Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,014,794 G→C 17.3% A425A (GCC→GCG fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,014,7940GC17.3% 116.7 / 17.0 52A425A (GCC→GCGfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (5/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCG  >  minE/1014725‑1014862
                                                                     |                                                                    
gACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTg                                                                      <  1:2334704/70‑1 (MQ=255)
    tAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCgg                                                                 <  1:3038719/71‑1 (MQ=255)
         atccatccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCcg                                                            <  1:2553805/71‑1 (MQ=255)
          tccatccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCgg                                                                 <  1:1531451/65‑1 (MQ=255)
            catacGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCcgcg                                                          <  1:1982954/70‑1 (MQ=255)
             atccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCcgcg                                                          >  1:2802443/1‑69 (MQ=255)
                    cggcggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAAt                                                  <  1:1287834/70‑1 (MQ=255)
                    cggcggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATc                                                 >  1:188493/1‑71 (MQ=255)
                        ggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGgat                                             >  1:3787772/1‑71 (MQ=255)
                         gtggTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtg                                            <  1:2527792/68‑1 (MQ=255)
                         gtggTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtg                                            <  1:2136927/68‑1 (MQ=255)
                              tGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt                                       >  1:2650276/1‑71 (MQ=255)
                               gggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCttt                                      >  1:543991/1‑71 (MQ=255)
                               gggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCTTAGTAAATCGGATGATCtt                                       <  1:2035163/70‑1 (MQ=255)
                               gggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCGGACCCGCGTAGTAAATCGGATGATCttt                                      <  1:2515173/70‑1 (MQ=255)
                                ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtgat                                          >  1:1382077/1‑66 (MQ=255)
                                ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTg                                     <  1:2918340/71‑1 (MQ=255)
                                ggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt                                      <  1:230616/70‑1 (MQ=255)
                                   cAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATg                                  <  1:198368/71‑1 (MQ=255)
                                    aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCgtagtaga                                                   >  1:1545684/1‑51 (MQ=255)
                                    aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGAt                                                  >  1:882680/1‑54 (MQ=255)
                                           ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGAt                                   <  1:1613924/62‑1 (MQ=255)
                                           ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                >  1:3693017/1‑65 (MQ=255)
                                           ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                >  1:1420406/1‑65 (MQ=255)
                                           ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg                          >  1:2969959/1‑71 (MQ=255)
                                           ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg                          >  1:3517909/1‑71 (MQ=255)
                                              gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACt                              >  1:213795/1‑64 (MQ=255)
                                              gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAg                       >  1:2229896/1‑71 (MQ=255)
                                              gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAg                       >  1:3896942/1‑71 (MQ=255)
                                                ggCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGcc                     >  1:2243305/1‑71 (MQ=255)
                                                 gcgtAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGccc                    <  1:2595502/68‑1 (MQ=255)
                                                    tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt                  <  1:2643759/70‑1 (MQ=255)
                                                      aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                >  1:3233718/1‑54 (MQ=255)
                                                       ccTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                <  1:2420601/69‑1 (MQ=255)
                                                       ccTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAcc              >  1:143543/1‑71 (MQ=255)
                                                         ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTc                 <  1:2228676/66‑1 (MQ=255)
                                                          tCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGCCAGCCCTTCACCGt            >  1:488754/1‑70 (MQ=255)
                                                           ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg                          <  1:3254771/55‑1 (MQ=255)
                                                           ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt           <  1:1094924/70‑1 (MQ=255)
                                                           ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt            <  1:108366/69‑1 (MQ=255)
                                                           ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt            <  1:302922/69‑1 (MQ=255)
                                                            cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt           <  1:2704992/69‑1 (MQ=255)
                                                            cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACAGtt           <  1:196842/69‑1 (MQ=255)
                                                              gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcc       <  1:1564775/71‑1 (MQ=255)
                                                              gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatc        >  1:2730299/1‑70 (MQ=255)
                                                               cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcca      >  1:2059890/1‑71 (MQ=255)
                                                                gTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTg                             <  1:3542489/47‑1 (MQ=255)
                                                                gTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTAc                               >  1:713077/1‑45 (MQ=255)
                                                                  tttGGCCGGACCCGCGTAGTAAATCGGATGATCttt                                      <  1:2619144/36‑1 (MQ=255)
                                                                   ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                >  1:1490294/1‑41 (MQ=255)
                                                                   ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg  >  1:1528501/1‑71 (MQ=255)
                                                                   ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg  >  1:3425916/1‑71 (MQ=255)
                                                                   ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg  >  1:1232564/1‑71 (MQ=255)
                                                                     |                                                                    
GACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCG  >  minE/1014725‑1014862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: