| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,030 | A→G | 59.2% | Y6C (TAC→TGC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,030 | 0 | A | G | 59.2% | 43.2 / 83.6 | 76 | Y6C (TAC→TGC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (19/12); new base G (30/15); total (49/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
GAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTT > minE/217964‑218096 | gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaa < 1:3322932/71‑1 (MQ=39) aaaTCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:173946/1‑71 (MQ=255) aaCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:726669/1‑70 (MQ=39) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2559308/1‑70 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2347406/1‑70 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:215781/1‑69 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:3581221/1‑70 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaag > 1:1165177/1‑70 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:2237008/1‑64 (MQ=255) ggCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaa > 1:3805089/1‑69 (MQ=255) ggCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaa > 1:3800580/1‑69 (MQ=255) atgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAgg > 1:1617337/3‑70 (MQ=255) atgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGAAGACTGCAAAATAAGAAGAGGa < 1:1110959/69‑1 (MQ=255) tgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:2648226/2‑63 (MQ=255) tgTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGa > 1:1323050/2‑70 (MQ=255) gTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:253522/1‑71 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATgcag > 1:2739297/1‑68 (MQ=255) gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:2540746/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat > 1:1681841/1‑70 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGCCGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta > 1:2413801/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatta > 1:3952469/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatt > 1:1169153/1‑70 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat < 1:616730/70‑1 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaag > 1:395554/3‑48 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:299572/3‑71 (MQ=255) caGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1367906/71‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:3086400/61‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2538903/1‑70 (MQ=255) ggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAagaaga > 1:2646695/1‑47 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:3619811/1‑68 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:309878/1‑68 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:3437745/1‑59 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:334325/1‑70 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc > 1:2199733/1‑64 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:3495409/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATgcagc > 1:3611270/1‑50 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACg > 1:535588/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:2514967/55‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa < 1:1624961/69‑1 (MQ=255) aCGTGTTAATTAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg < 1:750625/71‑1 (MQ=255) cGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa > 1:1290340/1‑61 (MQ=255) tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:3189581/1‑70 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3813831/1‑71 (MQ=255) ggTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:907077/1‑71 (MQ=255) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:41141/71‑1 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:2290569/1‑71 (MQ=255) aaatgaatgAAGATGCCAGCCGGATAAGAAGAGGAAACgca < 1:1021342/40‑1 (MQ=255) aatgaatgAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:3231337/1‑71 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa < 1:757557/54‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3676027/1‑55 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc < 1:1563068/71‑1 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCt < 1:1235152/71‑1 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:1358576/1‑52 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGAACATTGCTAt > 1:602004/1‑70 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta < 1:2802961/44‑1 (MQ=38) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg < 1:24334/50‑1 (MQ=39) gaaGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:470995/37‑1 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:133170/1‑70 (MQ=255) cgacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGAt < 1:567610/50‑1 (MQ=38) cgacgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTc < 1:2962830/44‑1 (MQ=38) cgacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:1144971/52‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:814196/48‑1 (MQ=39) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACg < 1:3830853/54‑1 (MQ=255) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattatt < 1:1573422/42‑1 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:121127/1‑71 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:3281020/1‑71 (MQ=255) gacgacTACAAAATAAGAAGAGGGAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:492523/1‑71 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc < 1:2079541/43‑1 (MQ=38) acgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa > 1:204121/1‑48 (MQ=255) acgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:1630863/1‑70 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAttt > 1:125265/1‑71 (MQ=255) cgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa < 1:1548989/43‑1 (MQ=38) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:1274865/1‑71 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:3392271/71‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2097155/70‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:863718/1‑39 (MQ=255) | GAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTT > minE/217964‑218096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |