| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,996 | A→T | 64.7% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,996 | 0 | A | T | 64.7% | 103.6 / 104.9 | 102 | intergenic (‑88/‑18) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/15); new base T (35/31); total (56/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 | ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:2072619/1‑71 (MQ=255) tgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc > 1:2602757/1‑71 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:410811/68‑1 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTc < 1:3655820/71‑1 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3661896/1‑70 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3740812/1‑70 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa < 1:1274479/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:2699247/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:1126576/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:2536068/1‑71 (MQ=255) ccGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:906744/71‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:3368884/71‑1 (MQ=255) gagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTCCACTGGCGTCTGGACGTgg > 1:3931825/1‑70 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1641344/68‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:2536499/1‑69 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:1028133/3‑70 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt > 1:570190/3‑70 (MQ=255) aattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1675544/69‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:2857703/71‑1 (MQ=255) gTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:124471/1‑70 (MQ=39) gTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg > 1:2895760/1‑70 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:2721216/2‑57 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:3447269/69‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:3804863/1‑61 (MQ=38) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:752732/1‑70 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg > 1:1765062/1‑48 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:3808671/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:1816668/59‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAACCTGCACAGGCGTCTGGACGTGGTaatgaat > 1:3490900/1‑70 (MQ=255) gCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:1425715/1‑70 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga < 1:522549/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:607213/61‑1 (MQ=38) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2608476/61‑1 (MQ=38) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3806731/69‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat < 1:618958/68‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa < 1:1809217/63‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:3257089/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:1179097/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:3904199/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa > 1:876085/1‑54 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGCAGTGGTAATGAATGAAga > 1:3267468/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2976693/59‑1 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:281643/1‑44 (MQ=38) caGCAATCGGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:392748/1‑71 (MQ=255) caGCAATCCGAAACCCCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1126792/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:252004/53‑1 (MQ=38) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:794802/1‑71 (MQ=39) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:337863/1‑65 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:1757602/60‑1 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:329316/60‑1 (MQ=255) aGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1256877/1‑70 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:569411/70‑1 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:341886/70‑1 (MQ=255) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTa > 1:945283/1‑56 (MQ=255) tCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg < 1:522824/63‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1150609/1‑50 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt < 1:194261/43‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGCCGTCTGGACGTGGTaatgaat < 1:183286/59‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa > 1:3422254/1‑61 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacg < 1:3721768/65‑1 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTgg > 1:599143/1‑43 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaa < 1:3322932/71‑1 (MQ=39) aaaTCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:173946/1‑71 (MQ=255) aaCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:726669/1‑70 (MQ=39) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:2709508/1‑39 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAg < 1:388652/58‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAg < 1:877244/58‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2559308/1‑70 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:215781/1‑69 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:3581221/1‑70 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:2103144/53‑1 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaag > 1:1165177/1‑70 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:852031/48‑1 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3582816/48‑1 (MQ=255) tGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa < 1:2315908/43‑1 (MQ=38) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:129343/51‑1 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2237008/1‑64 (MQ=255) ggCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaa > 1:3800580/1‑69 (MQ=255) ggCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaa > 1:3805089/1‑69 (MQ=255) gCACGTGGAGAATAAGCTGCACTGGCGGCTGGACGTGGTAATGAATGAAgac < 1:2671612/52‑1 (MQ=255) atgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAgg > 1:1617337/3‑70 (MQ=255) atgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGAAGACTGCAAAATAAGAAGAGGa < 1:1110959/69‑1 (MQ=255) tgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:2648226/2‑63 (MQ=255) tgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGa > 1:1323050/2‑70 (MQ=255) gTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:253522/1‑71 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTa < 1:3335977/35‑1 (MQ=255) gagaATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa > 1:2505680/1‑42 (MQ=255) agaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacgac > 1:3021510/1‑47 (MQ=255) aaTAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:1004400/1‑46 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1777659/42‑1 (MQ=255) gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:2540746/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat > 1:1681841/1‑70 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:1035807/1‑37 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGCCGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta > 1:2413801/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatta > 1:3952469/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatt > 1:1169153/1‑70 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat < 1:616730/70‑1 (MQ=255) caGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1367906/71‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:3086400/61‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2538903/1‑70 (MQ=255) | CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |