| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 13,094 | A→T | 42.1% | D311V (GAT→GTT) | dnaK → | chaperone Hsp70, co‑chaperone with DnaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 13,094 | 0 | A | T | 42.1% | 38.3 / 75.6 | 64 | D311V (GAT→GTT) | dnaK | chaperone Hsp70, co‑chaperone with DnaJ |
| Reads supporting (aligned to +/- strand): ref base A (16/21); new base T (15/12); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATAT > minE/13026‑13163 | cgACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTc < 1:3168501/70‑1 (MQ=255)aGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTc < 1:867047/71‑1 (MQ=255) gACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCt < 1:2969641/71‑1 (MQ=255) gcgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGt < 1:488142/71‑1 (MQ=255) cgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:1962237/71‑1 (MQ=255) ccGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAc > 1:1668595/1‑71 (MQ=255) gTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGt < 1:2857606/71‑1 (MQ=255) gTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGt > 1:102384/1‑71 (MQ=255) gTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGt > 1:3428857/1‑71 (MQ=255) ccGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAAcc < 1:1539028/67‑1 (MQ=255) cGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTg > 1:386296/1‑59 (MQ=255) cGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTg > 1:1377900/1‑59 (MQ=255) aaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAtt > 1:1472583/1‑71 (MQ=255) aaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAtt < 1:2848674/71‑1 (MQ=255) aaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAtt > 1:2634449/1‑71 (MQ=255) aaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt > 1:1551778/1‑70 (MQ=255) acacATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTg < 1:1976498/71‑1 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAg > 1:1318979/1‑69 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGcc > 1:1666927/1‑71 (MQ=255) tGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCg < 1:2955258/71‑1 (MQ=255) tGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCg < 1:2787770/71‑1 (MQ=255) tGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCg < 1:1061704/71‑1 (MQ=255) gAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTg < 1:2158066/65‑1 (MQ=255) aaCATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCt > 1:3711279/1‑71 (MQ=255) aCATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCt > 1:851106/1‑70 (MQ=255) aaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg < 1:2489664/69‑1 (MQ=255) aGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTg > 1:2057838/1‑71 (MQ=255) aCTCGTGCGAAACTGGAAAGCCTGTTTGAAGTTCTGGTAAACCGTTCCAtt < 1:312808/51‑1 (MQ=39) aCTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACt < 1:2809303/71‑1 (MQ=255) cTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTg < 1:2178947/71‑1 (MQ=255) cGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGaaa < 1:1886859/60‑1 (MQ=255) cGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTg < 1:2248659/57‑1 (MQ=255) cGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTg < 1:531207/57‑1 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg < 1:1389751/71‑1 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg < 1:1788155/71‑1 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg < 1:1808803/71‑1 (MQ=255) tGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAc < 1:2409426/37‑1 (MQ=255) tGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAc < 1:2384484/37‑1 (MQ=255) gCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTc > 1:1787033/1‑41 (MQ=255) gCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTc > 1:3487856/1‑41 (MQ=255) cGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTg < 1:1954509/60‑1 (MQ=255) cGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGAc > 1:2969437/1‑71 (MQ=255) gAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:272293/40‑1 (MQ=255) gAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:2380225/40‑1 (MQ=255) gAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACg > 1:545664/1‑71 (MQ=255) aaaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc < 1:2650164/71‑1 (MQ=255) aaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt > 1:3685175/1‑71 (MQ=255) aaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt > 1:63472/1‑71 (MQ=255) aCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt > 1:2292928/1‑39 (MQ=255) aCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt > 1:3167161/1‑39 (MQ=255) aaaGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTg > 1:1666756/1‑71 (MQ=255) ccTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCg > 1:1642706/1‑71 (MQ=255) ggTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTa > 1:2057774/1‑70 (MQ=255) ggTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTAt > 1:3705494/1‑71 (MQ=255) gTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATc > 1:3622389/1‑71 (MQ=255) gtGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTg < 1:3181576/56‑1 (MQ=255) gAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGa > 1:737387/1‑71 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCa > 1:1434344/1‑47 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCa > 1:284808/1‑47 (MQ=255) aGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGata > 1:2007711/1‑71 (MQ=255) aGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGat > 1:1366204/1‑70 (MQ=255) gATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGatat < 1:3320123/71‑1 (MQ=255) gATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGatat < 1:1648480/71‑1 (MQ=255) gATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGatat < 1:2275473/71‑1 (MQ=255) | AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATAT > minE/13026‑13163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |