| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,852,438 | C→T | 11.2% | A395T (GCG→ACG) | ygcL ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,852,438 | 0 | C | T | 11.2% | 225.8 / 16.7 | 80 | A395T (GCG→ACG) | ygcL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/41); new base T (7/2); total (37/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
ATGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTG > minE/1852373‑1852507 | aTGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGc > 1:1362241/1‑71 (MQ=255)aTGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCtt < 1:2380552/69‑1 (MQ=255)aTGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCtt < 1:2955707/69‑1 (MQ=255) tGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCg > 1:1553786/1‑71 (MQ=255) atgagaCTGCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTaa < 1:2121405/68‑1 (MQ=255) agagACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAgg > 1:3109816/1‑71 (MQ=255) cGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTta > 1:944444/1‑71 (MQ=255) cGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTta > 1:3465085/1‑71 (MQ=255) ggCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTtat < 1:3703216/71‑1 (MQ=255) tGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCa < 1:22768/69‑1 (MQ=255) aaGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAAcc < 1:2141484/71‑1 (MQ=255) aGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGTTTTATATCCCAAAcc > 1:129930/1‑70 (MQ=255) gTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATccc > 1:2503752/1‑64 (MQ=255) tttATTTTTAAACCCTTCTGCAAAGGTATATAACGCCt < 1:1469858/38‑1 (MQ=255) tttATTTTTAAACCCTTCTGCAAAGGTATATAACGCCt < 1:2363283/38‑1 (MQ=255) ttATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGttt < 1:3357218/52‑1 (MQ=255) ttATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGt < 1:1666180/50‑1 (MQ=255) ttATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt > 1:3106002/1‑71 (MQ=255) tATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt > 1:3202885/1‑70 (MQ=255) tATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt > 1:3198532/1‑70 (MQ=255) tATTTTTAAACCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt > 1:2876493/1‑70 (MQ=255) tATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGt < 1:1544725/41‑1 (MQ=255) ttttAAACCCTTCTGCAAAGGTATATAACGCTTTGCGTa < 1:126806/39‑1 (MQ=255) ttttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTa < 1:1512655/39‑1 (MQ=255) ttttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTa < 1:2668566/39‑1 (MQ=255) ttttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCAc > 1:1206660/1‑70 (MQ=255) ttAAACCCTTCTGCAAAGGTATATAACGCCTTGCg > 1:495315/1‑35 (MQ=255) ttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGt > 1:2063841/1‑36 (MQ=255) ttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAg > 1:262998/1‑39 (MQ=255) ttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGt < 1:2483062/44‑1 (MQ=255) ttAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTAt > 1:2132274/1‑71 (MQ=255) tAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGtt > 1:1963821/1‑44 (MQ=255) tAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGtt > 1:812763/1‑44 (MQ=255) aaCCCTTCTGCAAAGGTATATAACGTCTTGCGTAAGGCTGttt < 1:1381108/43‑1 (MQ=255) aaCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTAttt < 1:2315403/70‑1 (MQ=255) aaCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTAttt < 1:630576/70‑1 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTtata < 1:3719977/46‑1 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCAc > 1:3502953/1‑64 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCAc > 1:25436/1‑64 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCAc > 1:522037/1‑64 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCg < 1:1521520/71‑1 (MQ=255) aCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCg < 1:2932711/71‑1 (MQ=255) ccTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACa < 1:3600599/57‑1 (MQ=255) ccTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACa < 1:1651112/57‑1 (MQ=255) ccTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACa < 1:503362/57‑1 (MQ=255) ttCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTa > 1:594727/1‑71 (MQ=255) tCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTAt > 1:3611593/1‑71 (MQ=255) tCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTAt > 1:2303735/1‑71 (MQ=255) cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAAc > 1:903790/1‑48 (MQ=255) cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAAc > 1:112288/1‑48 (MQ=255) cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTa < 1:1729611/69‑1 (MQ=255) cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTa < 1:174648/69‑1 (MQ=255) cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTAt > 1:732453/1‑70 (MQ=255) gCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt < 1:2821360/53‑1 (MQ=255) gCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGt < 1:3310911/53‑1 (MQ=255) cAAAGGTATATAACGCCTTGCGTAAGGCTGTTTta > 1:2684863/1‑35 (MQ=255) cAAAGGTATATAACGCCTTGCGTAAGGCTGTTTta > 1:2042082/1‑35 (MQ=255) cAAAGGTATATAACGCCTTGCGTAAGGCTGTTTta > 1:3004223/1‑35 (MQ=255) aaGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATc > 1:324889/1‑66 (MQ=255) aaGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACAtt > 1:2656474/1‑71 (MQ=255) aaGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACAtt > 1:59405/1‑71 (MQ=255) ggTATATAACGCCTTGCGTAAGGCTGTTTTATATcc < 1:3689407/36‑1 (MQ=255) tatataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCg < 1:1865561/71‑1 (MQ=255) tatataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCg < 1:2245038/71‑1 (MQ=255) tataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCaa < 1:3147199/40‑1 (MQ=255) tataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCaa < 1:924606/40‑1 (MQ=255) tataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGt < 1:1185052/70‑1 (MQ=255) ataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTAt < 1:726151/58‑1 (MQ=255) ataACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTAt < 1:2052848/58‑1 (MQ=255) taACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTa < 1:1860424/69‑1 (MQ=255) taACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTAtt > 1:1634236/1‑71 (MQ=255) taACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTAtt < 1:2205134/71‑1 (MQ=255) aaCGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGt < 1:1866761/52‑1 (MQ=255) aaCGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGt < 1:2406548/52‑1 (MQ=255) aaCGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTa < 1:3085277/55‑1 (MQ=255) aCGTCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTg > 1:3582379/1‑62 (MQ=255) aCGTCTAGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTAttgt > 1:2481270/1‑71 (MQ=255) aCGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTa < 1:2218562/54‑1 (MQ=255) aCGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTAttg < 1:2939102/70‑1 (MQ=255) gCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTAttgttg > 1:2814364/1‑71 (MQ=255) | ATGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTG > minE/1852373‑1852507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |