| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,136,857 | A→T | 5.1% | intergenic (‑80/+69) | rpsO ← / ← truB | 30S ribosomal subunit protein S15/tRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,136,857 | 0 | A | T | 5.1% | 288.0 / 5.7 | 98 | intergenic (‑80/+69) | rpsO/truB | 30S ribosomal subunit protein S15/tRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base A (56/36); new base T (3/2); total (59/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAT > minE/2136788‑2136926 | cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTa > 1:310945/1‑70 (MQ=255) aaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAAc < 1:1924985/71‑1 (MQ=255) tatataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAt < 1:3145547/71‑1 (MQ=255) tatataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAt < 1:551907/71‑1 (MQ=255) atataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt > 1:571201/1‑71 (MQ=255) tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAttt < 1:3568197/71‑1 (MQ=255) tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt < 1:106420/70‑1 (MQ=255) tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt < 1:940605/70‑1 (MQ=255) aGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAc > 1:1300620/1‑70 (MQ=255) gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACTTTGcgt < 1:2934059/71‑2 (MQ=37) gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACTTTGcgt < 1:3008278/71‑2 (MQ=37) gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc < 1:1493192/71‑1 (MQ=255) gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg > 1:2141521/1‑70 (MQ=255) gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg < 1:846311/70‑1 (MQ=255) tGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGAc > 1:3107607/1‑71 (MQ=255) aaaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg > 1:548241/1‑65 (MQ=255) aaaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGt > 1:1814420/1‑71 (MQ=255) aaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGt > 1:1774086/1‑70 (MQ=255) aaGGACGACGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAAGCGACGt > 1:1913191/1‑70 (MQ=255) aGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:1065076/71‑1 (MQ=255) ggACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt > 1:1164704/1‑69 (MQ=255) gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc > 1:657847/1‑62 (MQ=255) gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc > 1:950517/1‑62 (MQ=255) gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGAc < 1:3672877/65‑1 (MQ=255) ctccGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACaa < 1:839327/51‑1 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:1358636/1‑70 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:3425046/1‑70 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:1365212/1‑70 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:824382/1‑70 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:734205/1‑70 (MQ=255) aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:700327/1‑70 (MQ=255) ctctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:649635/1‑68 (MQ=255) ctctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:649637/1‑68 (MQ=255) ctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGa > 1:112193/1‑53 (MQ=255) ctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTT‑AACAATTTACGCGACGTTAAGCGGCa < 1:3719325/64‑1 (MQ=255) tAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTAt < 1:3480607/68‑1 (MQ=255) aaTTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt > 1:2084192/1‑45 (MQ=21) aaTTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt > 1:3032617/1‑45 (MQ=21) aaTTCAGAGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt > 1:3027260/1‑45 (MQ=18) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:1744635/55‑1 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:2498458/55‑1 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:959648/55‑1 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:3653967/55‑1 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg > 1:1071269/1‑63 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa > 1:2389565/1‑65 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTc < 1:930057/68‑1 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCt > 1:2824995/1‑69 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTa > 1:1741347/1‑70 (MQ=255) aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTAc > 1:3707561/1‑71 (MQ=255) cAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTc < 1:1956606/70‑1 (MQ=255) cgCGATACCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGa > 1:474675/2‑46 (MQ=255) aGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg < 1:1026229/59‑1 (MQ=255) aGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGt < 1:3603841/71‑1 (MQ=255) gCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa > 1:1798180/1‑60 (MQ=255) gCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa > 1:1230292/1‑60 (MQ=255) gATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg < 1:223756/56‑1 (MQ=255) aTCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa < 1:335425/47‑1 (MQ=255) cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAg > 1:1191493/1‑47 (MQ=255) cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAg > 1:3623713/1‑47 (MQ=255) cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc > 1:1385670/1‑71 (MQ=255) ccaaGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc > 1:2125968/4‑71 (MQ=255) ccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACTTTAAGc < 1:482163/47‑1 (MQ=255) cAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg < 1:760908/51‑1 (MQ=255) gtgtACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGt > 1:3019671/1‑71 (MQ=255) gtgtACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGt > 1:2189302/1‑71 (MQ=255) tACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTAt > 1:2943063/1‑71 (MQ=255) aCGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATc > 1:1952580/1‑71 (MQ=255) cGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCg > 1:2446745/1‑71 (MQ=255) gTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCa < 1:1095974/42‑1 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt > 1:1724092/1‑43 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt > 1:2409155/1‑43 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt > 1:685018/1‑43 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt > 1:2885871/1‑43 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt > 1:598693/1‑43 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGc > 1:3712383/1‑70 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCa < 1:395454/71‑1 (MQ=255) ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGAGGCAGt > 1:2101742/1‑43 (MQ=255) tACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTa > 1:3399040/1‑54 (MQ=255) actcAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGc > 1:3602217/4‑68 (MQ=255) cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCt > 1:242419/1‑59 (MQ=255) cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCt > 1:2994281/1‑59 (MQ=255) gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGAc < 1:388077/71‑1 (MQ=255) gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGAc > 1:225774/1‑71 (MQ=255) aaaGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGa < 1:2341763/68‑1 (MQ=255) taatgtTAACCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCa < 1:2503040/61‑1 (MQ=255) aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACg > 1:2273553/1‑68 (MQ=255) aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGtt < 1:1024666/71‑1 (MQ=255) aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGtt < 1:2375306/71‑1 (MQ=255) gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCATGACGGTTa < 1:1245749/71‑1 (MQ=255) gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTa < 1:1276068/71‑1 (MQ=255) gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTa < 1:1965784/71‑1 (MQ=255) gtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCa < 1:1858545/60‑1 (MQ=255) ttAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACa > 1:1535568/1‑71 (MQ=255) ttAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACa > 1:2959410/1‑71 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc > 1:2895039/1‑46 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt < 1:1761972/70‑1 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt > 1:460365/1‑70 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt > 1:830768/1‑70 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt < 1:2057907/70‑1 (MQ=255) aaaCAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt > 1:2752866/1‑70 (MQ=255) | CAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAT > minE/2136788‑2136926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |