| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,088,442 | A→T | 7.0% | G110G (GGA→GGT) | yhaI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,088,442 | 0 | A | T | 7.0% | 202.9 / 6.4 | 71 | G110G (GGA→GGT) | yhaI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (33/33); new base T (3/2); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAA > minE/2088373‑2088510 | gTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGa > 1:1751347/1‑70 (MQ=255) tttATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGaaa < 1:2800223/71‑1 (MQ=255) tATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAAc > 1:656025/1‑70 (MQ=255) aTTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACg < 1:1183070/70‑1 (MQ=255) aTTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACg < 1:908424/70‑1 (MQ=255) ttGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGa < 1:1269827/70‑1 (MQ=255) tggctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCg > 1:1513797/1‑69 (MQ=255) gctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAg < 1:2444319/70‑1 (MQ=255) gctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGt > 1:940797/1‑71 (MQ=255) gTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGttt < 1:3322472/69‑1 (MQ=255) gTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTgg > 1:2094172/1‑71 (MQ=255) gTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTgg > 1:2032576/1‑71 (MQ=255) tCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCa > 1:948818/1‑70 (MQ=255) cTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCaaa > 1:998736/1‑71 (MQ=255) ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGa < 1:877461/51‑1 (MQ=255) ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGa < 1:747196/51‑1 (MQ=255) ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGa < 1:2831140/51‑1 (MQ=255) ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAAt > 1:133507/1‑70 (MQ=255) gTTTTTTTCTGCACAGAAGGTACTTCTTGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAAtt < 1:991033/70‑1 (MQ=255) gTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTa < 1:3614027/71‑1 (MQ=255) gTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTa < 1:493242/71‑1 (MQ=255) tttttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTaa < 1:1883243/71‑1 (MQ=255) tttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAAt < 1:1661962/70‑1 (MQ=255) tttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATc > 1:3649611/1‑71 (MQ=255) cTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAg > 1:1537249/1‑70 (MQ=255) cacaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAt < 1:2860554/70‑1 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGaaa > 1:1454653/1‑35 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGaaa > 1:2225127/1‑35 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGaaa > 1:2387553/1‑35 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTa < 1:2417027/65‑1 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTa < 1:2694569/65‑1 (MQ=255) acaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAt < 1:1860574/69‑1 (MQ=255) aGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTa < 1:3518529/63‑1 (MQ=255) aGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAt > 1:181967/1‑67 (MQ=255) aGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTg > 1:285557/1‑69 (MQ=255) aGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTAGGTTCAAATTAATCTTAGAATTg > 1:382726/1‑69 (MQ=255) gAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTggg > 1:943477/1‑70 (MQ=255) aaGGTACTTCTGGCAGCAATCGTTTCCGTAACGAt > 1:3542184/1‑35 (MQ=21) aaGGTACTTCTGGCAGCAATCGTTTCCGTAACGAt > 1:3636099/1‑35 (MQ=21) aaGGTACTTCTGGCAGCAATCGTTTCCGTAACGAt > 1:554743/1‑35 (MQ=21) aaGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGc > 1:3141962/1‑71 (MQ=255) gTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGc < 1:1259329/68‑1 (MQ=255) cTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCg < 1:682525/66‑1 (MQ=255) cTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCg < 1:3157854/66‑1 (MQ=255) cTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATAtt < 1:1368469/71‑1 (MQ=255) cTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATAtt < 1:2993969/71‑1 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTg > 1:2793462/1‑36 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:3538681/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:2721553/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:1624977/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:2808577/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:2703910/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat > 1:2306034/1‑65 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTc < 1:1741141/70‑1 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCg > 1:2929257/1‑71 (MQ=255) cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCg > 1:695694/1‑71 (MQ=255) agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCt < 1:2947200/71‑1 (MQ=255) agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCt < 1:1493511/71‑1 (MQ=255) cggaTCGTTTCGGTAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCt < 1:2905270/67‑1 (MQ=255) gttCGTTTCCGTAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCt < 1:1861340/66‑1 (MQ=255) aaTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt < 1:2320696/71‑1 (MQ=255) aaTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt > 1:207655/1‑71 (MQ=255) cGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAg < 1:2015858/42‑1 (MQ=255) cGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAg < 1:2011637/42‑1 (MQ=255) gTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttatt < 1:2513754/71‑1 (MQ=255) ttACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAtt < 1:2604305/44‑1 (MQ=255) ttACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttatt > 1:1332242/1‑70 (MQ=255) aCGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTaa > 1:710161/1‑70 (MQ=255) cGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt > 1:2220667/1‑63 (MQ=255) cGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt > 1:2099133/1‑63 (MQ=255) ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGa > 1:3000988/1‑37 (MQ=255) ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGa > 1:1217034/1‑37 (MQ=255) ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGa > 1:905792/1‑37 (MQ=255) ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGa > 1:595692/1‑37 (MQ=255) ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaa < 1:2637351/71‑1 (MQ=255) gAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtt < 1:1601267/59‑1 (MQ=255) | GTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAA > minE/2088373‑2088510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |