| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,930,802 | G→A | 9.1% | T120T (ACC→ACT) | kduI ← | predicted 5‑keto 4‑deoxyuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,930,802 | 0 | G | A | 9.1% | 200.4 / 8.7 | 66 | T120T (ACC→ACT) | kduI | predicted 5‑keto 4‑deoxyuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (27/33); new base A (4/2); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATT > minE/1930733‑1930871 | tgtgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGGCGGAGTGCCgg < 1:2095783/71‑1 (MQ=255)tgtgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCgg < 1:907491/71‑1 (MQ=255)tgtgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCgg > 1:2825677/1‑71 (MQ=255)tgtgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCgg > 1:1168393/1‑71 (MQ=255) gtgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCgg > 1:2970096/1‑70 (MQ=255) tgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCgg < 1:2387075/69‑1 (MQ=255) tgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTa < 1:3065485/71‑1 (MQ=255) tgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTa < 1:3634700/71‑1 (MQ=255) aCTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTa > 1:686461/1‑69 (MQ=255) aCTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTTTc < 1:2685976/71‑1 (MQ=255) cTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCa < 1:3178908/71‑1 (MQ=255) cTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCa < 1:403974/71‑1 (MQ=255) ttttttGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCaa > 1:513165/1‑71 (MQ=255) ttttttGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCaa > 1:437467/1‑71 (MQ=255) ttttGGTGGGATACGTCGTATGCTCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCaa < 1:2277962/69‑1 (MQ=255) ttttGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCaa < 1:3387307/69‑1 (MQ=255) ttttGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCaa < 1:142619/69‑1 (MQ=255) ggtggGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTg < 1:81791/70‑1 (MQ=255) ggtggGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTg < 1:3431682/70‑1 (MQ=255) ggATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCa > 1:3319498/1‑69 (MQ=255) ggATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCa > 1:3469807/1‑69 (MQ=255) aTACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGGCGGTATCAATACTGGCAAAGa > 1:1744217/1‑71 (MQ=255) tACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGAc < 1:3226122/71‑1 (MQ=255) aCGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCAGTATTGATAc > 1:449108/1‑60 (MQ=255) aCGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCAGTATTGATAc > 1:1164403/1‑60 (MQ=255) cgtcgtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCaaa > 1:3605944/1‑66 (MQ=255) cgtcgtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACa < 1:2988316/70‑1 (MQ=255) cgtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActt > 1:1895446/1‑71 (MQ=255) gtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCa < 1:2423012/60‑1 (MQ=255) gtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActtc > 1:612928/1‑71 (MQ=255) tATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActtct < 1:2671428/71‑1 (MQ=255) tATGCGCGGGAGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActtct < 1:947642/71‑1 (MQ=255) tGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActtctt < 1:565002/70‑1 (MQ=255) gcgcGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGAc < 1:1352139/61‑1 (MQ=255) gcgGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGc < 1:2424189/71‑1 (MQ=255) cgGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAAt < 1:3001819/46‑1 (MQ=255) gTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCt < 1:2701017/71‑1 (MQ=255) tGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCtt < 1:2224317/71‑1 (MQ=255) cgcAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCtttt > 1:885397/1‑62 (MQ=255) gcAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTAc > 1:137173/1‑71 (MQ=255) gcAATTGTAATAAAACTTCGCCGGAGTGCCAGTATTGATAcc < 1:2577009/42‑2 (MQ=25) gcAATTGTAATAAAACTTCGCCGGAGTGCCAGTATTGATAcc < 1:2250560/42‑2 (MQ=25) aaTTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCttt > 1:3481515/1‑67 (MQ=255) aaTTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTAcc > 1:3707324/1‑70 (MQ=255) ttGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCaa < 1:2236018/70‑1 (MQ=255) ttGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCaa > 1:2268791/1‑70 (MQ=255) ttGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCaa > 1:2807280/1‑70 (MQ=255) ataaAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATa > 1:2245411/1‑69 (MQ=255) ataaAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATa > 1:3545943/1‑69 (MQ=255) ataaAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACa < 1:2289020/71‑1 (MQ=255) ataaAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACa < 1:3182695/71‑1 (MQ=255) aaCTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCttt > 1:1703522/1‑47 (MQ=255) cTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGt > 1:1703172/1‑71 (MQ=255) tCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTcg > 1:3147437/1‑71 (MQ=255) ccGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCttt > 1:2257988/1‑49 (MQ=255) ccGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCgg > 1:608095/1‑71 (MQ=255) cGGAGTGCCGGTATCAATACTGGCGAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt > 1:3392912/1‑71 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCttt > 1:3645956/1‑48 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCttt > 1:88924/1‑48 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCttt > 1:776939/1‑48 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCTACATACAGGGCGTCGCGGt < 1:2523111/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:458369/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:237062/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:2336836/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:2407159/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:3167692/71‑1 (MQ=255) cGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGt < 1:3684194/71‑1 (MQ=255) gAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTAcc < 1:3445148/49‑1 (MQ=255) gAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGa > 1:3327075/1‑71 (MQ=255) tGCCAGTATTGATACCGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGAcc > 1:3200235/1‑70 (MQ=38) tGCCAGTATTGATACCGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGAcc > 1:1006146/1‑70 (MQ=38) cGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGAtt < 1:1066341/71‑1 (MQ=255) | TGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATT > minE/1930733‑1930871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |