Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 922,273 A→G 7.9% T115A (ACA→GCA)  ycjZ → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE922,2730AG 7.9% 324.0 / 14.0 113T115A (ACA→GCA) ycjZpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (54/50);  new base G (5/4);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

ATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTT  >  minE/922206‑922342
                                                                   |                                                                     
aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                                    <  1:1758105/71‑1 (MQ=255)
aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                                    <  1:691423/71‑1 (MQ=255)
aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                                    <  1:1452589/71‑1 (MQ=255)
   cGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTc                                                                  <  1:447005/70‑1 (MQ=255)
      gACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                              <  1:453697/71‑1 (MQ=255)
       aCAATACGTATTACTACTGTAGAACATGCAGCAAAATCGATATTGTTACCAGCAATGCGCACATTCCTGa                                                              <  1:3093094/70‑1 (MQ=255)
       aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                             <  1:2192937/71‑1 (MQ=255)
        cAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                             <  1:1639679/70‑1 (MQ=255)
         aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca                                                                       <  1:743191/59‑1 (MQ=255)
         aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                              <  1:3730095/68‑1 (MQ=255)
         aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                              <  1:1374955/68‑1 (MQ=255)
          aTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATc                                                          <  1:2092342/71‑1 (MQ=255)
           tACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                         >  1:441132/1‑71 (MQ=255)
            aCGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                         >  1:1453972/1‑70 (MQ=255)
             cGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                        <  1:3407616/70‑1 (MQ=255)
                aTTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                                    >  1:2447484/1‑71 (MQ=255)
                  tactacTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCt                                                   >  1:3510836/1‑70 (MQ=255)
                     tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                               >  1:1841707/1‑71 (MQ=255)
                       ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                             <  1:1564618/71‑1 (MQ=255)
                       ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                             <  1:577180/71‑1 (MQ=255)
                        tGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                                    >  1:3123399/1‑63 (MQ=255)
                          tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                            <  1:3134036/69‑1 (MQ=255)
                          tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                            <  1:503395/69‑1 (MQ=255)
                            gAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGatat                                        <  1:2154915/71‑1 (MQ=255)
                              aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                       >  1:2356926/1‑70 (MQ=255)
                              aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTc                                      <  1:107743/71‑1 (MQ=255)
                               cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                       >  1:664588/1‑69 (MQ=255)
                                 tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                                 >  1:2233808/1‑57 (MQ=255)
                                 tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                                 >  1:210501/1‑57 (MQ=255)
                                  gcagcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGata                                         <  1:2882308/64‑1 (MQ=255)
                                    agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                       >  1:1515213/1‑64 (MQ=255)
                                    agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                       >  1:1156599/1‑64 (MQ=255)
                                      caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTccc                               >  1:2982144/1‑68 (MQ=255)
                                      caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                               >  1:1810130/1‑70 (MQ=255)
                                      caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                               >  1:3585991/1‑70 (MQ=255)
                                      caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                               >  1:3011612/1‑70 (MQ=255)
                                       aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt                                  <  1:2083418/66‑1 (MQ=255)
                                       aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                             >  1:2068546/1‑71 (MQ=255)
                                       aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                             >  1:2687009/1‑71 (MQ=255)
                                        aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                         >  1:3159153/1‑42 (MQ=255)
                                        aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                        >  1:2009630/1‑43 (MQ=255)
                                        aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                        >  1:1613448/1‑43 (MQ=255)
                                        aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                        >  1:2488980/1‑43 (MQ=255)
                                        aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                               >  1:1921845/1‑52 (MQ=255)
                                         aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                           >  1:1305410/1‑71 (MQ=255)
                                         aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                           >  1:111846/1‑71 (MQ=255)
                                         aaaCGATATTGTTACAAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                           >  1:3634763/1‑71 (MQ=255)
                                          aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAg                                    >  1:1490914/1‑61 (MQ=255)
                                          aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                           >  1:3555214/1‑70 (MQ=255)
                                          aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                          <  1:83746/71‑1 (MQ=255)
                                          aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                          <  1:1987888/71‑1 (MQ=255)
                                             gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                       <  1:2413683/71‑1 (MQ=255)
                                             gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                       <  1:2140374/71‑1 (MQ=255)
                                              atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                      <  1:3198011/71‑1 (MQ=255)
                                              atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                      <  1:1980024/71‑1 (MQ=255)
                                              atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                      <  1:409118/71‑1 (MQ=255)
                                              atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                      <  1:1301058/71‑1 (MQ=255)
                                               tatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTAt                     >  1:1415333/1‑71 (MQ=255)
                                                  tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                           <  1:269295/62‑1 (MQ=255)
                                                  tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg                   <  1:107499/70‑1 (MQ=255)
                                                   gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                 >  1:3347952/1‑71 (MQ=255)
                                                   gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                 >  1:3402808/1‑71 (MQ=255)
                                                    ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                <  1:1827514/71‑1 (MQ=255)
                                                    ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                <  1:2433355/71‑1 (MQ=255)
                                                    ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                <  1:2172813/71‑1 (MQ=255)
                                                    ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                 <  1:1577755/70‑1 (MQ=255)
                                                      ttcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa              >  1:3327062/3‑71 (MQ=37)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                             <  1:3731231/38‑1 (MQ=14)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                             <  1:863317/38‑1 (MQ=14)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCAcc                              >  1:485743/2‑54 (MQ=25)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc             >  1:732965/2‑71 (MQ=38)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc             >  1:698845/2‑71 (MQ=38)
                                                       tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc             >  1:1650955/2‑71 (MQ=38)
                                                       ccAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa              <  1:1262701/70‑1 (MQ=255)
                                                        cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            >  1:675531/1‑71 (MQ=255)
                                                        cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            >  1:2710616/1‑71 (MQ=255)
                                                        cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            >  1:616314/1‑71 (MQ=255)
                                                         aggaaTGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            <  1:3541301/67‑1 (MQ=255)
                                                         aGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg           <  1:663895/71‑1 (MQ=255)
                                                          gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            >  1:2675162/1‑69 (MQ=255)
                                                          gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc            >  1:796053/1‑69 (MQ=255)
                                                          gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg           <  1:2913256/70‑1 (MQ=255)
                                                          gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGa          >  1:780398/1‑71 (MQ=255)
                                                           gaaTGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                             <  1:2847366/34‑1 (MQ=18)
                                                           cAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt         >  1:189216/1‑71 (MQ=255)
                                                           aaaTGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt         >  1:1458206/2‑71 (MQ=255)
                                                            aaTGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                          <  1:320348/53‑1 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTTATTATGGTTTGACCGATGTc      >  1:329376/1‑71 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                        >  1:735073/1‑53 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                        >  1:2110042/1‑53 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                        >  1:1526551/1‑53 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc      <  1:1322330/71‑1 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc      <  1:785327/71‑1 (MQ=255)
                                                              tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc      >  1:2091098/1‑71 (MQ=255)
                                                                 gcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGtt   <  1:3704649/71‑1 (MQ=255)
                                                                 gcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGtt   <  1:1217657/71‑1 (MQ=255)
                                                                  cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt  <  1:1748193/71‑1 (MQ=255)
                                                                  cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt  <  1:176142/71‑1 (MQ=255)
                                                                  cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt  >  1:2091369/1‑71 (MQ=255)
                                                                  cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt  <  1:345773/71‑1 (MQ=255)
                                                                   |                                                                     
ATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTT  >  minE/922206‑922342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: