Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,038,735 | G→C | 59.8% | V73L (GTG→CTG) | rsxE → | predicted inner membrane NADH‑quinone reductase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,038,735 | 0 | G | C | 59.8% | 69.0 / 121.9 | 107 | V73L (GTG→CTG) | rsxE | predicted inner membrane NADH‑quinone reductase |
Reads supporting (aligned to +/- strand): ref base G (24/19); new base C (20/44); total (44/63) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.60e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 107 total aligned reads displayed.
ACGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG > minE/1038666‑1038800 | aCGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGt < 1:2871308/71‑1 (MQ=255) cGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGt > 1:1060345/1‑70 (MQ=255) cTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat < 1:1763939/71‑1 (MQ=255) tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGtgat > 1:2699325/1‑70 (MQ=255) tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat > 1:2114961/1‑70 (MQ=255) tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat > 1:1812740/1‑70 (MQ=255) tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat > 1:3315206/1‑70 (MQ=255) gACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgatga > 1:3384124/1‑71 (MQ=255) aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat > 1:1819989/1‑71 (MQ=255) aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat > 1:582678/1‑71 (MQ=255) aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat > 1:493822/1‑71 (MQ=255) aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat > 1:2442905/1‑71 (MQ=255) ccTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCg > 1:433561/1‑71 (MQ=255) ccTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCg > 1:1744135/1‑71 (MQ=255) cTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGc > 1:3614882/1‑71 (MQ=255) cTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGc > 1:1249188/1‑71 (MQ=255) gACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCt > 1:3634522/1‑71 (MQ=255) aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTc > 1:3158658/1‑71 (MQ=255) aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCt > 1:945309/1‑70 (MQ=255) cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCgg < 1:1170167/71‑1 (MQ=255) cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCgg < 1:2877260/71‑1 (MQ=255) cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg < 1:2627061/71‑1 (MQ=255) cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg < 1:2030960/71‑1 (MQ=255) cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg < 1:2018862/71‑1 (MQ=255) aTTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggt < 1:3456884/71‑1 (MQ=255) tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg > 1:2340059/1‑71 (MQ=255) tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg > 1:2234767/1‑71 (MQ=255) tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg > 1:2709524/1‑71 (MQ=255) tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg > 1:2136135/1‑71 (MQ=255) tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCg < 1:1771481/68‑1 (MQ=255) cGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtggt < 1:2332988/70‑1 (MQ=255) cGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt < 1:3044074/70‑1 (MQ=255) gACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTc < 1:3177337/70‑1 (MQ=255) gACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTc < 1:598320/70‑1 (MQ=255) aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga < 1:1478488/49‑1 (MQ=255) aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga < 1:866229/49‑1 (MQ=255) aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga < 1:1192201/49‑1 (MQ=255) gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt < 1:114355/66‑1 (MQ=255) gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg < 1:1991488/71‑1 (MQ=255) gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg < 1:615538/71‑1 (MQ=255) gCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTg > 1:3222801/1‑71 (MQ=255) tCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTAc < 1:54403/71‑1 (MQ=255) cACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTa > 1:2113378/1‑69 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa < 1:484980/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa < 1:1684116/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa < 1:40407/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa < 1:2837403/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg < 1:2705240/71‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGGTGTACa < 1:5394/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa < 1:82378/70‑1 (MQ=255) aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg < 1:3595778/71‑1 (MQ=255) cTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg < 1:2279754/70‑1 (MQ=255) gACGCCAGCGGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt < 1:189393/69‑1 (MQ=255) gCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGa < 1:2086589/71‑1 (MQ=255) ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt > 1:1940629/1‑71 (MQ=255) ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt > 1:1549743/1‑71 (MQ=255) ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt > 1:3164054/1‑71 (MQ=255) cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc < 1:3348732/71‑1 (MQ=255) cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc < 1:2415364/71‑1 (MQ=255) cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc < 1:1780661/71‑1 (MQ=255) aGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCa < 1:609814/71‑1 (MQ=255) aGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCa < 1:2255450/71‑1 (MQ=255) ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa > 1:879754/1‑70 (MQ=255) ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac < 1:3154803/71‑1 (MQ=255) ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac > 1:1099785/1‑71 (MQ=255) ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac > 1:1669324/1‑71 (MQ=255) ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac < 1:2861825/71‑1 (MQ=255) ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac < 1:1384132/71‑1 (MQ=255) ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac < 1:2757765/71‑1 (MQ=255) gaTCCGCATTCCCATTTACGTGATGATCATCGCCTCggtggt < 1:2781840/42‑1 (MQ=255) gaTCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc > 1:1733212/1‑70 (MQ=255) tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc > 1:3668410/1‑62 (MQ=255) tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta > 1:505073/1‑70 (MQ=255) tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta > 1:1568368/1‑70 (MQ=255) ccGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctacg > 1:1475089/1‑71 (MQ=255) cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc < 1:3254201/66‑1 (MQ=255) cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc < 1:3519515/66‑1 (MQ=255) cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc < 1:3070681/66‑1 (MQ=255) cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc < 1:556566/66‑1 (MQ=255) cATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctacgcct < 1:868664/71‑1 (MQ=255) aTTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt > 1:2817901/1‑71 (MQ=255) aTTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt > 1:1032804/1‑71 (MQ=255) aTTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt < 1:535884/71‑1 (MQ=255) ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa > 1:3524860/1‑58 (MQ=255) ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa > 1:1159615/1‑58 (MQ=255) ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt < 1:3305161/70‑1 (MQ=255) ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt < 1:1571124/70‑1 (MQ=255) ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt < 1:381315/52‑1 (MQ=255) ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt < 1:2599601/67‑1 (MQ=255) ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc < 1:1039573/71‑1 (MQ=255) ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc < 1:3505055/71‑1 (MQ=255) cATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc < 1:1312694/70‑1 (MQ=255) aTTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc < 1:2023610/51‑1 (MQ=255) aTTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc < 1:1339554/51‑1 (MQ=255) tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta > 1:2949435/1‑58 (MQ=255) tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta > 1:1888100/1‑58 (MQ=255) tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta > 1:1179997/1‑58 (MQ=255) tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg > 1:3255312/1‑71 (MQ=255) tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGa < 1:947949/48‑1 (MQ=255) tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg < 1:3601808/71‑1 (MQ=255) | ACGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG > minE/1038666‑1038800 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |