Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,014,644 T→A 44.9% K475N (AAA→AAT) ‡ fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,014,6440TA44.9% 12.9 / 65.6 49K475N (AAA→AAT) ‡fumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base T (10/17);  new base A (14/8);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 8.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCT  >  minE/1014574‑1014708
                                                                      |                                                                
tctTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGtttt                                                                  >  1:2830426/1‑71 (MQ=255)
   tAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa                                                               >  1:2726405/1‑71 (MQ=255)
    aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa                                                               >  1:522316/1‑70 (MQ=255)
    aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCAAAGGTAGAAGCCGCCGTGtttttt                                                                <  1:1505069/69‑1 (MQ=255)
     aTACTTCCCTACGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTAc                                                              <  1:1032632/70‑1 (MQ=255)
           tgcTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTc                                                       >  1:689321/3‑71 (MQ=255)
           cccTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCa                                                         <  1:1169085/69‑1 (MQ=255)
             cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACacg                                                           >  1:2586187/1‑63 (MQ=255)
             cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGcg                                                         <  1:3625366/67‑2 (MQ=255)
             cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTcgg                                                     >  1:3057193/1‑69 (MQ=255)
                   cagtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACct                                               >  1:1661580/4‑71 (MQ=255)
                    aaTACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa                                                               >  1:1843042/1‑54 (MQ=255)
                     gtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctg                                              >  1:283608/2‑70 (MQ=255)
                      tACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgct                                            >  1:3128585/1‑71 (MQ=255)
                        cAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgctg                                           <  1:3721172/70‑1 (MQ=255)
                           cGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctggctg                                       <  1:2730593/71‑1 (MQ=255)
                           cGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctggct                                        >  1:3006410/1‑70 (MQ=255)
                             gCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCg                                     >  1:1321545/1‑71 (MQ=255)
                              ccGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCgg                                    <  1:421606/71‑1 (MQ=255)
                               cGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGt                                   <  1:1790629/71‑1 (MQ=255)
                                ggACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGtt                                  <  1:367644/71‑1 (MQ=255)
                                ggACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGt                                   >  1:2382111/1‑70 (MQ=255)
                                 gACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTAc                                                              <  1:1575686/42‑1 (MQ=255)
                                  accgccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGttac                                >  1:2005342/1‑69 (MQ=255)
                                  accaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGc                                <  1:2117837/71‑1 (MQ=255)
                                  accaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGc                                >  1:2014914/1‑71 (MQ=255)
                                  accaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGc                                <  1:1255534/71‑1 (MQ=255)
                                   ccaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGt                                                    >  1:3467307/1‑50 (MQ=255)
                                     gccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCtt                             <  1:1882317/70‑1 (MQ=255)
                                      ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCttt                            >  1:3632253/1‑71 (MQ=255)
                                       cGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTc                           <  1:3852099/71‑1 (MQ=255)
                                       cGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTc                           <  1:3375926/71‑1 (MQ=255)
                                          tACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGcc                        <  1:1266158/71‑1 (MQ=255)
                                           aCTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTc                                                   <  1:1152728/43‑1 (MQ=255)
                                             tGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCtt                             <  1:65776/63‑1 (MQ=255)
                                               ccgaGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCAt                   <  1:2384966/68‑1 (MQ=255)
                                               ccgaGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCAt                   <  1:1872678/68‑1 (MQ=255)
                                               ccAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCAt                   >  1:1803096/1‑71 (MQ=255)
                                                cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATg                  <  1:208981/71‑1 (MQ=255)
                                                  aGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGAt                <  1:113469/71‑1 (MQ=255)
                                                   ggTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGAt                >  1:2152047/1‑70 (MQ=255)
                                                      aGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCa              <  1:1381217/69‑1 (MQ=255)
                                                      aGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg            >  1:2552918/1‑70 (MQ=255)
                                                       gAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgc           >  1:582794/1‑69 (MQ=255)
                                                         aGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACtt         <  1:2708418/71‑1 (MQ=255)
                                                          gccgccGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCtgc        >  1:2210878/1‑69 (MQ=255)
                                                            cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg      >  1:3283334/1‑71 (MQ=255)
                                                            cgccgTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCg      >  1:520368/1‑71 (MQ=255)
                                                               cgTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCAt                   <  1:971827/55‑1 (MQ=255)
                                                                gtgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCt  >  1:3815333/1‑71 (MQ=255)
                                                                gtgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCt  <  1:2036719/71‑1 (MQ=255)
                                                                   ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg      >  1:434409/1‑64 (MQ=255)
                                                                     ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCt  >  1:2609980/1‑66 (MQ=255)
                                                                      |                                                                
TCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCT  >  minE/1014574‑1014708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: