| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,464,342 | A→T | 10.5% | intergenic (‑146/+3) | yfaA ← / ← gyrA | hypothetical protein/DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,464,342 | 0 | A | T | 10.5% | 212.3 / 14.9 | 76 | intergenic (‑146/+3) | yfaA/gyrA | hypothetical protein/DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (35/33); new base T (3/5); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
CCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCA > minE/1464273‑1464411 | cccAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaaa < 1:2010866/71‑1 (MQ=255) cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAat < 1:2426866/71‑1 (MQ=255) cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAat < 1:1554109/71‑1 (MQ=255) cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAat < 1:2879750/71‑1 (MQ=255) aGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAat > 1:916927/1‑70 (MQ=255) aGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGAATAatt > 1:2142907/1‑71 (MQ=255) tttGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAatta > 1:1291009/1‑68 (MQ=255) tttGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAatta > 1:3579619/1‑68 (MQ=255) tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttctt > 1:236937/1‑71 (MQ=255) tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttct > 1:1754505/1‑70 (MQ=255) tGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTgg > 1:1961720/1‑71 (MQ=255) ggACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGc > 1:1843994/1‑71 (MQ=255) aCTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCtc < 1:2356738/71‑1 (MQ=255) aCTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCt > 1:1365599/1‑70 (MQ=255) aCTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCt > 1:785882/1‑70 (MQ=255) cTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGAATAATTTtacttct < 1:2019368/62‑1 (MQ=37) ttttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCtcgt > 1:3335125/1‑71 (MQ=255) tttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaaaa < 1:3724257/50‑1 (MQ=255) tttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGAATAATTTtacttcttc > 1:586902/1‑62 (MQ=25) tttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGAATAATTTtacttcttc > 1:2550090/1‑62 (MQ=25) ttcaattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttct > 1:2571184/1‑56 (MQ=255) caattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTcgtcgt < 1:968149/70‑1 (MQ=255) aattcaaACAAGGGAGATATCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCgtcgtc > 1:2302747/1‑70 (MQ=255) ttcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaa < 1:1309078/42‑1 (MQ=255) ttcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaa < 1:3449656/42‑1 (MQ=255) ttcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaa < 1:3519785/42‑1 (MQ=255) ttcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaa < 1:2058353/42‑1 (MQ=255) ttcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAAc > 1:3003284/1‑71 (MQ=255) caaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg < 1:705827/70‑1 (MQ=255) caaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg < 1:2437931/70‑1 (MQ=255) caaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg < 1:2523440/70‑1 (MQ=255) aaaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGt < 1:569471/70‑1 (MQ=255) aaaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGt < 1:1325209/70‑1 (MQ=255) aaaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTc < 1:2034218/71‑1 (MQ=255) aaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTc > 1:2379456/1‑70 (MQ=255) aCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCa < 1:2707681/71‑1 (MQ=255) cAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTCCCTCGTCGTCAACGTCCAc > 1:363026/1‑71 (MQ=255) aaGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAatta < 1:1384536/40‑1 (MQ=255) aGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACtt < 1:3353781/71‑1 (MQ=255) gggAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTc < 1:2409926/71‑1 (MQ=255) gggAGATAGCTCCCTTTTGGCATGAAGAAGAATAattt < 1:444172/38‑2 (MQ=37) gggAGATAGCTCCCTTTTGGCATGAAGAAGAATAattt < 1:976010/38‑2 (MQ=37) gggAGATAGCTCCCTTTTGGCATGAAGAAGAATAattt < 1:1146919/38‑2 (MQ=37) gggAGATAGCTCCCTTTTGGAATGAAGAAGTAAAATTATtcttcttct < 1:2533143/48‑1 (MQ=255) ggAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTc < 1:2712102/70‑1 (MQ=255) gagaTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTGTTCTTCTgg > 1:3379255/1‑48 (MQ=255) gagaTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCg < 1:1913088/71‑1 (MQ=255) gagaTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCg < 1:667500/71‑1 (MQ=255) aTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCgtcgtct < 1:1409857/55‑2 (MQ=255) aGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAAc > 1:1152169/1‑55 (MQ=255) aGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGc > 1:1894605/1‑70 (MQ=255) aGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGc > 1:3675664/1‑70 (MQ=255) aGCTCCCTTTTGGCATGAAGAAGAAAAATTATTCTTCTTCTGGCTCGTCGTCa > 1:1371293/1‑53 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGGTCGTCGTCAACGTCCACTTCCGGAGCGAtt < 1:376067/69‑1 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg < 1:1670441/51‑1 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg < 1:1477909/51‑1 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGAttt < 1:3027424/70‑1 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGAttt < 1:430279/70‑1 (MQ=255) ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGAtt > 1:1338914/1‑69 (MQ=255) cTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCa < 1:1200272/71‑1 (MQ=255) ttttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCAt > 1:2572823/1‑71 (MQ=255) tttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCtcgtc > 1:2404320/1‑41 (MQ=255) tttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc > 1:3117122/1‑71 (MQ=255) tttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc > 1:2017164/1‑71 (MQ=255) tttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc > 1:2643264/1‑71 (MQ=255) tttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc > 1:3528035/1‑71 (MQ=255) ttGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCAt < 1:3624061/69‑1 (MQ=255) tGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCg < 1:1084567/70‑1 (MQ=255) ggCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGt < 1:2442992/70‑1 (MQ=255) ggCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGt < 1:3424502/70‑1 (MQ=255) gaagaaGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTcc > 1:1836518/1‑67 (MQ=255) gaagaaGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTcc > 1:3300689/1‑67 (MQ=255) gaagaaGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTcc > 1:1025831/1‑67 (MQ=255) gaagaaGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTc > 1:3498799/1‑66 (MQ=255) aagaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTcc > 1:3308644/1‑66 (MQ=255) aagaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTcc > 1:2984661/1‑66 (MQ=255) aagaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCtt < 1:2664409/70‑1 (MQ=255) aagaagAATAATTTTACTTCTTCTGGCTCGTCGTCAACg < 1:2219/39‑1 (MQ=14) agaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCAc < 1:1110255/43‑1 (MQ=255) agaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCAc < 1:257543/43‑1 (MQ=255) agaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTcc > 1:1450704/1‑71 (MQ=255) agaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTcc > 1:306506/1‑71 (MQ=255) aaaaTTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCa > 1:1557584/1‑71 (MQ=255) | CCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCA > minE/1464273‑1464411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |