 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAc > 1:2507046/1‑42 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa < 1:1274479/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:2699247/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:1126576/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:2536068/1‑71 (MQ=255) ccGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:906744/71‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:3368884/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:2992773/54‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt > 1:2266602/3‑47 (MQ=37) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTgg < 1:1674402/41‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:1954758/38‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:2096358/1‑54 (MQ=255) gagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTCCACTGGCGTCTGGACGTgg > 1:3931825/1‑70 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1641344/68‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:2536499/1‑69 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:2232146/48‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:294466/48‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:1028133/3‑70 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:570190/3‑70 (MQ=255) aattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1675544/69‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:3521627/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1511678/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:2857703/71‑1 (MQ=255) gTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:124471/1‑70 (MQ=39) gTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg > 1:2895760/1‑70 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:2721216/2‑57 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:3447269/69‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:3804863/1‑61 (MQ=38) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:752732/1‑70 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg > 1:1765062/1‑48 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:3808671/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:1816668/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAACCTGCACAGGCGTCTGGACGTGGTaatgaat > 1:3490900/1‑70 (MQ=255) gCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:1425715/1‑70 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga < 1:522549/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2608476/61‑1 (MQ=38) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3806731/69‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat < 1:618958/68‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa < 1:1809217/63‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:3904199/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:1179097/71‑1 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGCAGTGGTAATGAATGAAga > 1:3267468/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2976693/59‑1 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:281643/1‑44 (MQ=38) caGCAATCGGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:392748/1‑71 (MQ=255) caGCAATCCGAAACCCCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1126792/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:252004/53‑1 (MQ=38) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:794802/1‑71 (MQ=39) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:337863/1‑65 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:329316/60‑1 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:341886/70‑1 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:569411/70‑1 (MQ=255) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTa > 1:945283/1‑56 (MQ=255) tCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:522824/63‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1150609/1‑50 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt < 1:194261/43‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGCCGTCTGGACGTGGTaatgaat < 1:183286/59‑1 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTgg > 1:599143/1‑43 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaa < 1:3322932/71‑1 (MQ=39) aaaTCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:173946/1‑71 (MQ=255) aaCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:726669/1‑70 (MQ=39) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAg < 1:877244/58‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAg < 1:388652/58‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2347406/1‑70 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:215781/1‑69 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:3581221/1‑70 (MQ=255) | AACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAT > minE/217902‑218036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |