| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,237,336 | C→A | 6.9% | R395R (CGG→CGT) | edd ← | 6‑phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,237,336 | 0 | C | A | 6.9% | 281.5 / 3.7 | 84 | R395R (CGG→CGT) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (39/39); new base A (3/3); total (42/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGC > minE/1237266‑1237401 | caccaTGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCTAGTGATTTTTCCGCCCCTTcc > 1:2823788/1‑71 (MQ=255) accaTGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTccc > 1:466266/1‑71 (MQ=255) caTGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGc > 1:688716/1‑71 (MQ=255) caTGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGc > 1:1984271/1‑71 (MQ=255) tgaGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtc < 1:2514781/71‑1 (MQ=255) tgaGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtc < 1:1414921/71‑1 (MQ=255) agagaAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtcc < 1:999423/70‑1 (MQ=255) aaaGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtc < 1:1033728/65‑1 (MQ=255) aaGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTccc > 1:2154920/1‑57 (MQ=255) aaGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTccc > 1:3097116/1‑57 (MQ=255) aGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCAc < 1:2308415/71‑1 (MQ=255) aGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCAc > 1:2948898/1‑71 (MQ=255) ggTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccagt < 1:366194/66‑1 (MQ=255) gttgttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCa > 1:1579423/1‑71 (MQ=255) ttgttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCat < 1:2029205/71‑1 (MQ=255) gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCatt < 1:1249041/70‑1 (MQ=255) gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCACGCCAGTCCAGTTCAc > 1:3731427/1‑66 (MQ=255) gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCACGCCAGTCCAGTTCAc > 1:1652616/1‑66 (MQ=255) attCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCACGCCAGTCCAGTTCAc > 1:3362817/2‑66 (MQ=255) ttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCattat < 1:2193351/71‑1 (MQ=255) cGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCattatt > 1:1314794/1‑70 (MQ=255) cGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCattatt > 1:2461531/1‑70 (MQ=255) gtaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTc < 1:1441107/68‑1 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:3340608/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:3344950/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:3431853/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:3457617/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:2681757/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:417922/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:2521589/1‑46 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGcc < 1:3243630/50‑1 (MQ=255) gaaggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCACTTCCCGCCAGTCCAGTTCACAattat > 1:715132/1‑68 (MQ=255) aggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGc > 1:2199466/1‑71 (MQ=255) ggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGc > 1:2579679/1‑70 (MQ=255) aagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCa > 1:2182376/1‑70 (MQ=255) aagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt > 1:3044979/1‑71 (MQ=255) aagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:2635462/71‑1 (MQ=255) agCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATg < 1:1836239/71‑1 (MQ=255) gCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATgg < 1:2336635/71‑1 (MQ=255) gCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATgg > 1:3302944/1‑71 (MQ=255) gATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccag < 1:1200541/48‑1 (MQ=255) gATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGtt > 1:458273/1‑71 (MQ=255) tCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCTTGGTTCa < 1:3582607/71‑1 (MQ=255) tCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa < 1:1718725/71‑1 (MQ=255) tCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa < 1:1089694/71‑1 (MQ=255) cacaTTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc > 1:792630/1‑69 (MQ=255) acaTTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa < 1:3504351/70‑1 (MQ=255) caTTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAgg > 1:2521633/1‑71 (MQ=255) gCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGta > 1:1924082/1‑70 (MQ=255) cTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCa > 1:81502/1‑42 (MQ=255) cTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCa > 1:495992/1‑42 (MQ=255) cTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtat < 1:3335312/70‑1 (MQ=255) gTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa > 1:142090/1‑61 (MQ=255) gTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAAc > 1:1259517/1‑71 (MQ=255) gTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAAc > 1:127340/1‑71 (MQ=255) tCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:417274/54‑1 (MQ=255) tCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:2540341/54‑1 (MQ=255) tCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:639425/54‑1 (MQ=255) tCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAAc > 1:2259768/1‑70 (MQ=255) gAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACga < 1:2599606/70‑1 (MQ=255) gAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTAAGCCATGGTTCAAGGGTATAACga < 1:1962653/70‑1 (MQ=255) aGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga < 1:3424941/71‑1 (MQ=255) aGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga < 1:330235/71‑1 (MQ=255) aGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga < 1:1388142/71‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCa < 1:1830897/38‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCa < 1:1029398/38‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCa < 1:2887866/38‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac < 1:1082549/71‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac < 1:2608550/71‑1 (MQ=255) gTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac < 1:998473/71‑1 (MQ=255) aTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagacag < 1:2005944/70‑1 (MQ=255) tttttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa < 1:2905954/52‑1 (MQ=255) ttttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagacag > 1:3472023/1‑68 (MQ=255) ttttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagacag > 1:1668406/1‑68 (MQ=255) tttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATgg > 1:2984062/1‑46 (MQ=255) tttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAg > 1:2694221/1‑52 (MQ=255) ttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCa > 1:1767186/1‑70 (MQ=255) cGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAAc < 1:2090558/71‑1 (MQ=255) cGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAAc < 1:2605200/71‑1 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc > 1:690126/1‑43 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc > 1:243553/1‑43 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc > 1:2389516/1‑43 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc > 1:2960466/1‑43 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACg > 1:163093/1‑55 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCaaaa > 1:2233887/1‑68 (MQ=255) ccccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCt < 1:3517602/71‑1 (MQ=255) cccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAGCCTg > 1:1654202/1‑71 (MQ=255) cccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTg > 1:1143629/1‑71 (MQ=255) ccTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGc > 1:3080421/1‑71 (MQ=255) cTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGc > 1:2617856/1‑70 (MQ=255) cTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGc > 1:233988/1‑70 (MQ=255) cTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGc > 1:487431/1‑70 (MQ=255) cTTCACGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:2506093/57‑1 (MQ=255) cTTCACGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:722335/57‑1 (MQ=255) cTTCACGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:2538471/57‑1 (MQ=255) ccGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtat < 1:2478103/44‑1 (MQ=255) ccGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtat < 1:784267/44‑1 (MQ=255) ccGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtat < 1:489064/44‑1 (MQ=255) ccGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtat < 1:3058395/44‑1 (MQ=255) | CACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGC > minE/1237266‑1237401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |