| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,042,434 | G→A | 7.3% | intergenic (+25/+18) | yqiI → / ← glgS | conserved hypothetical protein/predicted glycogen synthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,042,434 | 0 | G | A | 7.3% | 168.0 / 2.1 | 54 | intergenic (+25/+18) | yqiI/glgS | conserved hypothetical protein/predicted glycogen synthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (21/29); new base A (2/2); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAG > minE/2042365‑2042503 | ggACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGc < 1:1978340/71‑1 (MQ=255)ggACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGc < 1:805324/71‑1 (MQ=255)ggACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGc < 1:3187351/71‑1 (MQ=255) gACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGc > 1:1945862/1‑70 (MQ=255) aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGcc < 1:1469672/70‑1 (MQ=255) aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGcc < 1:190154/70‑1 (MQ=255) ccGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCAt < 1:3511188/71‑1 (MQ=255) cGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATc < 1:2921990/71‑1 (MQ=255) ttAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAgg > 1:1070606/1‑71 (MQ=255) ttAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAgg > 1:701341/1‑71 (MQ=255) tAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCg < 1:3273886/71‑1 (MQ=255) tAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCg < 1:2901664/71‑1 (MQ=255) aaGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAg > 1:2359344/1‑64 (MQ=255) aaGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAg > 1:352614/1‑64 (MQ=255) aaGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGt > 1:1361337/1‑70 (MQ=255) gACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTtat < 1:1190105/71‑1 (MQ=255) aCGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTtata > 1:844686/1‑71 (MQ=255) cTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGc < 1:1295658/47‑1 (MQ=255) cTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGc < 1:520781/70‑1 (MQ=255) tGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGctc > 1:3181041/1‑71 (MQ=255) tCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc < 1:747529/71‑1 (MQ=255) aCCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGt < 1:2732033/50‑1 (MQ=255) aCCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTcc < 1:3476240/70‑1 (MQ=255) aCCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc > 1:3643146/1‑69 (MQ=255) ataACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAg < 1:1649378/68‑1 (MQ=255) ataACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAg < 1:1428593/68‑1 (MQ=255) ataACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAg < 1:3389900/68‑1 (MQ=255) ataACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctc > 1:1642660/1‑71 (MQ=255) ataACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctc > 1:1579670/1‑71 (MQ=255) aCTAGTATCTAATACAAACACTAAAACGGGCCATCAGGc > 1:2520414/1‑39 (MQ=255) aCTAGTATCTAATACAAACACTAAAACGGGCCATCAGGc > 1:2586466/1‑39 (MQ=255) aCTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctctct < 1:2650749/71‑1 (MQ=255) tAGTATCTAATACAAACACTAAAACGGGCCATCAGGc > 1:681119/1‑37 (MQ=255) tAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAAc < 1:2311405/51‑1 (MQ=255) tAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAAc < 1:2118050/51‑1 (MQ=255) aTACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatca < 1:683524/71‑1 (MQ=255) aTACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGc < 1:2408770/67‑1 (MQ=255) tACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatc < 1:1293595/69‑1 (MQ=255) tACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatc < 1:3333605/69‑1 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCa > 1:602686/1‑36 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt < 1:2035602/71‑1 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt < 1:2933160/71‑1 (MQ=255) cAAACACTAAAACGGACCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt > 1:3157438/1‑71 (MQ=255) cAAACACTAAAACGGACCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt > 1:3608714/1‑71 (MQ=255) aaaCACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTg > 1:2294168/1‑71 (MQ=255) aaCACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCGTCATCTGt < 1:1829182/71‑1 (MQ=255) acTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGa > 1:823842/1‑71 (MQ=255) tAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc > 1:3121205/1‑70 (MQ=255) tAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc > 1:2653032/1‑70 (MQ=255) aaaCGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCt > 1:3278011/1‑53 (MQ=255) aaaCGGACCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACaa < 1:2958320/70‑1 (MQ=255) aaaCGGACCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACaa < 1:2459091/70‑1 (MQ=255) aCGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc > 1:1475204/1‑71 (MQ=255) cGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAcc < 1:1743299/71‑1 (MQ=255) ggCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAg < 1:2524075/71‑1 (MQ=255) gCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTg < 1:2518098/57‑1 (MQ=255) gCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTg < 1:3149551/57‑1 (MQ=255) | GGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAG > minE/2042365‑2042503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |