| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,019,797 | T→C | 57.1% | Y249C (TAT→TGT) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,019,797 | 0 | T | C | 57.1% | 27.7 / 77.0 | 63 | Y249C (TAT→TGT) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base T (17/10); new base C (15/21); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
GCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACCG > minE/1019729‑1019866 | gctggctgGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATaa > 1:3649677/1‑71 (MQ=255) gctggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAAtt < 1:2220009/69‑1 (MQ=255) ctggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCgg < 1:2286086/71‑1 (MQ=255) tggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCgg < 1:2595237/70‑1 (MQ=255) ggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAG‑AACTCCCCTTTCAACGTACAATTCGGGTc > 1:2092223/1‑71 (MQ=255) ttACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAg < 1:2831286/71‑1 (MQ=255) ttACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAg < 1:809187/71‑1 (MQ=255) tACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAg < 1:2534458/70‑1 (MQ=255) tACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGc > 1:1628421/1‑71 (MQ=255) aCGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCg > 1:1854106/1‑71 (MQ=255) cGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCg < 1:1933475/70‑1 (MQ=255) cGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCg < 1:30786/70‑1 (MQ=255) cGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg < 1:2210368/71‑1 (MQ=255) cGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg < 1:1060070/71‑1 (MQ=255) gACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg < 1:649895/70‑1 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTa > 1:3600770/1‑71 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg > 1:2324727/1‑69 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg > 1:3453046/1‑69 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTa < 1:2409716/71‑1 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTa < 1:1982891/71‑1 (MQ=255) cTTAAACCTTCGAGCTGCGAGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTAt > 1:2626501/1‑71 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATg < 1:2099307/69‑1 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGc < 1:1546340/70‑1 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCt > 1:3181398/1‑71 (MQ=255) aaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCtt < 1:1354135/71‑1 (MQ=255) aaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCtt < 1:1384971/71‑1 (MQ=255) ccTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCtttt > 1:3332856/1‑71 (MQ=255) ccTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCtttt < 1:2836429/71‑1 (MQ=255) tCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtctc > 1:2022815/1‑71 (MQ=255) cGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtct < 1:286971/69‑1 (MQ=255) gAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg > 1:49127/1‑57 (MQ=255) gAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg > 1:451588/1‑57 (MQ=255) cTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGt < 1:1998680/55‑1 (MQ=255) cTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGt < 1:871911/55‑1 (MQ=255) cTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGaa > 1:2849894/1‑71 (MQ=255) tAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTg > 1:2340466/1‑71 (MQ=255) aaaGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTa > 1:470783/1‑71 (MQ=255) aaaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCg < 1:3034856/40‑1 (MQ=255) aaaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATAc < 1:3285622/65‑1 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGt < 1:3406436/45‑1 (MQ=255) aGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATc > 1:729289/1‑71 (MQ=255) aGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATAc < 1:2417110/63‑1 (MQ=255) aGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt < 1:1896183/66‑1 (MQ=255) aGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt < 1:1914659/66‑1 (MQ=255) aaCTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTgg > 1:1205350/1‑70 (MQ=255) aaCTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTgg > 1:2780985/1‑70 (MQ=255) cTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCga < 1:1430437/47‑1 (MQ=255) ccccTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCgaga > 1:3124333/1‑47 (MQ=255) ccccTTTCAACGTATAATTCGGCTCGAGCGGTATGCTTTTCTCgaga > 1:2798339/1‑47 (MQ=255) ccccTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACa > 1:2084128/1‑71 (MQ=255) cccTTTCAACGTATAATTCGGGTCGAGCGGTATGCt < 1:1905974/36‑1 (MQ=255) cTTTCAACGTATAATTCGGGTCGAGCGGTATGCttt > 1:3165543/1‑36 (MQ=255) cTTTCAACGTATAATTCGGGTCGAGCGGTATGCttt > 1:2004371/1‑36 (MQ=255) cTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCt > 1:957375/1‑71 (MQ=255) tttCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACtt > 1:924889/1‑49 (MQ=255) tttCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCtt > 1:503833/1‑71 (MQ=255) tCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCga > 1:1575813/1‑39 (MQ=255) tCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCga > 1:1244738/1‑39 (MQ=255) cAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCt > 1:2462248/1‑71 (MQ=255) cAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCt > 1:1954878/1‑71 (MQ=255) aCGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTg < 1:3154245/70‑1 (MQ=255) cGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACt < 1:3486623/42‑1 (MQ=255) gTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATATTTCTGTa > 1:3099202/1‑70 (MQ=255) taATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACCg < 1:338987/70‑1 (MQ=255) taATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACCg < 1:1383371/70‑1 (MQ=255) | GCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACCG > minE/1019729‑1019866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |