| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 963,892 | C→T | 13.5% | G185E (GGA→GAA) | yddA ← | fused predicted multidrug transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 963,892 | 0 | C | T | 13.5% | 199.4 / 19.8 | 74 | G185E (GGA→GAA) | yddA | fused predicted multidrug transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (40/24); new base T (4/6); total (44/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
AATAAAGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCAT > minE/963823‑963956 | aataaaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTc < 1:353859/70‑1 (MQ=255) taaaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccac > 1:1918882/1‑71 (MQ=255) taaaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccac > 1:297340/1‑71 (MQ=255) taaaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccac > 1:3022728/1‑71 (MQ=255) taaaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccac > 1:3008636/1‑71 (MQ=255) aaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccacc > 1:3082025/1‑70 (MQ=255) aaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTccacc < 1:1193123/70‑1 (MQ=255) aaGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTcc > 1:3077975/1‑67 (MQ=255) aCCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGa < 1:3145822/71‑1 (MQ=255) ccGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGaa > 1:3281418/1‑71 (MQ=255) cGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAAt > 1:114537/1‑71 (MQ=255) cGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAAt > 1:2439700/1‑71 (MQ=255) gATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATg < 1:2262445/71‑1 (MQ=255) acaATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGagag > 1:331767/1‑71 (MQ=255) aTGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCg > 1:3101595/1‑70 (MQ=255) aTGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCg > 1:251432/1‑70 (MQ=255) tGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATgaga > 1:3605538/1‑66 (MQ=255) gtgCACGACTGTATAGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATg < 1:2497753/60‑1 (MQ=255) aGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTTAATGAGAGCGTAc < 1:1042965/70‑1 (MQ=255) gCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAccc > 1:482878/1‑71 (MQ=255) gCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAccc > 1:606378/1‑71 (MQ=255) gCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAccc > 1:1114057/1‑71 (MQ=255) gCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAc < 1:232863/69‑1 (MQ=255) cgacgGTATAGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCcgcg < 1:3710347/71‑1 (MQ=255) cgacgGTATAGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCcgcg > 1:3514133/1‑71 (MQ=255) cgacgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCcgcg > 1:2841334/1‑71 (MQ=255) gacgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCgcgc > 1:2804094/1‑71 (MQ=255) cgGTATAGACCATATACCCCTGGATATTCCATTCTGTTccac < 1:1276002/42‑1 (MQ=255) cgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGt > 1:2252955/1‑49 (MQ=255) cgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGt > 1:3690691/1‑49 (MQ=255) cgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGt > 1:567657/1‑49 (MQ=255) cgGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGt > 1:696609/1‑49 (MQ=255) gTATAGACCATATACCCCTGGATATTCCATTCTGTTccacca > 1:3401334/1‑42 (MQ=255) gTATAGACCATATACCCCTGGATATTCCATTCTGTTccacca > 1:1195635/1‑42 (MQ=255) tataGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCCGCGCTTTcc > 1:1358984/1‑71 (MQ=255) tataGACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCCGCGCTTTcc > 1:1183702/1‑71 (MQ=255) tataGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGa < 1:933529/48‑1 (MQ=255) tataGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGa < 1:2847069/48‑1 (MQ=255) tataGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGa < 1:1127475/48‑1 (MQ=255) ataGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAcc > 1:2348147/1‑60 (MQ=255) aGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCa > 1:42986/1‑70 (MQ=255) gACCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCaa < 1:2587293/70‑1 (MQ=255) gACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCaaa < 1:3457881/71‑1 (MQ=255) gACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCaaa < 1:1652087/71‑1 (MQ=255) aCCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGt < 1:2204546/53‑1 (MQ=255) aCCATATACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGt < 1:2381144/53‑1 (MQ=255) aCCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCaaa < 1:1627477/70‑1 (MQ=255) ccATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCTCGCTTTCCCAAAg > 1:3136698/1‑70 (MQ=255) ccATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAg > 1:1457225/1‑70 (MQ=255) ccATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAg > 1:3209574/1‑70 (MQ=255) atatACCCCTGGATATTCCATTCTGTTTCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGaa > 1:3395169/1‑70 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:2821814/39‑1 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:3030261/39‑1 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:453833/39‑1 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:1895641/39‑1 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:1652406/39‑1 (MQ=255) atatACCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:471169/39‑1 (MQ=255) ataCCCCTGGATATTCCATTCTGTTCCACCAACAGTg < 1:862637/37‑1 (MQ=255) ataCCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATaa > 1:2231992/1‑71 (MQ=255) taCCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTAc > 1:3453023/1‑49 (MQ=255) aCCCCTGGATATTCCATTCTGTTCCACCAACAGTGaa > 1:742608/1‑37 (MQ=255) aCCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACg > 1:910631/1‑71 (MQ=255) ccccTGGATATTCCATTCTGTTCCACCAACAGTGAAt > 1:1102282/1‑37 (MQ=255) ccccTGGATATTCCATTCTGTTCCACCAACAGTGAAt > 1:1749476/1‑37 (MQ=255) ccccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACgg > 1:2693313/1‑71 (MQ=255) cccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCtt < 1:2182289/54‑1 (MQ=255) cccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATa > 1:877313/1‑66 (MQ=255) cccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACgg > 1:239867/1‑70 (MQ=255) cccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACgg > 1:1147880/1‑70 (MQ=255) ccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTa < 1:409311/71‑1 (MQ=255) ccTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTa < 1:499065/71‑1 (MQ=255) cTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTc > 1:2482576/1‑54 (MQ=255) ggATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCg > 1:625578/1‑39 (MQ=255) gATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAgg > 1:874836/1‑71 (MQ=255) ttCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGAt > 1:1831974/1‑71 (MQ=255) ttCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGAt > 1:715159/1‑71 (MQ=255) ccATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGAt > 1:1301256/1‑69 (MQ=255) ccATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGAt > 1:2401252/1‑69 (MQ=255) cATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCa < 1:3437590/70‑1 (MQ=255) cATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCa > 1:1292525/1‑70 (MQ=255) cATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCa < 1:235249/70‑1 (MQ=255) aTTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGc < 1:1121710/71‑1 (MQ=255) ttCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCa < 1:3056206/71‑1 (MQ=255) tCTGTTCCACCAACAGTGAATGAGAGCGTACCCGAGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCAt > 1:3140799/1‑71 (MQ=255) gTTCCAGCAACAGTGAATGAGAGCGTACCCGCGCt < 1:2927869/35‑1 (MQ=255) | AATAAAGTTCCACCGATCACAATGAGCACGACGGTATAGACCATATACCCCTGGATATTCCATTCTGTTCCACCAACAGTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCAT > minE/963823‑963956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |