| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,292,502 | G→A | 7.3% | T219M (ACG→ATG) | yeeO ← | predicted multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,292,502 | 0 | G | A | 7.3% | 216.7 / 4.8 | 68 | T219M (ACG→ATG) | yeeO | predicted multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (29/34); new base A (3/2); total (32/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAAT > minE/1292434‑1292572 | tATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTc < 1:1979358/71‑1 (MQ=255) ccTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTc > 1:2118557/1‑71 (MQ=255) cTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTc > 1:891679/1‑70 (MQ=255) gCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGCTAACTGAGTACCGTCAGCTc > 1:3705217/1‑68 (MQ=255) gCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTc > 1:1091388/1‑68 (MQ=255) accacGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt < 1:2114775/71‑1 (MQ=255) accacGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt < 1:1258798/71‑1 (MQ=255) ccacGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt < 1:3102917/70‑1 (MQ=255) cacGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt > 1:1314528/1‑69 (MQ=255) acGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAg > 1:2163651/1‑71 (MQ=255) cGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCAAGATAAGt > 1:479809/1‑71 (MQ=255) gAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGt > 1:1034213/1‑70 (MQ=255) aaGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTc > 1:1084408/1‑70 (MQ=255) gTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAAc < 1:1470061/71‑1 (MQ=255) ccGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCg < 1:309810/46‑1 (MQ=255) ccGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAg < 1:901180/71‑1 (MQ=255) cGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGa < 1:983536/56‑1 (MQ=255) cGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATa < 1:2456365/58‑1 (MQ=255) cGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATa < 1:723331/58‑1 (MQ=255) gCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTg < 1:138232/71‑1 (MQ=255) ccAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAATTCAACGCCAGTGCtt > 1:1454218/1‑70 (MQ=255) ccAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt > 1:318667/1‑70 (MQ=255) ccAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt > 1:3426678/1‑70 (MQ=255) aaTAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCaa > 1:3199500/1‑57 (MQ=255) tAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACt < 1:273682/71‑1 (MQ=255) agagTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACtt < 1:2057985/70‑1 (MQ=255) agagTGATGGCAGCTGCTGGATAACTGAGTACCGTAAGCTCCAGATAAGTCAAc > 1:3348300/1‑54 (MQ=255) gagTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt < 1:1906704/45‑1 (MQ=255) gagTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt < 1:2934683/45‑1 (MQ=255) gagTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCt < 1:10183/71‑1 (MQ=255) tgttGGCAGCTGCTGGATAACTGAGTACCATCAGCTCCAGATAAGTCAACGCCAGTGCTTTAAc < 1:1655978/61‑1 (MQ=255) tGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTc < 1:1001788/71‑1 (MQ=255) tGATGGCAGCTGCTGGATAACTGAGTACCATCAGCTCCAGATAAGTCAACGCCAGTGCTTTAAc < 1:1663211/64‑1 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTc < 1:539189/45‑1 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAg > 1:1695584/1‑53 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAg > 1:1185273/1‑53 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCATCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACtt > 1:3353226/1‑64 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCATCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACtt > 1:3500911/1‑64 (MQ=255) aTGGCAGCTGCTGGATAACTGAGTACCATCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACtt > 1:2866786/1‑64 (MQ=255) tGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCttt < 1:144710/58‑1 (MQ=255) tGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCttt < 1:1871339/58‑1 (MQ=255) tGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCttt < 1:2198012/58‑1 (MQ=255) tGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCttt < 1:6293/58‑1 (MQ=255) gctgctGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCa > 1:2552099/1‑69 (MQ=255) gctgctGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCAt > 1:481428/1‑70 (MQ=255) gctgctGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCAGTCGTGGCAt > 1:1551821/1‑70 (MQ=255) tgctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCAc > 1:2230481/1‑71 (MQ=255) tgctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCAAGTGCTTTAACTTCTGTCGTGGCATCAc > 1:3220444/1‑71 (MQ=255) gctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCaa > 1:3612307/1‑38 (MQ=255) gctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCaa > 1:3114295/1‑38 (MQ=255) ctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCAcc > 1:694806/1‑70 (MQ=255) gATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCcgc < 1:3603417/70‑1 (MQ=255) aTAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTg > 1:203542/1‑58 (MQ=255) tAACTGAGTACCGTCAGCTCAAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGcga > 1:1630334/1‑70 (MQ=255) tAACTGAGTACCGTCAGCACCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCg > 1:3689731/1‑55 (MQ=255) aaCTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTa < 1:3670803/44‑1 (MQ=255) aaCTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTa < 1:3241638/44‑1 (MQ=255) aaCTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACtt < 1:1276192/48‑1 (MQ=255) aaCTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCcgc > 1:3520485/1‑67 (MQ=255) aCTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGcgacga < 1:281971/71‑1 (MQ=255) cTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGaa < 1:1403084/71‑1 (MQ=255) cTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGaa < 1:2295069/71‑1 (MQ=255) cTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCATTAACt > 1:1287034/1‑45 (MQ=255) aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAAt < 1:1215818/70‑1 (MQ=255) aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAAt < 1:2901828/70‑1 (MQ=255) aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATc < 1:13548/71‑1 (MQ=255) cGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCaataa < 1:1547008/71‑1 (MQ=255) cGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCaataa < 1:880061/71‑1 (MQ=255) cGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGACGTGGCATCACCCGCGACGAAATCaataa < 1:662949/71‑1 (MQ=255) gTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCaataat > 1:2803880/1‑71 (MQ=255) gTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCaataa > 1:1655522/1‑70 (MQ=255) | TATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAAT > minE/1292434‑1292572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |