| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,217,271 | G→C | 7.4% | R488G (CGT→GGT) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,217,271 | 0 | G | C | 7.4% | 290.4 / 8.6 | 93 | R488G (CGT→GGT) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base G (40/46); new base C (4/3); total (44/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAGCGCACGACCG > minE/1217206‑1217341 | cTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTc < 1:93012/70‑1 (MQ=255) aTCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATg > 1:1733303/1‑70 (MQ=255) tACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGt < 1:2949970/70‑1 (MQ=255) cTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAtt < 1:2233483/71‑1 (MQ=255) cacaGACCCCAGCGCTGGCCATTCAGGACGGACCATCTGATCGTAAATACGGTTCAATGAACGGTATTGCt > 1:3022640/1‑71 (MQ=255) cacaGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCt > 1:1846626/1‑71 (MQ=255) gAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACgg > 1:508518/1‑48 (MQ=255) aaCCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAtt > 1:37453/1‑63 (MQ=255) aaCCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAtt > 1:3165303/1‑63 (MQ=255) aaCCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACg > 1:473172/1‑71 (MQ=255) cccAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGt < 1:1234242/71‑1 (MQ=255) cccAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTACAATGAACGGTATTGCTGAACGGt < 1:601839/71‑1 (MQ=255) ccaaGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGt < 1:671283/68‑1 (MQ=255) cAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGc > 1:1488996/1‑71 (MQ=255) aGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGcc < 1:3682750/71‑1 (MQ=255) cgcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCtt < 1:2997621/71‑1 (MQ=255) cgcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCtt < 1:3383929/71‑1 (MQ=255) cgcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCtt < 1:2674076/71‑1 (MQ=255) gcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCttt < 1:1277120/71‑1 (MQ=255) gcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCttt < 1:2573834/71‑1 (MQ=255) gcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCttt < 1:2525099/71‑1 (MQ=255) gcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCtt < 1:422123/70‑1 (MQ=255) gcTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTAAACGGTGCCttt < 1:975254/71‑1 (MQ=255) cTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCttt > 1:3252067/1‑70 (MQ=255) gCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAAc < 1:1875480/58‑1 (MQ=255) gCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAAc < 1:1389926/58‑1 (MQ=255) gCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAAc < 1:538364/58‑1 (MQ=255) gCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAAc < 1:689608/58‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg < 1:913712/45‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg < 1:1256035/45‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg < 1:2074974/45‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg < 1:2837367/45‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCaa < 1:2386567/71‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCaa < 1:447574/71‑1 (MQ=255) ccATTCAGGACGTAACATCTGATCGTAAATACCGTTCATTGAACCGTAt < 1:1440334/49‑1 (MQ=25) cATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCaaa > 1:1840279/1‑71 (MQ=255) cATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCaaa > 1:1602468/1‑71 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:1095479/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:399305/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:3387007/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:2714180/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:2511312/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:128868/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:2442925/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:2238060/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAAc < 1:232740/71‑1 (MQ=255) aTTCAGGACGTAACATCTGATCGTAAATACCGTTCATTGAACCGTAttt > 1:157753/1‑48 (MQ=21) aTTCAGGACGTAACATCTGATCGTAAATACCGTTCATTGAACCGTAttt > 1:308581/1‑48 (MQ=21) aTTCAGGACGTAACATCTGATCGTAAATACCGTTCATTGAACCGTAttt > 1:1464015/1‑48 (MQ=21) ttCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTg < 1:1174931/51‑1 (MQ=255) ttCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACg > 1:3524872/1‑71 (MQ=255) ttCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACg > 1:3244449/1‑71 (MQ=255) tCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGt > 1:2326175/1‑71 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGt > 1:1192481/1‑42 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTa > 1:3605344/1‑43 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTa > 1:1305325/1‑43 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTa > 1:541436/1‑43 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTa > 1:363359/1‑43 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGt < 1:1296178/70‑1 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTc > 1:322684/1‑71 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTc > 1:2979640/1‑71 (MQ=255) cAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTc > 1:2397926/1‑71 (MQ=255) aGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCg > 1:206387/1‑71 (MQ=255) ggACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGaa > 1:3339293/1‑49 (MQ=255) ggACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGaa > 1:1299141/1‑49 (MQ=255) ggACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGaa > 1:369316/1‑49 (MQ=255) ggACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGaa > 1:2480014/1‑49 (MQ=255) gACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGt > 1:321914/1‑71 (MQ=255) gACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGt > 1:36580/1‑71 (MQ=255) aCGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGtt < 1:792738/71‑1 (MQ=255) cGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTc < 1:744942/71‑1 (MQ=255) cGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTc < 1:2245798/71‑1 (MQ=255) cGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTc < 1:2250137/71‑1 (MQ=255) gTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCa < 1:474075/71‑1 (MQ=255) aaCATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCAcc > 1:3574239/1‑71 (MQ=255) aaCATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCAcc > 1:829189/1‑71 (MQ=255) aCATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCAcc > 1:3498437/1‑70 (MQ=255) aTCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCcac < 1:3009375/71‑1 (MQ=255) aTCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCca > 1:1820744/1‑70 (MQ=255) cTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACaa > 1:2959347/1‑71 (MQ=255) cTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACaa > 1:3575417/1‑71 (MQ=255) tGATAGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAAc > 1:2866477/1‑71 (MQ=255) gATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCgg < 1:1600400/58‑1 (MQ=255) gATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCgg < 1:85796/58‑1 (MQ=255) gATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCgg < 1:2508784/58‑1 (MQ=255) tCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAAc > 1:1114001/1‑68 (MQ=255) cGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAgc > 1:420709/1‑71 (MQ=255) cGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAg > 1:1779686/1‑70 (MQ=255) agcaaTACCGTTCATTGAACCGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAgc < 1:1805887/67‑1 (MQ=37) agcaaTACCGTTCATTGAACCGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAgc < 1:1648218/67‑1 (MQ=37) tAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCa > 1:3410572/1‑67 (MQ=255) caaTACCGTTCATTGAACCGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCAc > 1:2196774/2‑57 (MQ=25) aaaTACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAGCGCa > 1:2159055/1‑71 (MQ=255) aTACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAGCGCACg < 1:1527392/71‑1 (MQ=255) ggTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAGCGCACGACCg < 1:2649985/71‑1 (MQ=255) | CTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTATTGCTGAACGGTGCCTTTACCAAACGTCGGTTCACCCACAACCAGCGCACGACCG > minE/1217206‑1217341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |