| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 922,270 | C→T | 7.9% | R114C (CGC→TGC) | ycjZ → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 922,270 | 0 | C | T | 7.9% | 360.7 / 15.0 | 113 | R114C (CGC→TGC) | ycjZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (53/51); new base T (5/4); total (58/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
ATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGT > minE/922206‑922340 | aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt < 1:1452589/71‑1 (MQ=255)aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt < 1:691423/71‑1 (MQ=255)aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt < 1:1758105/71‑1 (MQ=255) cGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTc < 1:447005/70‑1 (MQ=255) aCAATACGTATTACTACTGTAGAACATGCAGCAAAATCGATATTGTTACCAGCAATGCGCACATTCCTGa < 1:3093094/70‑1 (MQ=255) aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa < 1:3106836/71‑1 (MQ=255) aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa < 1:2192937/71‑1 (MQ=255) cAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa < 1:1639679/70‑1 (MQ=255) aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca < 1:551618/59‑1 (MQ=255) aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca < 1:2502288/59‑1 (MQ=255) aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca < 1:743191/59‑1 (MQ=255) aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa < 1:1374955/68‑1 (MQ=255) aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa < 1:3730095/68‑1 (MQ=255) aTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATc < 1:2092342/71‑1 (MQ=255) tACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg > 1:441132/1‑71 (MQ=255) aCGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg > 1:1453972/1‑70 (MQ=255) cGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc < 1:3407616/70‑1 (MQ=255) tactacTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCt > 1:3510836/1‑70 (MQ=255) ctactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcgc > 1:938001/1‑47 (MQ=255) tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa > 1:1841707/1‑71 (MQ=255) tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa > 1:687780/1‑71 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg < 1:1825377/43‑1 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg < 1:2965682/43‑1 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg < 1:1151844/43‑1 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg < 1:2151044/43‑1 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt < 1:1564618/71‑1 (MQ=255) ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt < 1:577180/71‑1 (MQ=255) tGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc > 1:3123399/1‑63 (MQ=255) tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg < 1:503395/69‑1 (MQ=255) gAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGatat < 1:2154915/71‑1 (MQ=255) aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:3275693/1‑70 (MQ=255) aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:2356926/1‑70 (MQ=255) aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTc < 1:107743/71‑1 (MQ=255) cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:3429723/1‑69 (MQ=255) cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:664588/1‑69 (MQ=255) tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa > 1:2233808/1‑57 (MQ=255) tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa > 1:210501/1‑57 (MQ=255) gcagcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGata < 1:2882308/64‑1 (MQ=255) agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:1156599/1‑64 (MQ=255) agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt > 1:1515213/1‑64 (MQ=255) caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc > 1:3011612/1‑70 (MQ=255) caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc > 1:3585991/1‑70 (MQ=255) aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt < 1:2083418/66‑1 (MQ=255) aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa < 1:2131236/71‑1 (MQ=255) aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa > 1:2068546/1‑71 (MQ=255) aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa < 1:430360/71‑1 (MQ=255) aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa > 1:2687009/1‑71 (MQ=255) aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg > 1:3159153/1‑42 (MQ=255) aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc > 1:2009630/1‑43 (MQ=255) aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc > 1:1613448/1‑43 (MQ=255) aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc > 1:2488980/1‑43 (MQ=255) aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa > 1:1921845/1‑52 (MQ=255) aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt > 1:1305410/1‑71 (MQ=255) aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt > 1:751763/1‑71 (MQ=255) aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt > 1:111846/1‑71 (MQ=255) aaaCGATATTGTTACAAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt > 1:3634763/1‑71 (MQ=255) aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAg > 1:1490914/1‑61 (MQ=255) aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt > 1:3555214/1‑70 (MQ=255) aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg < 1:2344720/71‑1 (MQ=255) aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg < 1:1987888/71‑1 (MQ=255) aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg < 1:83746/71‑1 (MQ=255) gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt < 1:2413683/71‑1 (MQ=255) atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa < 1:409118/71‑1 (MQ=255) atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa < 1:3198011/71‑1 (MQ=255) atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa < 1:1301058/71‑1 (MQ=255) atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa < 1:1980024/71‑1 (MQ=255) tatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTAt > 1:1415333/1‑71 (MQ=255) tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt < 1:1766315/62‑1 (MQ=255) tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt < 1:269295/62‑1 (MQ=255) tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg < 1:107499/70‑1 (MQ=255) gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt > 1:3347952/1‑71 (MQ=255) gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt > 1:3402808/1‑71 (MQ=255) ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt < 1:2172813/71‑1 (MQ=255) ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt < 1:1827514/71‑1 (MQ=255) ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt < 1:2433355/71‑1 (MQ=255) ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt < 1:1577755/70‑1 (MQ=255) tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt < 1:863317/38‑1 (MQ=14) tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCAcc > 1:485743/2‑54 (MQ=25) tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc > 1:732965/2‑71 (MQ=38) tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc > 1:1650955/2‑71 (MQ=38) ccAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa < 1:1262701/70‑1 (MQ=255) cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc > 1:616314/1‑71 (MQ=255) cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc > 1:675531/1‑71 (MQ=255) aGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg < 1:663895/71‑1 (MQ=255) gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc > 1:2675162/1‑69 (MQ=255) gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc > 1:2882305/1‑69 (MQ=255) gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc > 1:796053/1‑69 (MQ=255) gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg < 1:2913256/70‑1 (MQ=255) gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGa > 1:780398/1‑71 (MQ=255) gaaTGTGCGCATTGCTGAAATCGCATCCTGAAAtt < 1:2847366/34‑1 (MQ=18) cAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt > 1:189216/1‑71 (MQ=255) aaaTGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt > 1:1458206/2‑71 (MQ=255) aaTGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg < 1:320348/53‑1 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTTATTATGGTTTGACCGATGTc > 1:329376/1‑71 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt > 1:735073/1‑53 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt > 1:1526551/1‑53 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt > 1:2110042/1‑53 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc < 1:1322330/71‑1 (MQ=255) tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc > 1:2091098/1‑71 (MQ=255) cgcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGt > 1:3627474/1‑71 (MQ=255) | ATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGT > minE/922206‑922340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |