| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,794 | G→C | 17.3% | A425A (GCC→GCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,794 | 0 | G | C | 17.3% | 116.7 / 17.0 | 52 | A425A (GCC→GCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (21/22); new base C (5/4); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCG > minE/1014725‑1014862 | gACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTg < 1:2334704/70‑1 (MQ=255) tAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCgg < 1:3038719/71‑1 (MQ=255) atccatccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCcg < 1:2553805/71‑1 (MQ=255) tccatccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCgg < 1:1531451/65‑1 (MQ=255) catacGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCcgcg < 1:1982954/70‑1 (MQ=255) atccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCcgcg > 1:2802443/1‑69 (MQ=255) cggcggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAAt < 1:1287834/70‑1 (MQ=255) cggcggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATc > 1:188493/1‑71 (MQ=255) ggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGgat > 1:3787772/1‑71 (MQ=255) gtggTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtg < 1:2527792/68‑1 (MQ=255) gtggTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtg < 1:2136927/68‑1 (MQ=255) tGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt > 1:2650276/1‑71 (MQ=255) gggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCttt > 1:543991/1‑71 (MQ=255) gggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCTTAGTAAATCGGATGATCtt < 1:2035163/70‑1 (MQ=255) gggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGG‑CGGACCCGCGTAGTAAATCGGATGATCttt < 1:2515173/70‑1 (MQ=255) ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGggtgat > 1:1382077/1‑66 (MQ=255) ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTg < 1:2918340/71‑1 (MQ=255) ggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt < 1:230616/70‑1 (MQ=255) cAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATg < 1:198368/71‑1 (MQ=255) aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCgtagtaga > 1:1545684/1‑51 (MQ=255) aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGAt > 1:882680/1‑54 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGAt < 1:1613924/62‑1 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:3693017/1‑65 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:1420406/1‑65 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg > 1:2969959/1‑71 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg > 1:3517909/1‑71 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACt > 1:213795/1‑64 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAg > 1:2229896/1‑71 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAg > 1:3896942/1‑71 (MQ=255) ggCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGcc > 1:2243305/1‑71 (MQ=255) gcgtAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGccc < 1:2595502/68‑1 (MQ=255) tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt < 1:2643759/70‑1 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:3233718/1‑54 (MQ=255) ccTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa < 1:2420601/69‑1 (MQ=255) ccTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAcc > 1:143543/1‑71 (MQ=255) ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTc < 1:2228676/66‑1 (MQ=255) tCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGCCAGCCCTTCACCGt > 1:488754/1‑70 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg < 1:3254771/55‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:1094924/70‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:108366/69‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:302922/69‑1 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:2704992/69‑1 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACAGtt < 1:196842/69‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcc < 1:1564775/71‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatc > 1:2730299/1‑70 (MQ=255) cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcca > 1:2059890/1‑71 (MQ=255) gTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTg < 1:3542489/47‑1 (MQ=255) gTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTAc > 1:713077/1‑45 (MQ=255) tttGGCCGGACCCGCGTAGTAAATCGGATGATCttt < 1:2619144/36‑1 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:1490294/1‑41 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1528501/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3425916/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1232564/1‑71 (MQ=255) | GACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCG > minE/1014725‑1014862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |