| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,044,955 | A→G | 13.4% | intergenic (‑42/+87) | tyrS ← / ← pdxH | tyrosyl‑tRNA synthetase/pyridoxine 5'‑phosphate oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,044,955 | 0 | A | G | 13.4% | 175.7 / 17.8 | 67 | intergenic (‑42/+87) | tyrS/pdxH | tyrosyl‑tRNA synthetase/pyridoxine 5'‑phosphate oxidase |
| Reads supporting (aligned to +/- strand): ref base A (29/29); new base G (4/5); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
TTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGC > minE/1044887‑1045024 | ttGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac > 1:2146391/1‑70 (MQ=255)ttGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac > 1:109237/1‑70 (MQ=255)ttGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac > 1:2248546/1‑70 (MQ=255)ttGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac > 1:1576237/1‑70 (MQ=255)ttGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac > 1:3564189/1‑70 (MQ=255) gTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGAc > 1:3159018/1‑71 (MQ=255) gTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGAc > 1:636725/1‑71 (MQ=255) aaTCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt < 1:2383362/70‑1 (MQ=255) aaTCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt < 1:2205716/70‑1 (MQ=255) aaTCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt < 1:461035/70‑1 (MQ=255) tCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg < 1:3543091/71‑1 (MQ=255) tCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg > 1:927420/1‑71 (MQ=255) tCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg < 1:1983021/71‑1 (MQ=255) cAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc > 1:3123536/1‑71 (MQ=255) aaGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc > 1:887728/1‑70 (MQ=255) gTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg < 1:195349/71‑1 (MQ=255) gTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCCTTGCCGGTacac < 1:2643041/58‑1 (MQ=255) tGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACGCGACTTTTCGCCAGATGc > 1:542440/1‑70 (MQ=255) gCTTGCCATCAAAATCTCCTTGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATg < 1:1134741/68‑1 (MQ=255) gCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGa > 1:1858713/1‑54 (MQ=255) gCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGa > 1:1854171/1‑54 (MQ=255) gCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc > 1:3217698/1‑58 (MQ=255) cATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACGCGACTTTTCGCCAGATGCGAAAgaga > 1:1135932/1‑71 (MQ=255) cATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa < 1:3509878/67‑1 (MQ=255) aTCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc < 1:1013372/53‑1 (MQ=255) tCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACa < 1:1030295/71‑1 (MQ=255) cAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc > 1:1627586/1‑53 (MQ=255) aaaTCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg > 1:714954/1‑50 (MQ=255) aaaTCTCCATGTATATAAAGACTGCACCTTTGGCGGTACACGACTTTTCGCCAGATGCGAAAGAGAc > 1:488988/1‑67 (MQ=255) aaTCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAg > 1:425697/1‑70 (MQ=255) aaTCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAg > 1:395220/1‑70 (MQ=255) ccATGTATATAACGACTGCACCTTTGCCGGTACACGACttt < 1:899482/41‑1 (MQ=255) cATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaaa < 1:3263740/70‑1 (MQ=255) gTATATAACGACTGCACCTTTGCCGGTACGCGACTTTTCGCCAGATg < 1:778165/47‑1 (MQ=255) tatataACGACTGCACCTTTGCCGGTACGCGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGc < 1:2463067/70‑1 (MQ=255) tatataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTg > 1:333542/1‑69 (MQ=255) tataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGc > 1:2990815/1‑68 (MQ=255) tataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGc > 1:3004517/1‑68 (MQ=255) tataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGc > 1:781958/1‑68 (MQ=255) tataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCa < 1:578645/70‑1 (MQ=255) ataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa < 1:1984664/46‑1 (MQ=255) ataACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATa > 1:777057/1‑56 (MQ=255) aaCGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGt < 1:3044900/63‑1 (MQ=255) cGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGAc < 1:30832/49‑1 (MQ=255) cGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGAc < 1:2321680/49‑1 (MQ=255) tGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa > 1:1551072/1‑40 (MQ=255) ccTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaaa < 1:466845/51‑1 (MQ=255) ccTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaaa < 1:401103/51‑1 (MQ=255) cTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaaa < 1:1558348/50‑1 (MQ=255) cTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAg < 1:2242428/63‑1 (MQ=255) cTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTAc > 1:2154898/1‑69 (MQ=255) tttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGa < 1:904143/57‑1 (MQ=255) tttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGa < 1:2269614/57‑1 (MQ=255) ttGCCGGTACGCGACTTTTCGCCAGATGCGAAAGAGAc > 1:2809931/1‑38 (MQ=255) ttGCCGGTACGCGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAg > 1:858025/1‑64 (MQ=255) ttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATa > 1:629416/1‑46 (MQ=255) ttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTAcc > 1:2904423/1‑68 (MQ=255) ttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGc > 1:2574006/1‑71 (MQ=255) ttGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGc > 1:2569628/1‑71 (MQ=255) tGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGa > 1:8356/1‑42 (MQ=255) tGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGa > 1:3029059/1‑42 (MQ=255) gCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGt > 1:1392438/1‑48 (MQ=255) ccGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAt < 1:3045747/71‑1 (MQ=255) cGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAtt < 1:482717/71‑1 (MQ=255) cGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAtt < 1:998587/71‑1 (MQ=255) cGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAt > 1:3195147/1‑70 (MQ=255) ggTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAtt > 1:25456/1‑70 (MQ=255) ggTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAtt < 1:1297389/70‑1 (MQ=255) ggTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTa < 1:3632005/71‑1 (MQ=255) ggTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTa < 1:289119/71‑1 (MQ=255) gTACGCGACTTTTCGCCAGATGCGAAAGAGACATAGAATa < 1:2635200/40‑1 (MQ=255) gTACGCGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCa < 1:735818/63‑1 (MQ=255) gTACGCGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCa < 1:1539058/63‑1 (MQ=255) acacGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGa > 1:473501/1‑65 (MQ=255) cacGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAg < 1:2662712/70‑1 (MQ=255) cacGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGc < 1:2314705/71‑1 (MQ=255) | TTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGC > minE/1044887‑1045024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |