| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,861 | C→A | 10.7% | R403L (CGG→CTG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,861 | 0 | C | A | 10.7% | 228.5 / 9.0 | 74 | R403L (CGG→CTG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (35/31); new base A (4/4); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01 | |||||||||||
TTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTG > minE/1014792‑1014927 | ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1232564/1‑71 (MQ=255)ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3425916/1‑71 (MQ=255)ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1528501/1‑71 (MQ=255) ccGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCAATCCGctct > 1:2956843/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:1457333/69‑1 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:3216791/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:3115580/1‑70 (MQ=255) aCCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:1319956/1‑69 (MQ=255) cccGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAg > 1:3807735/1‑71 (MQ=255) cgcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGtt < 1:1717469/71‑1 (MQ=255) cgcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3527835/70‑1 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:3558368/71‑1 (MQ=255) cgTAGTAGATCGGGTGATCTTTGATGTACTGCTGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg > 1:1727343/1‑71 (MQ=255) cgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:3640295/1‑65 (MQ=255) tagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGc > 1:3248303/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCTCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2768525/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:1265438/61‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:3070184/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:1093456/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2888457/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2522308/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:3375109/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:2131266/71‑1 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:1791231/58‑1 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:2877466/60‑1 (MQ=255) aaaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGct > 1:2195174/1‑54 (MQ=255) aaaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2008715/1‑69 (MQ=255) taTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:442301/55‑1 (MQ=12) gaTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:1168401/55‑1 (MQ=12) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:744761/54‑1 (MQ=255) tCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGc > 1:101962/1‑70 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCa > 1:3556406/1‑57 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc > 1:1547852/1‑70 (MQ=255) cGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa > 1:3334761/1‑71 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:2937026/1‑71 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:2826406/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:1553129/1‑61 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:1899803/50‑1 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCa > 1:2649052/1‑71 (MQ=255) gatCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc < 1:448763/58‑1 (MQ=12) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATctc > 1:3004617/1‑69 (MQ=255) tttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:2291074/52‑1 (MQ=255) tttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:1098068/52‑1 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:21887/1‑70 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:2362979/1‑69 (MQ=255) tGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg < 1:3493042/67‑1 (MQ=255) tGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccgac < 1:2335525/70‑1 (MQ=255) gTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:3587731/1‑57 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATc < 1:3243381/70‑1 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATc < 1:1534460/70‑1 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACC‑ACGATAATCGt > 1:1410276/1‑71 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt < 1:2026537/71‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc < 1:3929315/71‑1 (MQ=255) aGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc < 1:1662428/71‑1 (MQ=255) aGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc < 1:2207572/71‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:1140457/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:2550910/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:468557/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt < 1:963106/71‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:3145898/45‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:470210/42‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:1592187/1‑67 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:993642/1‑69 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGt > 1:2782717/1‑68 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCa < 1:3006637/39‑1 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3925190/1‑48 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:371294/1‑63 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:1501386/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:606587/1‑71 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCa < 1:3747060/37‑1 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa < 1:2276/71‑1 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3413877/1‑45 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacga > 1:1945741/1‑51 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGAt < 1:1538474/71‑1 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:235065/1‑71 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:527868/1‑71 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:3432997/1‑71 (MQ=255) tccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCg < 1:3866052/71‑1 (MQ=255) ccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt > 1:3147005/1‑52 (MQ=255) catccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCTACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:2392487/70‑1 (MQ=255) catccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:1629771/70‑1 (MQ=255) atccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2052253/1‑70 (MQ=255) atccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:2641121/69‑1 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:595255/1‑69 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2645732/1‑68 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2318366/1‑68 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:525312/1‑68 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:1255571/1‑56 (MQ=255) | TTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTG > minE/1014792‑1014927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |