| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,091,167 | T→C | 52.1% | E178E (GAA→GAG) | sufB ← | component of SufBCD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,091,167 | 0 | T | C | 52.1% | 1.5 / 67.1 | 48 | E178E (GAA→GAG) | sufB | component of SufBCD complex |
| Reads supporting (aligned to +/- strand): ref base T (12/11); new base C (15/10); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAT > minE/1091100‑1091237 | ccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTcc < 1:2071346/71‑1 (MQ=255) gcATTAAGCGCGTCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg < 1:2060041/71‑1 (MQ=255) gcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCgg < 1:2625242/71‑1 (MQ=255) gcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg < 1:1359093/71‑1 (MQ=255) aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGAt > 1:676362/1‑71 (MQ=255) aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGAt > 1:225160/1‑71 (MQ=255) cgcgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGt < 1:2702694/71‑1 (MQ=255) cgcgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGt < 1:1617315/71‑1 (MQ=255) cAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATc < 1:2078114/71‑1 (MQ=255) cAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATc < 1:1978844/71‑1 (MQ=255) agaagTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGcc > 1:3530986/1‑71 (MQ=255) tGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCAcc > 1:2514481/1‑70 (MQ=255) gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCg > 1:2676701/1‑55 (MQ=255) gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg > 1:1744903/1‑48 (MQ=255) tCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGAt < 1:1047754/52‑1 (MQ=255) aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAgg < 1:1419729/71‑1 (MQ=255) aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGAt < 1:3503460/57‑1 (MQ=255) aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAg < 1:2451826/70‑1 (MQ=255) ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCa > 1:1770516/1‑63 (MQ=255) ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCa > 1:1812430/1‑63 (MQ=255) ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGa < 1:1128103/71‑1 (MQ=255) cGGCACCGTGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAGCAgg > 1:2015725/1‑70 (MQ=255) cGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGa < 1:1068632/71‑1 (MQ=255) caccacGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaaaa < 1:2514225/71‑1 (MQ=255) gtggTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCaa < 1:3203599/53‑1 (MQ=255) acGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataat > 1:494505/1‑71 (MQ=255) cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt > 1:1030784/1‑71 (MQ=255) ggTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCAcc > 1:1490792/1‑51 (MQ=255) ggTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTc > 1:1011953/1‑71 (MQ=255) tGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc < 1:1328921/57‑1 (MQ=255) tGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTccc < 1:3258934/71‑1 (MQ=255) cGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc < 1:2494855/54‑1 (MQ=255) gagaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCt > 1:3232024/1‑71 (MQ=255) gagaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCt > 1:535695/1‑71 (MQ=255) gaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTcc > 1:930958/1‑71 (MQ=255) gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:2012162/1‑51 (MQ=255) gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:366854/1‑51 (MQ=255) gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:1761208/1‑51 (MQ=255) gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTcc > 1:974280/1‑71 (MQ=255) atatTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg > 1:231193/1‑71 (MQ=255) tttACGCACCAGCTCCGGGTGATCGTGGATCGCCTCa > 1:3258570/1‑37 (MQ=255) tACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGt > 1:43620/1‑71 (MQ=255) aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:3016528/1‑40 (MQ=255) aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:3304539/1‑40 (MQ=255) aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:836133/1‑40 (MQ=255) gCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGtttt < 1:344388/71‑1 (MQ=255) ccAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt < 1:2719848/50‑1 (MQ=255) cAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc > 1:11333/1‑71 (MQ=255) ttCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt < 1:3595851/71‑1 (MQ=255) ttCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt < 1:1907374/71‑1 (MQ=255) | CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAT > minE/1091100‑1091237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |