| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,666,801 | T→A | 12.3% | intergenic (‑45/‑117) | guaB ← / → xseA | IMP dehydrogenase/exonuclease VII, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,666,801 | 0 | T | A | 12.3% | 152.3 / 13.0 | 57 | intergenic (‑45/‑117) | guaB/xseA | IMP dehydrogenase/exonuclease VII, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (19/31); new base A (2/5); total (21/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GAGCTTCTTTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGG > minE/1666732‑1666865 | gagCTTCTTTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCAtt < 1:184881/71‑1 (MQ=255) agCTTCTTTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATta < 1:1638960/71‑1 (MQ=255) agCTTCTTTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGAGGCATta < 1:2934472/71‑1 (MQ=255) cttcttTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATtat < 1:2037513/70‑1 (MQ=255) aGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGc < 1:164628/70‑1 (MQ=255) cGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTa < 1:807919/71‑1 (MQ=255) aTACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAAc > 1:614868/1‑71 (MQ=255) aTACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAAc > 1:682474/1‑71 (MQ=255) gCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTg > 1:831446/1‑70 (MQ=255) gCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTg > 1:2043424/1‑70 (MQ=255) gCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGc < 1:3657760/71‑1 (MQ=255) cATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAAcc < 1:1654117/64‑1 (MQ=255) cATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAAcc < 1:3658454/64‑1 (MQ=255) cATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCa < 1:1532550/71‑1 (MQ=255) aTGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCg < 1:2399114/64‑1 (MQ=255) aTGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCa < 1:1331108/70‑1 (MQ=255) ttggCAATATCTCGACCAGAGTTGTTAATAAATATTGCCGCGGTATTATACAGAGCGTAACCg < 1:1582001/61‑1 (MQ=255) tGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGa > 1:452251/1‑64 (MQ=255) tGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATc < 1:2799161/71‑1 (MQ=255) tGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATc > 1:1964503/1‑71 (MQ=255) tGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATc > 1:1968858/1‑71 (MQ=255) tGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATc < 1:850906/71‑1 (MQ=255) atCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCtt > 1:1487736/1‑71 (MQ=255) atCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCtt > 1:1440857/1‑71 (MQ=255) tctcGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCttt > 1:1289851/1‑71 (MQ=255) gACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCtttt < 1:2408549/68‑1 (MQ=255) gACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCttt < 1:2131018/67‑1 (MQ=255) gACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCttt < 1:795423/67‑1 (MQ=255) aCCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGc > 1:2488014/1‑70 (MQ=255) ccAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCa > 1:1623031/1‑70 (MQ=255) gactGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCaaaaa < 1:393692/68‑1 (MQ=255) tGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAt < 1:2165728/70‑1 (MQ=255) gTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtgc < 1:2391967/70‑1 (MQ=255) gTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtgc < 1:805972/70‑1 (MQ=255) taataaATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtgcttg > 1:2397126/1‑71 (MQ=255) aataaataTTGCCGCGGCATTATACATAGCGTAACCGAtt < 1:1553172/40‑1 (MQ=255) taaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTa < 1:266661/71‑1 (MQ=255) taaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTa > 1:485256/1‑71 (MQ=255) taaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTa > 1:3728598/1‑71 (MQ=255) aaataTTGCCGCGGCATTATACAGAGCTTAACCGATTGc < 1:1770645/39‑1 (MQ=255) aaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGc < 1:3277194/39‑1 (MQ=255) atatTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATcc > 1:3151975/1‑71 (MQ=255) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATc < 1:1854972/40‑1 (MQ=37) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATc < 1:1349962/40‑1 (MQ=37) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATc < 1:3319228/40‑1 (MQ=37) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATc < 1:699419/40‑1 (MQ=37) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATc < 1:2830050/40‑1 (MQ=37) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATCTACCCCTTTTTg > 1:239947/1‑52 (MQ=38) ataaTGCCGCGGCAATATACAGAGCGTAACCGATTGCATCTACCCCTTTTTg > 1:588050/1‑52 (MQ=38) ttGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCg < 1:1767571/71‑1 (MQ=255) gCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCg > 1:2075715/1‑69 (MQ=255) ccGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCttt < 1:3013768/43‑1 (MQ=255) ccGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtg < 1:975878/55‑1 (MQ=255) cgcgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtgcttgc < 1:2001755/59‑1 (MQ=255) cgcgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtg < 1:1022790/54‑1 (MQ=255) cgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAAtgc < 1:2291744/53‑1 (MQ=255) cgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAggg > 1:621022/1‑70 (MQ=255) cgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAggg > 1:2476061/1‑70 (MQ=255) ttATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAg > 1:3452985/1‑63 (MQ=255) ttATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAg > 1:3046235/1‑63 (MQ=255) ttATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAg > 1:2976103/1‑63 (MQ=255) ttATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAg > 1:2692666/1‑63 (MQ=255) | GAGCTTCTTTAGCGATACGTAGCATGGGCAATATCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGG > minE/1666732‑1666865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |