Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,022 C→T 13.8% E349E (GAG→GAA fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,0220CT13.8% 148.5 / 12.8 58E349E (GAG→GAAfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base T (2/6);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTC  >  minE/1014958‑1015091
                                                                |                                                                     
ggATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTcc                                                                 <  1:3810923/71‑1 (MQ=255)
ggATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCACGCTTTGCGCAGCTCTTcc                                                                 >  1:839054/1‑71 (MQ=255)
  aTCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCtt                                                                   <  1:2651079/67‑1 (MQ=255)
    ctctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGa                                                             >  1:42080/1‑71 (MQ=255)
    ctctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGa                                                             >  1:2393713/1‑71 (MQ=255)
       tttCATCGGACGGTTAAGGTCAACGCCCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGa                                                             <  1:3657820/68‑1 (MQ=255)
          cATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatatat                                                        <  1:2364981/70‑1 (MQ=255)
          cATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGa                                                             <  1:2362131/65‑1 (MQ=255)
             cGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc                                                    >  1:1917220/1‑71 (MQ=255)
              ggACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGcc                                                   >  1:1986686/1‑71 (MQ=255)
              ggACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc                                                    <  1:150915/70‑1 (MQ=255)
                aCGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTg                                                 <  1:1512345/71‑1 (MQ=255)
                   gTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTgggt                                              <  1:1731115/71‑3 (MQ=255)
                     tAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatatat                                                        >  1:3020890/1‑59 (MQ=255)
                     tAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTg                                                 >  1:1941656/1‑66 (MQ=255)
                      aaGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt                                                      <  1:3523031/60‑1 (MQ=255)
                      aaGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTAt                                           >  1:1254363/1‑71 (MQ=255)
                       aGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATg                                          >  1:16646/1‑71 (MQ=255)
                           cAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGtt                                        >  1:2462200/1‑69 (MQ=255)
                            aaCGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGct                                                                      >  1:159842/1‑38 (MQ=255)
                            aaCGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTc                                       >  1:2295325/1‑69 (MQ=255)
                               tttcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGtt                                  >  1:3721710/4‑71 (MQ=255)
                                ttcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt                                 >  1:787777/3‑71 (MQ=255)
                                cgcACCGCTTCGGCTTCTGCCGCTTTTCGCAGCTCTTCCGGGATATATTTGCCTGGATTTTGTTCCAGt                                   <  1:2163730/69‑1 (MQ=255)
                                 tcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt                                 <  1:47357/69‑1 (MQ=255)
                                  cACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGttttt                               >  1:2314172/1‑71 (MQ=255)
                                   aCCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTc                              >  1:3517774/1‑71 (MQ=255)
                                    ccGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCg                             <  1:2290998/71‑1 (MQ=255)
                                     cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa                            <  1:692116/71‑1 (MQ=255)
                                      gCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAtt                                                       <  1:1404141/43‑1 (MQ=255)
                                       cTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATc                          <  1:437175/71‑1 (MQ=255)
                                       cTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt                                 <  1:1406777/64‑1 (MQ=255)
                                          cGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCa                        <  1:1165647/70‑1 (MQ=255)
                                          cGCCTTCTCCCGCTTTGCCCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCa                        <  1:2988440/70‑1 (MQ=255)
                                            ccTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGt                                   <  1:108948/57‑1 (MQ=255)
                                             cTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc                    >  1:1125790/1‑71 (MQ=255)
                                             cTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc                    >  1:1594628/1‑71 (MQ=255)
                                              ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt                                                      <  1:1315451/36‑1 (MQ=255)
                                              ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTTGATTAt                                           <  1:2380/47‑1 (MQ=255)
                                               tctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGt                                         >  1:1497999/1‑48 (MQ=255)
                                                ctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc                    >  1:3728427/1‑68 (MQ=255)
                                                 tcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt                     >  1:1121387/1‑66 (MQ=255)
                                                 tcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt                     >  1:1125932/1‑66 (MQ=255)
                                                   ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGtt                                  <  1:2576907/51‑1 (MQ=255)
                                                   ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTg               <  1:630993/70‑1 (MQ=255)
                                                   ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGa              <  1:1215642/71‑1 (MQ=255)
                                                     gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg            >  1:2299975/1‑71 (MQ=255)
                                                     gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg            >  1:2947824/1‑71 (MQ=255)
                                                     gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg            >  1:1491615/1‑71 (MQ=255)
                                                      cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa                            <  1:3915817/54‑1 (MQ=255)
                                                        ttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt         >  1:3504198/1‑71 (MQ=255)
                                                        ttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt         >  1:1209672/1‑71 (MQ=255)
                                                         tGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGttttt                               <  1:381257/48‑1 (MQ=255)
                                                           cgcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt                <  1:3868742/61‑1 (MQ=255)
                                                            gcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGGTGAtt     <  1:3451835/71‑2 (MQ=255)
                                                            gcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa                            <  1:1638161/48‑1 (MQ=255)
                                                            gcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa       <  1:292661/69‑1 (MQ=255)
                                                              aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt   >  1:1936973/1‑71 (MQ=255)
                                                               gCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTc  >  1:2642122/1‑71 (MQ=255)
                                                                |                                                                     
GGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTC  >  minE/1014958‑1015091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: