| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 145,530 | T→A | 60.4% | T233S (ACA→TCA) | sfsA ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 145,530 | 0 | T | A | 60.4% | 36.3 / 61.6 | 53 | T233S (ACA→TCA) | sfsA | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (14/7); new base A (11/21); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTT > minE/145466‑145595 | ggAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCg < 1:3387311/70‑1 (MQ=255) gAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGc < 1:2388815/71‑1 (MQ=255) aaaaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAAc < 1:12559/66‑1 (MQ=255) aaaaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCa < 1:56221/71‑1 (MQ=255) aaaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAg > 1:1168721/1‑71 (MQ=255) aaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGt > 1:1178899/1‑71 (MQ=255) aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGt > 1:2494882/1‑70 (MQ=255) aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGt > 1:787401/1‑70 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAAACGGCAGTGa < 1:1164141/69‑1 (MQ=255) aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtttt > 1:1568794/1‑71 (MQ=255) aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtttt > 1:1342106/1‑71 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTa < 1:3091679/70‑1 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTa < 1:592379/70‑1 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTa < 1:3148872/70‑1 (MQ=255) cgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAag < 1:1389476/71‑1 (MQ=255) cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGc > 1:791440/1‑70 (MQ=255) cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGc > 1:367214/1‑70 (MQ=255) cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGc > 1:1286266/1‑70 (MQ=255) cAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCt > 1:2865870/1‑71 (MQ=255) cAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCt < 1:2633421/71‑1 (MQ=255) cAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCt < 1:3586393/71‑1 (MQ=255) aGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCtt < 1:282085/71‑1 (MQ=255) aGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCt < 1:3143001/70‑1 (MQ=255) gAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCtt > 1:160892/1‑70 (MQ=255) gAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTc < 1:2557750/71‑1 (MQ=255) gAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTc < 1:2119233/71‑1 (MQ=255) aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCtt > 1:2758616/1‑69 (MQ=255) aaTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTg < 1:1062383/48‑1 (MQ=255) aaTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTg < 1:1448097/48‑1 (MQ=255) aTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag > 1:603165/1‑71 (MQ=255) aTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag > 1:3188302/1‑71 (MQ=255) gTAAATTAACCAGTTACTTACTTTACTACAATGaaa < 1:985213/36‑1 (MQ=255) gTAAATTAACCAGTTACTTACTTTACTACAATGaaa < 1:2070916/36‑1 (MQ=255) gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:3218697/1‑54 (MQ=255) gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:2723101/1‑54 (MQ=255) gTAAATTAACCAGTTACTTACTTTACCACAATGaaa < 1:2394187/36‑1 (MQ=38) tAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag > 1:2180224/1‑68 (MQ=255) aaaTTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagcag > 1:308459/1‑70 (MQ=255) ttAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATg > 1:2258330/1‑57 (MQ=255) aCCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc < 1:1201656/71‑1 (MQ=255) aCCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc < 1:1661106/71‑1 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCt < 1:2934103/71‑1 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCt > 1:2697571/1‑71 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCt > 1:1774255/1‑71 (MQ=255) cttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCtt < 1:182041/50‑1 (MQ=255) cttacttTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag > 1:3508384/1‑53 (MQ=255) cttacttTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTg < 1:972406/70‑1 (MQ=255) tactacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc > 1:857212/1‑68 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa < 1:1399836/71‑1 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa < 1:2482506/70‑1 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa < 1:1581161/70‑1 (MQ=255) tacAATGAAACCGGCAGTGATTTTTTAAGAGCCAt < 1:2869760/35‑1 (MQ=255) cAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAttt > 1:261225/1‑71 (MQ=255) cAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAtt > 1:3214907/1‑70 (MQ=255) cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt < 1:534119/69‑1 (MQ=255) cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt < 1:2900543/69‑1 (MQ=255) tAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag > 1:3613531/1‑36 (MQ=255) | GGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTT > minE/145466‑145595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |