| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,807,994 | C→T | 9.1% | intergenic (‑96/+54) | luxS ← / ← gshA | S‑ribosylhomocysteinase/gamma‑glutamate‑cysteine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,807,994 | 0 | C | T | 9.1% | 194.7 / 8.7 | 66 | intergenic (‑96/+54) | luxS/gshA | S‑ribosylhomocysteinase/gamma‑glutamate‑cysteine ligase |
| Reads supporting (aligned to +/- strand): ref base C (21/39); new base T (2/4); total (23/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTT > minE/1807924‑1808060 | aaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATcc < 1:405129/71‑1 (MQ=255) aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCt > 1:1148587/1‑70 (MQ=255) aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCt > 1:2863482/1‑70 (MQ=255) aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCt > 1:1705919/1‑70 (MQ=255) aaCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCag < 1:900332/70‑1 (MQ=255) aaCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCag < 1:723498/70‑1 (MQ=255) aaCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCa > 1:2841036/1‑69 (MQ=255) aaCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCa > 1:2828300/1‑69 (MQ=255) aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGaa < 1:2814013/64‑1 (MQ=255) aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGaa < 1:1803226/64‑1 (MQ=255) aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGaa < 1:2537464/64‑1 (MQ=255) aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGaa < 1:2345543/64‑1 (MQ=255) ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAATTCAggga < 1:2570641/71‑1 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAATTCAggga < 1:530720/65‑1 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAATTCAggga < 1:1372473/65‑1 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAATTCAggga < 1:2651309/65‑1 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAt > 1:631393/1‑71 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAt > 1:3590223/1‑71 (MQ=255) cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAt > 1:1156614/1‑71 (MQ=255) cccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAtt < 1:1389405/69‑1 (MQ=255) cAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTgtt < 1:3129601/52‑3 (MQ=255) tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:1933337/71‑1 (MQ=255) tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc > 1:2813021/1‑71 (MQ=255) cTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc > 1:2625708/1‑70 (MQ=255) tGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAAtt > 1:2743370/1‑44 (MQ=255) tGAGTATATGAAAGACGCGCATTTGTTATCATCATCTCTGAAtt > 1:407692/1‑44 (MQ=255) tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc > 1:2392413/1‑69 (MQ=255) tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc > 1:3057796/1‑69 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:3660041/68‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:3271884/68‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:1900570/68‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGa < 1:2918261/69‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAg < 1:3419298/70‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAg < 1:3432084/70‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAg < 1:3456758/70‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt < 1:3484880/71‑1 (MQ=255) aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt < 1:2550997/71‑1 (MQ=255) gTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt < 1:1755226/70‑1 (MQ=255) atGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:3090550/63‑1 (MQ=255) atGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:1618614/63‑1 (MQ=255) tGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg < 1:698450/62‑1 (MQ=255) gAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttt > 1:45190/1‑71 (MQ=255) gAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttt > 1:1897471/1‑71 (MQ=255) aaaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt > 1:2206986/1‑71 (MQ=255) aaaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt > 1:737189/1‑71 (MQ=255) aaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt < 1:2995433/71‑1 (MQ=255) aaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt > 1:434125/1‑70 (MQ=255) gACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttct < 1:1572661/71‑1 (MQ=255) gcgcATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACg > 1:3728925/1‑54 (MQ=255) cgcATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAg < 1:1024299/55‑1 (MQ=255) gcATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAg < 1:2916145/54‑1 (MQ=255) tttGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctttt < 1:1932340/66‑1 (MQ=255) tttGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctttt < 1:2242654/66‑1 (MQ=255) tttGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctttt < 1:2459238/66‑1 (MQ=255) tttGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctttt < 1:2602025/66‑1 (MQ=255) gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:2603497/45‑1 (MQ=255) gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:1627021/45‑1 (MQ=255) gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:2330466/45‑1 (MQ=255) gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:3452931/45‑1 (MQ=255) ttATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:2607980/44‑1 (MQ=255) ttATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc < 1:3681731/44‑1 (MQ=255) tATCATCATCCCTGAATTCAGAGATGAAATTTTGGc > 1:822831/1‑36 (MQ=255) tATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGa > 1:3122521/1‑45 (MQ=255) tATCATCATCCCTGAATTCAGAGATGAAA‑TTTGGCCACTCACGa > 1:1877195/1‑44 (MQ=255) tcatcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg < 1:3521033/71‑1 (MQ=255) atcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtgt < 1:2665247/70‑1 (MQ=255) atcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtttt < 1:3487767/70‑1 (MQ=255) | AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTT > minE/1807924‑1808060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |