| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,869,845 | G→A | 14.9% | intergenic (‑90/‑229) | ygcW ← / → yqcE | predicted deoxygluconate dehydrogenase/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,869,845 | 0 | G | A | 14.9% | 124.9 / 12.5 | 47 | intergenic (‑90/‑229) | ygcW/yqcE | predicted deoxygluconate dehydrogenase/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (15/25); new base A (3/4); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01 | |||||||||||
AAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTG > minE/1869775‑1869912 | aaGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTg > 1:1814645/1‑71 (MQ=255) cAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTg > 1:2239880/1‑67 (MQ=255) aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATa < 1:1804641/71‑1 (MQ=255) aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtt < 1:2455263/71‑1 (MQ=255) aaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATTATCTCTGGTAACTGGAGATAAttt < 1:3046743/71‑1 (MQ=255) agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt > 1:2418009/1‑66 (MQ=255) agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt > 1:1892464/1‑66 (MQ=255) tAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt < 1:63067/62‑1 (MQ=18) aaCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg > 1:2417531/1‑71 (MQ=255) cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTg < 1:2274610/48‑1 (MQ=255) cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc < 1:971573/71‑1 (MQ=255) cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc < 1:3019708/71‑1 (MQ=255) ccGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa < 1:3161944/48‑1 (MQ=18) ccGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa < 1:358919/48‑1 (MQ=18) ccGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa < 1:540346/48‑1 (MQ=18) cgcgAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa < 1:2109267/47‑1 (MQ=17) gagGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt < 1:2046539/53‑1 (MQ=255) gagGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt < 1:2564393/53‑1 (MQ=255) agGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATaa < 1:302233/54‑1 (MQ=255) agGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATaa < 1:3494779/54‑1 (MQ=255) gggAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg < 1:451818/70‑1 (MQ=255) aaGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTaa > 1:2668396/1‑65 (MQ=255) aGTTACCTTTCTCATTATCTCTGGTAACTGGAGATaa > 1:1540389/1‑37 (MQ=255) ttACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt < 1:1835682/38‑1 (MQ=255) tACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt < 1:3293523/52‑1 (MQ=255) tttCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg < 1:425748/53‑1 (MQ=255) tctcATTATCTCTGGTAACTGGAGATAATTTTTAg > 1:1581150/1‑35 (MQ=255) aTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg < 1:6439/70‑1 (MQ=255) aTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt < 1:760187/69‑1 (MQ=255) aTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt < 1:2383951/69‑1 (MQ=255) aTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAg < 1:987674/68‑1 (MQ=255) ttATCTCTGGTAACTGGAGATAATTTTTAGCATaa < 1:309263/35‑1 (MQ=255) ttATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCa < 1:2783323/40‑1 (MQ=255) ttATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCa < 1:1353227/40‑1 (MQ=255) ttATCTCCAGTTACCAGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt > 1:81907/1‑70 (MQ=37) ttATCTCCAGTTACCAGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg > 1:3003727/1‑69 (MQ=25) ttATCTCCAGTTACCAGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg > 1:2140658/1‑69 (MQ=25) aTCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATa > 1:3629166/1‑40 (MQ=255) aTCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATa > 1:1828557/1‑40 (MQ=255) aTCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa < 1:3043639/70‑1 (MQ=255) tctcTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata > 1:394953/1‑71 (MQ=255) tctGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATAtt > 1:1316923/1‑71 (MQ=255) tctGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATAtt > 1:827119/1‑71 (MQ=255) ctGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAt > 1:1221890/1‑45 (MQ=255) ctGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAt > 1:2458626/1‑45 (MQ=255) tGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGatat < 1:3005014/68‑1 (MQ=255) gTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa < 1:2113332/71‑1 (MQ=255) aaCTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt > 1:821376/1‑71 (MQ=255) aaCTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt > 1:1322846/1‑71 (MQ=255) aCTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTg < 1:1766303/71‑1 (MQ=255) | AAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTG > minE/1869775‑1869912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |