| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,735,602 | T→C | 43.5% | E157E (GAA→GAG) | era ← | membrane‑associated, 16S rRNA‑binding GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,735,602 | 0 | T | C | 43.5% | 33.3 / 120.2 | 92 | E157E (GAA→GAG) | era | membrane‑associated, 16S rRNA‑binding GTPase |
| Reads supporting (aligned to +/- strand): ref base T (30/22); new base C (24/16); total (54/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
GAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAGA > minE/1735533‑1735671 | gAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt > 1:3522825/1‑71 (MQ=255) aGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCg < 1:1718604/71‑1 (MQ=255) gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCgg < 1:719981/71‑1 (MQ=255) gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCgg < 1:1744832/71‑1 (MQ=255) tgatgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg < 1:3121932/70‑1 (MQ=255) tgatgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg < 1:3523929/70‑1 (MQ=255) atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCgg > 1:1143275/1‑68 (MQ=255) atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCag < 1:2763884/71‑1 (MQ=255) atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCACGGTTTCgg > 1:2157977/1‑68 (MQ=255) tgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCaga < 1:3092043/71‑1 (MQ=255) tgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCa > 1:1506595/1‑69 (MQ=255) gaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCa > 1:2345840/1‑68 (MQ=255) gTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGAt > 1:224141/1‑71 (MQ=255) tCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGtt < 1:1607993/59‑1 (MQ=255) gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTgg > 1:2509340/1‑71 (MQ=255) tCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCAc > 1:1584689/1‑71 (MQ=255) tCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCAc > 1:1413722/1‑71 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAg > 1:1973209/1‑70 (MQ=255) cTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:705798/70‑1 (MQ=255) ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:1865246/1‑69 (MQ=255) tACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAg > 1:2372871/1‑71 (MQ=255) aCGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt > 1:316569/1‑71 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt < 1:3488780/71‑1 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt < 1:3267010/71‑1 (MQ=255) aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt < 1:3146095/71‑1 (MQ=255) aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt < 1:2228383/71‑1 (MQ=255) aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt < 1:3182828/71‑1 (MQ=255) cGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc < 1:3448656/71‑1 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:2462503/1‑71 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGc > 1:1867485/1‑38 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGc > 1:2439129/1‑38 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTg < 1:2477736/71‑1 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTCCATCTg < 1:6518/71‑1 (MQ=255) tGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGc > 1:2066809/1‑71 (MQ=255) tGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGc > 1:1697566/1‑71 (MQ=255) gCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGa < 1:3178433/58‑1 (MQ=255) gCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGa < 1:2662010/58‑1 (MQ=255) gCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGa < 1:3120415/58‑1 (MQ=255) gCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGa < 1:3420126/58‑1 (MQ=255) cGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAg > 1:2447411/1‑58 (MQ=255) cGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAg > 1:1832252/1‑58 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:2147990/1‑71 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:1392277/1‑71 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATc > 1:1313428/1‑49 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATc > 1:1276170/1‑49 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc < 1:10559/71‑1 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGc > 1:3453465/1‑41 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:508850/1‑70 (MQ=255) cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:1332152/1‑70 (MQ=255) aaTAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCa < 1:2025576/71‑1 (MQ=255) aaTAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTg < 1:1738759/38‑1 (MQ=255) aTAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:2331248/1‑61 (MQ=255) aTAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:3296019/1‑61 (MQ=255) aTAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTAGCCAg > 1:3406707/1‑71 (MQ=255) tAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:3339420/56‑1 (MQ=255) gtgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:2840752/1‑56 (MQ=255) gtgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:2692602/1‑56 (MQ=255) gtgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3641699/1‑56 (MQ=255) gtgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGa < 1:3581932/70‑1 (MQ=255) gtgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCAAGCCAgat < 1:3559146/70‑3 (MQ=255) gtgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCAAGCCAgat < 1:2234110/70‑3 (MQ=255) tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCa > 1:653248/1‑66 (MQ=255) tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAAc > 1:2312735/1‑71 (MQ=255) tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:3415562/70‑1 (MQ=255) tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:1545707/70‑1 (MQ=255) tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:1456210/70‑1 (MQ=255) tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:2640139/1‑71 (MQ=255) aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGa < 1:3087261/41‑1 (MQ=255) aCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:2866324/1‑51 (MQ=255) aCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAg > 1:3296497/1‑71 (MQ=255) cATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAgg > 1:920530/1‑71 (MQ=255) cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:1455830/1‑41 (MQ=255) cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:2530983/1‑41 (MQ=255) cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:3550823/1‑41 (MQ=255) cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:169688/1‑41 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:304869/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:3040164/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:1217916/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:657089/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:2331343/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:560385/1‑39 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:1749832/1‑39 (MQ=255) cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:1160126/1‑46 (MQ=255) cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:707689/1‑46 (MQ=255) cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:2785915/1‑46 (MQ=255) cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTgg > 1:3059015/1‑49 (MQ=255) cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGc < 1:1759325/70‑1 (MQ=255) cccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt < 1:608811/38‑1 (MQ=255) cccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt < 1:716163/38‑1 (MQ=255) cccGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgca < 1:2843753/71‑1 (MQ=255) cGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc < 1:3109782/41‑1 (MQ=255) cGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcagc < 1:2678257/71‑1 (MQ=255) ggTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag > 1:441053/1‑69 (MQ=255) gTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGcagcag < 1:3435533/71‑1 (MQ=255) tttCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:1536106/54‑1 (MQ=255) tttCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:2910341/54‑1 (MQ=255) tttCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAGa < 1:2119647/71‑1 (MQ=255) | GAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAGA > minE/1735533‑1735671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |