| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,138,595 | G→C | 7.5% | intergenic (+103/‑30) | ydjM → / → ydjN | predicted inner membrane protein regulated by LexA/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,138,595 | 0 | G | C | 7.5% | 203.0 / 5.6 | 67 | intergenic (+103/‑30) | ydjM/ydjN | predicted inner membrane protein regulated by LexA/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (23/37); new base C (2/3); total (25/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
TTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGT > minE/1138530‑1138662 | tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGa < 1:300637/71‑1 (MQ=255) gAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGaaa > 1:1141495/1‑70 (MQ=255) atatAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaata < 1:428306/70‑1 (MQ=255) tataAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat < 1:277985/71‑1 (MQ=255) tataAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat < 1:3479367/71‑1 (MQ=255) tataAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataa > 1:1110146/1‑70 (MQ=255) agagCCAGCTCAAAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAg < 1:2799240/70‑1 (MQ=255) gagCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGAt < 1:924899/71‑1 (MQ=255) ccAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGAt < 1:373254/68‑1 (MQ=255) cAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAgg < 1:1928560/71‑1 (MQ=255) cTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGaaa < 1:312233/55‑1 (MQ=255) cTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGaaa < 1:1857334/55‑1 (MQ=255) cTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGgag > 1:1118720/1‑70 (MQ=255) acaGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAAc < 1:1460782/70‑1 (MQ=255) acaGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACg < 1:3487009/71‑1 (MQ=255) ttCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAg < 1:3077127/45‑1 (MQ=255) ttCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGgag > 1:1046507/1‑63 (MQ=255) cTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGaa > 1:2003615/1‑45 (MQ=255) gTTAATCTTGCGCCAACACTATGACTGCTACGCAGTTATAGAAATAATAAGATCAGgag < 1:3441730/59‑1 (MQ=255) gTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGgag < 1:621010/59‑1 (MQ=255) gTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGgag < 1:1226451/59‑1 (MQ=255) gTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGgag < 1:1617352/59‑1 (MQ=255) ttAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat < 1:495581/47‑1 (MQ=255) ttAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat < 1:3724223/47‑1 (MQ=255) ttAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATc > 1:2954050/1‑53 (MQ=255) ttAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATc > 1:3340086/1‑53 (MQ=255) aaTCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACt < 1:1950645/70‑1 (MQ=255) aaTCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACt < 1:51457/70‑1 (MQ=255) gcgcCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCAt > 1:412218/1‑70 (MQ=255) cAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACg > 1:2052122/1‑50 (MQ=255) cAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAAtt < 1:406686/71‑1 (MQ=255) acacTATGACTGCTACGCAGTGATAGAAATAATAAGATCCGGAGAACGGGGATGAACTTTCCATTAATTGc > 1:1861929/1‑71 (MQ=255) acacTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGc > 1:874033/1‑71 (MQ=255) acacTATGACTGCTACGCAGTGATAGAAAAAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGc > 1:3161277/1‑71 (MQ=255) cacTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCg < 1:2297920/71‑1 (MQ=255) acTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCg > 1:1363814/1‑70 (MQ=255) cTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa < 1:3235844/71‑1 (MQ=255) cTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa < 1:269694/71‑1 (MQ=255) cTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa < 1:2566852/71‑1 (MQ=255) cTATCACTGCGTAGCAGTCATAGAAATAATAAGATCAGGAGACCGGGGATGAACTTTCCATTAATTGc > 1:1526580/1‑68 (MQ=25) cTATCACTGCGTAGCAGTCATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa > 1:2680169/1‑71 (MQ=37) tATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa > 1:3070607/1‑70 (MQ=255) tATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa > 1:296202/1‑70 (MQ=255) aTGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACgggg > 1:1017958/1‑46 (MQ=255) tGACTGCTACGCAGTTATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACa < 1:3713607/70‑1 (MQ=255) tGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTcc < 1:1434354/56‑1 (MQ=255) tGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAAc < 1:1480234/69‑1 (MQ=255) tGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAAc < 1:2387466/69‑1 (MQ=255) gACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATc > 1:1407466/1‑71 (MQ=255) gACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATc > 1:2438544/1‑71 (MQ=255) gACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATc > 1:259485/1‑71 (MQ=255) aCTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCg < 1:3343315/64‑1 (MQ=255) tGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGaa > 1:2223523/1‑64 (MQ=255) cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggt > 1:2875350/1‑71 (MQ=255) cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtgg > 1:3548486/1‑70 (MQ=255) tACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggtg > 1:2456339/1‑71 (MQ=255) tagCAGTCATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTc < 1:3485450/45‑1 (MQ=255) tagCAGTCATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTc < 1:86682/45‑1 (MQ=255) cGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggtg < 1:967652/69‑1 (MQ=255) cGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggtg < 1:1357581/69‑1 (MQ=255) cGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggtg < 1:3432979/69‑1 (MQ=255) cAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCg < 1:641395/71‑1 (MQ=255) cAGTCATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCg < 1:405275/71‑1 (MQ=255) aGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGt < 1:266011/71‑1 (MQ=255) aGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGt < 1:1667628/71‑1 (MQ=255) gTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACt < 1:3192717/39‑1 (MQ=255) gTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACt < 1:180999/39‑1 (MQ=255) | TTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGT > minE/1138530‑1138662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |