| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,695,639 | T→C | 46.4% | E124G (GAG→GGG) | yfhQ ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,695,639 | 0 | T | C | 46.4% | 9.2 / 97.3 | 69 | E124G (GAG→GGG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (22/15); new base C (20/12); total (42/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGC > minE/1695569‑1695706 | cATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAct < 1:810517/71‑1 (MQ=255)cATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAct < 1:2042335/71‑1 (MQ=255) ccAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCa < 1:2094863/71‑1 (MQ=255) ccAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCa < 1:743121/71‑1 (MQ=255) ccAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCa < 1:3080958/71‑1 (MQ=255) cAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCa < 1:531429/70‑1 (MQ=255) gTTCAGCGCGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTg > 1:2480208/1‑70 (MQ=255) ttCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGt < 1:227422/71‑1 (MQ=255) cAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTc < 1:2110550/70‑1 (MQ=255) aGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCa < 1:1471628/70‑1 (MQ=255) cGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGc > 1:1045485/1‑71 (MQ=255) gAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGcc < 1:2748520/71‑1 (MQ=255) aGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGcc > 1:3625939/1‑70 (MQ=255) aGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCg > 1:2989475/1‑71 (MQ=255) tCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTc > 1:275283/1‑71 (MQ=255) gggTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgcg < 1:3148538/71‑1 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgcg > 1:3493373/1‑68 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:182416/1‑69 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:851410/1‑69 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGAcc < 1:317713/71‑1 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTc > 1:1647038/1‑63 (MQ=255) gCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAacac > 1:1968475/1‑71 (MQ=255) cAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAacac > 1:2831237/1‑70 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc < 1:3409430/51‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc < 1:2226633/51‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc < 1:3436616/51‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc < 1:2959151/51‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:2140501/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:1258708/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:1024722/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:1971758/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:925438/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:627162/1‑46 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:1427290/1‑46 (MQ=255) cgcgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTcgcg < 1:709696/57‑1 (MQ=255) gcgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgcgc > 1:705913/1‑71 (MQ=255) gcgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgcgc > 1:1886864/1‑71 (MQ=255) gcgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgcgc > 1:3436664/1‑71 (MQ=255) gcgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgcgc > 1:176674/1‑71 (MQ=255) gcgACATGAAAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgcg < 1:26004/70‑1 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGa > 1:399337/1‑40 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc > 1:623328/1‑41 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc > 1:1210703/1‑41 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc > 1:2907832/1‑41 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc > 1:1987026/1‑41 (MQ=255) tGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:905891/1‑51 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:1774251/1‑56 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:1756193/1‑56 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:2240129/1‑56 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:3013912/1‑56 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:2036309/1‑56 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac > 1:3518973/1‑55 (MQ=255) aTAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgc > 1:313746/1‑61 (MQ=255) aTAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACAcc < 1:1540999/58‑1 (MQ=255) tAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc < 1:33131/38‑1 (MQ=255) tAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACAcc < 1:2339373/57‑1 (MQ=255) aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:2746465/71‑1 (MQ=255) aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:1799695/71‑1 (MQ=255) aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:3078485/71‑1 (MQ=255) tGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAtt < 1:362530/71‑1 (MQ=255) cATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:2179767/1‑42 (MQ=255) cATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:542446/1‑42 (MQ=255) cATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:432334/1‑42 (MQ=255) tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTcgcg < 1:1276013/39‑1 (MQ=255) tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:681890/43‑1 (MQ=255) tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc > 1:2414561/1‑69 (MQ=255) tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCg > 1:158328/1‑71 (MQ=255) tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCg < 1:2902171/71‑1 (MQ=255) ttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc > 1:3483928/1‑68 (MQ=255) gCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAacac > 1:3452407/1‑43 (MQ=255) aaCCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTCCCGCTTCAGc < 1:3316197/71‑1 (MQ=255) | CATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGC > minE/1695569‑1695706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |