Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,585,153 G→T 9.5% Q211H (CAG→CAT cysZ → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,585,1530GT 9.5% 346.3 / 13.3 105Q211H (CAG→CATcysZpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (47/48);  new base T (6/4);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 106 total aligned reads displayed.

CGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTT  >  minE/1585084‑1585223
                                                                     |                                                                      
cGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGt                                                                       >  1:2585901/1‑71 (MQ=255)
  aTAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGttt                                                                     >  1:922554/1‑71 (MQ=255)
  aTAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGttt                                                                     >  1:468052/1‑71 (MQ=255)
   tAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTg                                                                    <  1:1361168/71‑1 (MQ=255)
   tAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTg                                                                    <  1:1690965/71‑1 (MQ=255)
    aaCCACAAAGTGCCTTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   >  1:3075738/1‑71 (MQ=255)
    aaCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   >  1:3015250/1‑71 (MQ=255)
    aaCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   >  1:2128323/1‑71 (MQ=255)
     aCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   <  1:1059890/70‑1 (MQ=255)
     aCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   <  1:642841/70‑1 (MQ=255)
     aCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGt                                                                  >  1:606961/1‑71 (MQ=255)
     aCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAAACTGCATATTGGt                                                                  <  1:1405488/71‑1 (MQ=38)
     aCCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAAACTGCATATTGGt                                                                  <  1:3134222/71‑1 (MQ=38)
      ccACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTg                                                                 >  1:1989334/1‑71 (MQ=255)
        acaAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCt                                                               <  1:692628/71‑1 (MQ=255)
         caAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCtt                                                              >  1:2458363/1‑71 (MQ=255)
         caAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCtt                                                              >  1:369205/1‑71 (MQ=255)
             gTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAAc                                                          <  1:3532075/71‑1 (MQ=255)
              tGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGtt                                                                      <  1:1410530/58‑1 (MQ=255)
              tGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAAc                                                          >  1:1648869/1‑70 (MQ=255)
                     tAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTg                                                   <  1:2749584/70‑1 (MQ=255)
                     tAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTg                                                   <  1:771267/70‑1 (MQ=255)
                     tAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTg                                                   <  1:1870844/70‑1 (MQ=255)
                     tAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGt                                                  >  1:2071737/1‑71 (MQ=255)
                     tAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAAACTGCATATTGGt                                                                  <  1:2516620/55‑1 (MQ=37)
                      aaaGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCa                                                        <  1:1516927/64‑1 (MQ=255)
                      aaaGAGATGCGCACCGCCCTGCCCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCa                                                        <  1:3550010/64‑1 (MQ=255)
                       aaGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGtt                                                                      >  1:2775382/1‑49 (MQ=255)
                        agagATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGc                                                      <  1:1145146/64‑1 (MQ=255)
                         gagaTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTAc                                              <  1:310324/71‑1 (MQ=255)
                         gagaTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTAc                                              <  1:3097329/71‑1 (MQ=255)
                         gagaTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTAc                                              <  1:1496044/71‑1 (MQ=255)
                         gagaTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTAc                                              >  1:3605940/1‑71 (MQ=255)
                           gaTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACga                                            <  1:2976869/71‑1 (MQ=255)
                            aTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgat                                           >  1:470579/1‑71 (MQ=255)
                            aTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgat                                           >  1:287573/1‑71 (MQ=255)
                             tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgatg                                          <  1:2701685/71‑1 (MQ=255)
                             tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgatg                                          <  1:862798/71‑1 (MQ=255)
                             tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgat                                           <  1:1910586/70‑1 (MQ=255)
                             tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgat                                           >  1:3409216/1‑70 (MQ=255)
                              gcgcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAAcc                                                         <  1:1158136/55‑1 (MQ=255)
                              gcgcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgatga                                         >  1:3675885/1‑71 (MQ=255)
                               cgcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgatgat                                        <  1:1910654/71‑1 (MQ=255)
                                gcACCGCCTTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAAcc                                                         >  1:1468432/1‑53 (MQ=255)
                                gcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTgg                                                                   <  1:2824331/43‑1 (MQ=255)
                                gcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATc                                       <  1:2478876/71‑1 (MQ=255)
                                gcACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATc                                       >  1:2841569/1‑71 (MQ=255)
                                 cACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATcc                                      <  1:3226478/71‑1 (MQ=255)
                                 cACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATcc                                      <  1:350449/71‑1 (MQ=255)
                                     gCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCgct                                  <  1:387130/71‑1 (MQ=255)
                                         tGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTaa                                                           >  1:3332452/1‑42 (MQ=255)
                                          gcgcACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgat                                           >  1:1387511/1‑57 (MQ=255)
                                             cacaCGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCgct                                  <  1:1663504/63‑1 (MQ=255)
                                               cacGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTg                        <  1:3313285/71‑1 (MQ=255)
                                               cacGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTg                        <  1:2658632/71‑1 (MQ=255)
                                                acGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTa                             <  1:2950743/65‑1 (MQ=255)
                                                acGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTa                             <  1:3441381/65‑1 (MQ=255)
                                                acGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGt                       <  1:206833/71‑1 (MQ=255)
                                                 cGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAg                                                       >  1:3509447/1‑38 (MQ=255)
                                                 cGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAg                                                       >  1:3243309/1‑38 (MQ=255)
                                                 cGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAg                                                       >  1:2601287/1‑38 (MQ=255)
                                                 cGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAg                                                       >  1:403105/1‑38 (MQ=255)
                                                   cAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCtt                              <  1:1928908/61‑1 (MQ=255)
                                                    aaaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCgctgc                                >  1:653350/1‑58 (MQ=255)
                                                    aaaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTtcat                   >  1:325199/1‑71 (MQ=255)
                                                    aaaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTtcat                   >  1:2508038/1‑71 (MQ=255)
                                                    aaaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCCGCTTAATCTGTtcat                   >  1:3548754/1‑71 (MQ=255)
                                                     aaaTCACCAATATGCAGTTTGGTGCTTTAACGAGCCTGTTTACGATGATCCCGCTACTTAATCTGTtcat                   <  1:1968289/70‑1 (MQ=255)
                                                      aaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTg                                                   <  1:1865766/37‑1 (MQ=255)
                                                      aaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTTATCTGTtcatca                 <  1:1775293/71‑1 (MQ=255)
                                                      aaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTtcatca                 <  1:1950544/71‑1 (MQ=255)
                                                       aTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGtt                                                 >  1:1483373/1‑38 (MQ=255)
                                                       aTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACAATGATCCCGCTGCTTAATCTGTTcatcat                <  1:2560350/71‑1 (MQ=255)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:595462/1‑71 (MQ=38)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:886661/1‑71 (MQ=38)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:1378994/1‑71 (MQ=38)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:1443657/1‑71 (MQ=38)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:2290335/1‑71 (MQ=38)
                                                         cACCAAACTGCATATTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGc              >  1:2769645/1‑71 (MQ=38)
                                                            cAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCg           >  1:614919/1‑71 (MQ=255)
                                                             aaTATGCAGTTTGGTGCTTTAACCAGCCTGTTTACgatg                                          <  1:38516/39‑1 (MQ=255)
                                                              atatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGtt         <  1:1473135/71‑1 (MQ=255)
                                                              atatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGtt         >  1:3697629/1‑71 (MQ=255)
                                                              atatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGtt         >  1:2963454/1‑71 (MQ=255)
                                                               tatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTg        <  1:511916/71‑1 (MQ=255)
                                                               tatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTg        <  1:596782/71‑1 (MQ=255)
                                                               tatGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTg        <  1:1106105/71‑1 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTcatcat                >  1:3213041/1‑61 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATg               >  1:3294260/1‑62 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATg               >  1:689045/1‑62 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATg               >  1:2460420/1‑62 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATg               >  1:3645656/1‑62 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGcc      >  1:2462772/1‑71 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGcc      >  1:2973704/1‑71 (MQ=255)
                                                                 tGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGcc      >  1:1942042/1‑71 (MQ=255)
                                                                  gCAGTTTGGTGCTTTATCCAGCCTGTTTACGATGATCCCGCTGCTTAATCTg                        <  1:62408/52‑1 (MQ=255)
                                                                  gCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTg                        <  1:704117/52‑1 (MQ=255)
                                                                    aGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGtt   >  1:723449/1‑71 (MQ=255)
                                                                    aGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGtt   >  1:1544499/1‑71 (MQ=255)
                                                                     gTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGttt  <  1:1010250/71‑1 (MQ=255)
                                                                     |                                                                      
CGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTT  >  minE/1585084‑1585223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: