| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,049 | C→T | 48.1% | N12N (AAC→AAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,049 | 0 | C | T | 48.1% | 11.8 / 145.1 | 106 | N12N (AAC→AAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (27/28); new base T (30/21); total (57/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATT > minE/217986‑218114 | gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:2540746/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat > 1:1681841/1‑70 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGCCGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta > 1:2413801/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatta > 1:3952469/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatt > 1:1169153/1‑70 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat < 1:616730/70‑1 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:299572/3‑71 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:3086400/61‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2538903/1‑70 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:309878/1‑68 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:3619811/1‑68 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:3437745/1‑59 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:334325/1‑70 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc > 1:2199733/1‑64 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:3495409/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATgcagc > 1:3611270/1‑50 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACg > 1:535588/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:2514967/55‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa < 1:1624961/69‑1 (MQ=255) aCGTGTTAATTAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg < 1:750625/71‑1 (MQ=255) cGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa > 1:1290340/1‑61 (MQ=255) tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:3189581/1‑70 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3813831/1‑71 (MQ=255) ggTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:907077/1‑71 (MQ=255) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:41141/71‑1 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:2290569/1‑71 (MQ=255) aaatgaatgAAGATGCCAGCCGGATAAGAAGAGGAAACgca < 1:1021342/40‑1 (MQ=255) aatgaatgAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:3231337/1‑71 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa < 1:757557/54‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3676027/1‑55 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc < 1:1563068/71‑1 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCt < 1:1235152/71‑1 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:1358576/1‑52 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGAACATTGCTAt > 1:602004/1‑70 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta < 1:2802961/44‑1 (MQ=38) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg < 1:24334/50‑1 (MQ=39) gaaGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:470995/37‑1 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:133170/1‑70 (MQ=255) cgacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGAt < 1:567610/50‑1 (MQ=38) cgacgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTc < 1:2962830/44‑1 (MQ=38) cgacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:1144971/52‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:814196/48‑1 (MQ=39) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACg < 1:3830853/54‑1 (MQ=255) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattatt < 1:1573422/42‑1 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:3281020/1‑71 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:121127/1‑71 (MQ=255) gacgacTACAAAATAAGAAGAGGGAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:492523/1‑71 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc < 1:2079541/43‑1 (MQ=38) acgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa > 1:204121/1‑48 (MQ=255) acgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat > 1:1630863/1‑70 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAttt > 1:125265/1‑71 (MQ=255) cgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa < 1:1548989/43‑1 (MQ=38) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:1274865/1‑71 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2097155/70‑1 (MQ=255) tgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:1160048/48‑1 (MQ=39) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:863718/1‑39 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGGACATTGCTATTAATATTTTGACg < 1:1249133/70‑1 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:3446679/1‑70 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:2679737/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGAt < 1:1649140/42‑1 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg > 1:1025623/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg < 1:26962/70‑1 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:1587024/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:1962411/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa < 1:3178782/71‑1 (MQ=255) aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa > 1:2943575/1‑71 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCa < 1:2566579/35‑1 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:1162456/1‑71 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:1282403/1‑71 (MQ=255) aaaTAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAAt > 1:2080023/1‑71 (MQ=255) aaaTAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAAt > 1:2635144/1‑71 (MQ=255) aaaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCt > 1:3244053/1‑53 (MQ=255) aaaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCt > 1:3248726/1‑53 (MQ=255) aaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:714974/1‑70 (MQ=255) aTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga > 1:746633/1‑71 (MQ=255) tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa < 1:2759227/38‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag < 1:1561891/71‑1 (MQ=255) aagaagAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag > 1:1398727/1‑70 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:3797051/70‑3 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:629809/70‑3 (MQ=255) agaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga < 1:35170/70‑1 (MQ=255) gaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAat < 1:3589853/53‑1 (MQ=255) gaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAg < 1:3688200/71‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAtt < 1:2948928/43‑1 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTg < 1:3535227/43‑1 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTg < 1:83172/43‑1 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg > 1:3544892/1‑70 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg > 1:1311194/1‑70 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg > 1:3544019/1‑70 (MQ=255) agagGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGt < 1:2237575/71‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTg < 1:2126842/41‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt < 1:148946/71‑1 (MQ=255) agGAAATGCAGCAGAATTATTATCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt > 1:2042261/1‑71 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt > 1:2218775/1‑71 (MQ=255) ggAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTAtt > 1:590098/1‑71 (MQ=255) gAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTa > 1:2488924/1‑68 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:1810703/43‑1 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:1234284/43‑1 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa > 1:3004111/1‑63 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt < 1:3268993/58‑1 (MQ=255) | TAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATT > minE/217986‑218114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |