| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,042,842 | T→A | 13.3% | intergenic (‑190/‑79) | glgS ← / → yqiJ | predicted glycogen synthesis protein/predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,042,842 | 0 | T | A | 13.3% | 119.8 / 10.5 | 45 | intergenic (‑190/‑79) | glgS/yqiJ | predicted glycogen synthesis protein/predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (19/20); new base A (3/3); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
ACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAT > minE/2042773‑2042909 | acTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatt < 1:2137477/71‑1 (MQ=255) tttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttaataa > 1:1339878/1‑70 (MQ=255) tttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttaataa > 1:2195738/1‑70 (MQ=255) tttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt < 1:3680133/70‑1 (MQ=255) ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:378569/1‑71 (MQ=255) ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:2219502/1‑71 (MQ=255) ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:159412/1‑71 (MQ=255) gTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:2905675/1‑68 (MQ=255) aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca > 1:2128573/1‑67 (MQ=255) aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca > 1:3594471/1‑67 (MQ=255) aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATa < 1:3417513/71‑1 (MQ=255) tAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATaaa > 1:374216/1‑70 (MQ=255) aaaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg < 1:1044298/70‑1 (MQ=255) aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTa < 1:807150/71‑1 (MQ=255) aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa < 1:3104638/71‑1 (MQ=255) aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa < 1:1768872/71‑1 (MQ=255) ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa < 1:877496/71‑1 (MQ=255) ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa < 1:201624/71‑1 (MQ=255) cATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt < 1:1640929/59‑1 (MQ=255) tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatta > 1:516764/1‑46 (MQ=255) tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg < 1:3465889/71‑1 (MQ=255) atTAAAAGATTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg > 1:1482031/1‑61 (MQ=255) atTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg > 1:1117647/1‑70 (MQ=255) ttAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGa < 1:3370330/70‑1 (MQ=255) tAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGaaa > 1:1551535/1‑71 (MQ=255) aCTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa < 1:2843360/71‑1 (MQ=255) tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACa > 1:3294860/1‑69 (MQ=255) tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:2690107/1‑35 (MQ=255) tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa > 1:1843199/1‑35 (MQ=255) ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt < 1:1643102/40‑1 (MQ=255) ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt < 1:1413788/69‑1 (MQ=255) ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt < 1:1618637/69‑1 (MQ=255) ccAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg > 1:540943/1‑47 (MQ=255) aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat < 1:280158/71‑1 (MQ=255) aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat < 1:429296/71‑1 (MQ=255) aCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc < 1:3688374/71‑1 (MQ=255) tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc < 1:1184487/66‑1 (MQ=255) tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt > 1:2797279/1‑66 (MQ=255) tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt > 1:3545435/1‑70 (MQ=255) tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt > 1:662451/1‑70 (MQ=255) aaCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTa > 1:1093924/1‑70 (MQ=255) aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaaa > 1:829079/1‑71 (MQ=255) aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa < 1:2417443/70‑1 (MQ=255) aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa < 1:2529947/70‑1 (MQ=255) gtgtTAATAATAACACAGGTAAATGTAAGCAAGGa < 1:749202/35‑1 (MQ=17) gtgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa < 1:2270071/52‑1 (MQ=37) tgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa < 1:2635767/51‑1 (MQ=37) gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa > 1:3644511/1‑35 (MQ=18) gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa > 1:1419580/1‑35 (MQ=18) gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa > 1:661771/1‑35 (MQ=18) ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt < 1:2041158/71‑1 (MQ=255) ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt > 1:3708411/1‑71 (MQ=255) ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt < 1:1392834/71‑1 (MQ=255) | ACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAT > minE/2042773‑2042909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |