BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,081,573	C→T	13.3%	A423V (GCA→GTA)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,081,573	0	C	T	13.3%	267.1 / 27.2	98	A423V (GCA→GTA)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base C (42/43); new base T (6/7); total (48/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 104 total aligned reads displayed.

AACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTG  >  minE/2081505‑2081643

aaCGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCAc                                                                      >  1:729440/1‑71 (MQ=255)

aaCGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCAc                                                                      >  1:3060575/1‑71 (MQ=255)

 aCGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACt                                                                     <  1:922075/71‑1 (MQ=255)

 aCGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACt                                                                     >  1:2495337/1‑71 (MQ=255)

 aCGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACt                                                                     >  1:3164233/1‑71 (MQ=255)

  cGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTg                                                                    >  1:87970/1‑71 (MQ=255)

   ggCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   <  1:2507750/71‑1 (MQ=255)

   ggCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   >  1:2766446/1‑71 (MQ=255)

   ggCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   >  1:3043274/1‑71 (MQ=255)

     cAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCTGTGCACTGGCt                                                                 <  1:856960/71‑1 (MQ=255)

     cAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCt                                                                 >  1:592089/1‑71 (MQ=255)

      aaaCGGTTTAACCTGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCt                                                                 <  1:3711619/70‑1 (MQ=255)

        aCGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGac                                                              <  1:1261026/71‑1 (MQ=255)

        aCGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGa                                                               >  1:2919799/1‑70 (MQ=255)

         cGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGaca                                                             >  1:264476/1‑71 (MQ=255)

         cGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGaca                                                             >  1:3608615/1‑71 (MQ=255)

          ggTTTAACCGGGATGTCCGCATGGCTGTCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGacac                                                            >  1:408547/1‑71 (MQ=255)

            tttAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCa                                                          >  1:3176420/1‑71 (MQ=255)

              tAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCAt                                                         <  1:1065380/70‑1 (MQ=255)

              tAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                         <  1:3557015/70‑1 (MQ=255)

              tAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                         >  1:3505326/1‑70 (MQ=255)

              tAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                         <  1:1910243/70‑1 (MQ=255)

              tAACCGGGATGTCCGAATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATc                                                        >  1:188423/1‑71 (MQ=255)

                aCCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCg                                                       <  1:2407693/70‑1 (MQ=255)

                aCCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCg                                                       <  1:2032421/70‑1 (MQ=255)

                aCCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCg                                                       <  1:2954281/70‑1 (MQ=255)

                 ccGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGc                                                     <  1:560366/71‑1 (MQ=255)

                 ccGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGc                                                     <  1:2600962/71‑1 (MQ=255)

                 ccGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGc                                                     >  1:1660252/1‑71 (MQ=255)

                 ccGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGc                                                     >  1:1516090/1‑71 (MQ=255)

                  cGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCt                                                    <  1:769899/71‑1 (MQ=255)

                  cGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCt                                                    <  1:1733391/71‑1 (MQ=255)

                   gtgATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   <  1:2829907/53‑1 (MQ=255)

                   gggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   <  1:3643888/55‑1 (MQ=255)

                    ggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCAc                                                                      >  1:2672281/1‑51 (MQ=255)

                    ggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCAc                                                                      >  1:1206103/1‑51 (MQ=255)

                    ggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTc                                                  <  1:444375/71‑1 (MQ=255)

                    ggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTc                                                  <  1:2104993/71‑1 (MQ=255)

                    ggATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGATGGTTGTCGGTGCAc                                                                      >  1:826676/1‑51 (MQ=255)

                     gATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCtt                                                   >  1:2026594/1‑69 (MQ=255)

                       tGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGc                                               >  1:538117/1‑71 (MQ=255)

                       tGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGc                                               >  1:946790/1‑71 (MQ=255)

                       tGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGc                                               >  1:1532837/1‑71 (MQ=255)

                         tCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGccc                                             <  1:2842253/71‑1 (MQ=255)

                         tCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGcc                                              >  1:1095191/1‑70 (MQ=255)

                           cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCg                                            <  1:1683956/70‑1 (MQ=255)

                           cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCg                                            <  1:179191/70‑1 (MQ=255)

                           cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                            <  1:934260/70‑1 (MQ=255)

                           cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGc                                           >  1:1536797/1‑71 (MQ=255)

                           cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGc                                           >  1:1295870/1‑71 (MQ=255)

                             cATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                         >  1:1852227/1‑55 (MQ=255)

                             cATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                         >  1:1760772/1‑55 (MQ=255)

                              aTGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCGCTGt                                        >  1:2734881/1‑71 (MQ=255)

                              aTGGCTGGCAAGTACGCTGGTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCGCTGt                                        >  1:1754232/1‑71 (MQ=255)

                               tggctggcAAGTACGCTGTTTGCTCTGGTTGTCGGTGCACTGGCTGACACCATCgg                                                      <  1:1587663/56‑1 (MQ=255)

                                  ctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTgg                                                                   <  1:1233878/40‑1 (MQ=255)

                                  ctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGa                                                               >  1:3029329/1‑44 (MQ=255)

                                  ctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGATTCAGCCCGCTGTTCg                                     >  1:2868912/1‑70 (MQ=255)

                                   tggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTc                                      >  1:1943960/1‑68 (MQ=255)

                                     gcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAg                                  <  1:1051399/70‑1 (MQ=255)

                                     gaaaGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAg                                  <  1:1991360/68‑1 (MQ=255)

                                          tACGCTGTTTGCGCTGGTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                            <  1:1119912/71‑1 (MQ=255)

                                          tACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCg                                     <  1:2531030/62‑1 (MQ=255)

                                          tACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                            <  1:1133229/71‑1 (MQ=255)

                                           aCGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                            >  1:2232419/1‑70 (MQ=255)

                                            cGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGc                           <  1:1401678/70‑1 (MQ=255)

                                             gCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                            <  1:2974746/68‑1 (MQ=255)

                                             gCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAg                         <  1:2541060/71‑1 (MQ=255)

                                               tGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCa                          >  1:3401430/1‑68 (MQ=255)

                                               tGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCa                          >  1:3091332/1‑68 (MQ=255)

                                                gTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtgt                      >  1:3119225/1‑71 (MQ=255)

                                                gTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtgt                      >  1:1710044/1‑71 (MQ=255)

                                                 tttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtgt                      <  1:2606290/70‑1 (MQ=255)

                                                 tttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtgt                      <  1:523389/70‑1 (MQ=255)

                                                   tGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTc                    >  1:1557772/1‑70 (MQ=255)

                                                       cTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCt               <  1:350518/71‑1 (MQ=255)

                                                        tGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTg              >  1:2095231/1‑71 (MQ=255)

                                                        tGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTg              >  1:405549/1‑71 (MQ=255)

                                                         ggTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCGCTGTTc                                      >  1:196839/1‑46 (MQ=255)

                                                          gTTGTCGGTGTACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGtt            <  1:2125716/71‑1 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTc                                      <  1:3204578/45‑1 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTc                                      <  1:1088113/45‑1 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGtt            <  1:3154091/71‑1 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGt             >  1:4079/1‑70 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGt             >  1:3064871/1‑70 (MQ=255)

                                                          gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGt             <  1:2509890/70‑1 (MQ=255)

                                                              tCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGt        <  1:38382/71‑1 (MQ=255)

                                                              tCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGt        <  1:3547863/71‑1 (MQ=255)

                                                               cGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGt        <  1:1820257/70‑1 (MQ=255)

                                                                ggTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTa           >  1:3381111/1‑66 (MQ=255)

                                                                  tGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcgc    <  1:1540746/71‑1 (MQ=255)

                                                                  tGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcgc    >  1:327936/1‑71 (MQ=255)

                                                                  tGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcgc    <  1:3573790/71‑1 (MQ=255)

                                                                  tGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcgc    <  1:3350588/71‑1 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:518205/1‑71 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:1228519/1‑71 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:795718/1‑71 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:1713322/1‑71 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:3469316/1‑71 (MQ=255)

                                                                    cACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg  >  1:2385029/1‑71 (MQ=255)

AACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTG  >  minE/2081505‑2081643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`