| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,435,102 | C→T | 16.7% | G356S (GGC→AGC) | yojI ← | fused predicted multidrug transport subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,435,102 | 0 | C | T | 16.7% | 145.2 / 21.0 | 60 | G356S (GGC→AGC) | yojI | fused predicted multidrug transport subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (21/29); new base T (7/3); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGA > minE/1435033‑1435172 | cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgcc > 1:1036016/1‑70 (MQ=255)cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgcc > 1:3404351/1‑70 (MQ=255)cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgcc > 1:3649711/1‑70 (MQ=255) cgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAAt < 1:3561882/70‑1 (MQ=255) cgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATc < 1:2767044/71‑1 (MQ=255) gcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAAt > 1:338881/1‑69 (MQ=255) tgtgGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaaa > 1:747642/1‑71 (MQ=255) tgtgGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaa < 1:61473/70‑1 (MQ=255) cTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcagc < 1:549607/70‑1 (MQ=255) aTACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCt > 1:1089174/1‑71 (MQ=255) aaGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc < 1:2826612/71‑1 (MQ=255) gCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCAc < 1:1085373/71‑1 (MQ=255) cccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCTAATCAGAAAcagc > 1:3130971/1‑60 (MQ=255) cccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACg < 1:2586487/71‑1 (MQ=255) ccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACg < 1:3149666/70‑1 (MQ=255) ccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGt > 1:1084318/1‑71 (MQ=255) cGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaaa > 1:3181885/1‑54 (MQ=255) tCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGa < 1:2796215/50‑1 (MQ=255) tCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGa < 1:1964773/50‑1 (MQ=255) caacaTCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCCGCTCGCCACGTTTGATg > 1:465055/1‑70 (MQ=255) tCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGt < 1:2830805/59‑1 (MQ=255) gCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGAtt < 1:461777/71‑1 (MQ=255) gCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGAt > 1:178371/1‑70 (MQ=255) ccAGCGTCGATTTTCCGCTACCGTTGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTa < 1:810776/71‑1 (MQ=255) cAGCGTCGATTTTCCGCTACCGTTGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGACATTaa < 1:3513150/71‑1 (MQ=255) cAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTaa < 1:1894221/71‑1 (MQ=255) aGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCTCCACGTTTGATGTTGAGATTAAt < 1:841328/71‑1 (MQ=255) aGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAAt < 1:1709584/71‑1 (MQ=255) cGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCg < 1:1249689/71‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCAc < 1:571377/45‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCAc < 1:1451508/45‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCAc < 1:2595775/45‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCAc < 1:214920/45‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTg < 1:3476574/50‑1 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGAcc > 1:807799/1‑70 (MQ=255) tttCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAAc > 1:2485076/1‑71 (MQ=255) tCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACg < 1:3169787/70‑1 (MQ=255) tCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACg > 1:1648135/1‑70 (MQ=255) ccGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACgg < 1:3700077/70‑1 (MQ=255) cGCTACCGTTGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa < 1:1238948/71‑1 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGTCACGTTTGATGGTGAGATTAATCGGACCAACGGaaa < 1:3063703/71‑1 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaaa < 1:3528250/71‑1 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa > 1:1372251/1‑70 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa > 1:1774881/1‑70 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGAGAGATTAATCGGACCAACGGaa > 1:1555722/1‑70 (MQ=255) cTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaaaa > 1:86780/1‑71 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa < 1:1051127/68‑1 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa < 1:2315926/68‑1 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa < 1:736748/68‑1 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGaa < 1:2013365/68‑1 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAc > 1:192391/1‑71 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAc > 1:1516473/1‑71 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAc > 1:838579/1‑71 (MQ=255) tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAc > 1:3034124/1‑71 (MQ=255) cGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTa > 1:3096452/1‑50 (MQ=255) gTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGt > 1:3040443/1‑42 (MQ=255) gTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGt > 1:1023703/1‑42 (MQ=255) ttGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:3254449/1‑70 (MQ=255) ttGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:2811549/1‑70 (MQ=255) ttGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:588868/1‑70 (MQ=255) ttGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:3665684/1‑70 (MQ=255) ttGCCGCTAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:3670112/1‑70 (MQ=255) ttGCCGCTAATCAGAAACACCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGt > 1:335216/1‑70 (MQ=255) gccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTAt > 1:1251548/1‑71 (MQ=255) ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTAt < 1:2681964/70‑1 (MQ=255) ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTAt < 1:2136535/70‑1 (MQ=255) ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTAt < 1:2019476/70‑1 (MQ=255) ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTAt < 1:2733629/70‑1 (MQ=255) ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATc > 1:966650/1‑71 (MQ=255) ctccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATcc < 1:3124688/69‑1 (MQ=255) gccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt < 1:1273041/71‑1 (MQ=255) gccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt < 1:131240/71‑1 (MQ=255) gccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt > 1:2969415/1‑71 (MQ=255) ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATcc > 1:657274/1‑69 (MQ=255) ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt > 1:1646771/1‑70 (MQ=255) ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt > 1:2775735/1‑70 (MQ=255) ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCt > 1:1891139/1‑70 (MQ=255) ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTg < 1:3248378/71‑1 (MQ=255) cAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGa > 1:3574950/1‑71 (MQ=255) | CGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGA > minE/1435033‑1435172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |