| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,996 | A→T | 9.7% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,996 | 0 | A | T | 9.7% | 172.3 / 9.7 | 62 | intergenic (‑88/‑18) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (36/20); new base T (4/2); total (40/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217927‑218065 | gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg > 1:1222233/1‑71 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAgg > 1:215928/1‑71 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg < 1:1920259/70‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTAcaca < 1:1886206/69‑1 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCg < 1:155539/70‑1 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt < 1:1578321/71‑1 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTc > 1:2123645/1‑71 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTc < 1:1775655/71‑1 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt < 1:3709668/70‑1 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt < 1:2880065/70‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGAc < 1:1749249/71‑1 (MQ=255) cAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg > 1:1165205/1‑69 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTa < 1:915251/71‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTa < 1:930339/71‑1 (MQ=255) tCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaat > 1:3099758/1‑71 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAgg > 1:2683932/1‑49 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAgg > 1:992667/1‑49 (MQ=255) gCCTCAGCAATCCGAAACCGCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaatg < 1:1040046/70‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaatg > 1:2317458/1‑69 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaatg > 1:835169/1‑68 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGc > 1:676341/1‑46 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:568172/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:2418661/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:1916021/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:964734/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:2312940/1‑71 (MQ=255) caGCGATCCGATATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:1024586/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGTTAATGAATGAAgac < 1:2919111/71‑1 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2118770/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2757676/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:3698327/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2099471/1‑71 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacgac > 1:2616744/1‑71 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:383285/1‑71 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:3480905/1‑71 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGcaca > 1:767015/1‑35 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGcaca > 1:389507/1‑35 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:2786662/64‑1 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTa > 1:485452/1‑49 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa > 1:516419/1‑58 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGTACGTGGTAATGAATGAAGACGACTACaaaa > 1:2242711/1‑70 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa < 1:2723146/70‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa < 1:3385195/70‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1292249/1‑70 (MQ=255) tcACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:3583781/35‑1 (MQ=39) tcACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:1239757/35‑1 (MQ=39) cACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:2429495/1‑68 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaaga < 1:1578328/71‑1 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGCCGACTACAAAATaag > 1:349178/1‑69 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacgac > 1:1752202/1‑57 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:544938/1‑59 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:2053138/1‑59 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAagaaga < 1:3267037/70‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:3335552/1‑71 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:2942886/1‑70 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:1323149/1‑70 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAgg < 1:25306/71‑1 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:2129699/1‑52 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:3220188/1‑45 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2115603/1‑45 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaa > 1:2931290/1‑65 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgca < 1:302336/70‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:1231078/71‑1 (MQ=255) gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:880816/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg > 1:2291139/1‑61 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:533800/1‑70 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:530657/1‑70 (MQ=255) | GCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217927‑218065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |