| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,872 | T→C | 6.3% | K399K (AAA→AAG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,872 | 0 | T | C | 6.3% | 224.6 / 5.0 | 79 | K399K (AAA→AAG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (40/34); new base C (3/2); total (43/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACT > minE/1014803‑1014942 | cgcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGtt < 1:1717469/71‑1 (MQ=255)cgcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3527835/70‑1 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:3558368/71‑1 (MQ=255) cgTAGTAGATCGGGTGATCTTTGATGTACTGCTGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg > 1:1727343/1‑71 (MQ=255) tagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGc > 1:3248303/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCTCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2768525/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:1093456/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:3375109/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:3070184/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2522308/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2888457/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:2131266/71‑1 (MQ=255) aaaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2008715/1‑69 (MQ=255) tCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGc > 1:101962/1‑70 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc > 1:1547852/1‑70 (MQ=255) cGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa > 1:3334761/1‑71 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:2937026/1‑71 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:2826406/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:1553129/1‑61 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCa > 1:2649052/1‑71 (MQ=255) gatCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc < 1:448763/58‑1 (MQ=12) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATctc > 1:3004617/1‑69 (MQ=255) tttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:1098068/52‑1 (MQ=255) tttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:2291074/52‑1 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:21887/1‑70 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:2362979/1‑69 (MQ=255) tGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg < 1:3493042/67‑1 (MQ=255) tGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccgac < 1:2335525/70‑1 (MQ=255) gTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:3587731/1‑57 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATc < 1:1534460/70‑1 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATc < 1:3243381/70‑1 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACC‑ACGATAATCGt > 1:1410276/1‑71 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt < 1:2026537/71‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc < 1:3929315/71‑1 (MQ=255) aGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc < 1:2207572/71‑1 (MQ=255) aGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc < 1:1662428/71‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:1140457/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:468557/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:2550910/45‑1 (MQ=255) ccTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt < 1:963106/71‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:3145898/45‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:470210/42‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:1592187/1‑67 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:993642/1‑69 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGt > 1:2782717/1‑68 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCa < 1:3006637/39‑1 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3925190/1‑48 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:371294/1‑63 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:606587/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:1501386/1‑71 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCa < 1:3747060/37‑1 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa < 1:2276/71‑1 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3413877/1‑45 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacga > 1:1945741/1‑51 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGAt < 1:1538474/71‑1 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:527868/1‑71 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:235065/1‑71 (MQ=255) atccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc > 1:3432997/1‑71 (MQ=255) tccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCg < 1:3866052/71‑1 (MQ=255) ccatccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt > 1:3147005/1‑52 (MQ=255) catccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCTACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:2392487/70‑1 (MQ=255) catccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:1629771/70‑1 (MQ=255) atccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2052253/1‑70 (MQ=255) atccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:2641121/69‑1 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:595255/1‑69 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2645732/1‑68 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:2318366/1‑68 (MQ=255) ccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:525312/1‑68 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:1255571/1‑56 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGc < 1:1072998/62‑1 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg < 1:1867520/65‑1 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa > 1:3617309/1‑71 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa < 1:1391083/71‑1 (MQ=255) tctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:3668642/1‑53 (MQ=255) tctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCAGTTAAGCGATAAGCGTGTAGAAAc > 1:1288027/1‑71 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:1252951/49‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:2670274/69‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:3723039/69‑1 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:1554862/1‑49 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACgg < 1:1525099/69‑1 (MQ=255) aGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATa > 1:1125357/1‑71 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCg < 1:2713305/48‑1 (MQ=255) tttGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACt < 1:1643697/71‑1 (MQ=255) tttGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACt > 1:1771348/1‑71 (MQ=255) tttGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACt < 1:3720232/71‑1 (MQ=255) | CGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACT > minE/1014803‑1014942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |