| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,184,929 | C→T | 9.4% | F213F (TTC→TTT) | gltD → | glutamate synthase, 4Fe‑4S protein, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,184,929 | 0 | C | T | 9.4% | 157.2 / 6.4 | 53 | F213F (TTC→TTT) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/26); new base T (2/3); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCC > minE/2184863‑2184999 | cTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAAc < 1:2523366/71‑1 (MQ=255)cTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAAc > 1:2475317/1‑71 (MQ=255)cTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAAc > 1:713448/1‑71 (MQ=255) tCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACt > 1:1891348/1‑70 (MQ=255) cAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTc > 1:2710120/1‑70 (MQ=255) cAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTc > 1:899802/1‑70 (MQ=255) cAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTc < 1:1614486/70‑1 (MQ=255) aaGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCaa < 1:2869729/71‑1 (MQ=255) aaGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCaa < 1:655852/71‑1 (MQ=255) ggAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATAcc > 1:152157/1‑70 (MQ=255) aaaaaGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGaa < 1:3436043/71‑1 (MQ=255) aaaaGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGCTATTGAATTCAAACTCAATACCGAAg > 1:91998/1‑71 (MQ=255) ggTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAGTTTGAATTCAAt < 1:642513/58‑1 (MQ=37) ggTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAGTTTGAATTCAAt < 1:2971377/58‑1 (MQ=37) ggTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAGTTTGAATTCAAt < 1:1393422/58‑1 (MQ=37) tAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAGTTTGAATTCAATAc > 1:1667051/1‑58 (MQ=25) tAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCcgc < 1:3543364/71‑1 (MQ=255) aTGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGa < 1:1393146/71‑1 (MQ=255) tGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGAc < 1:2744980/71‑1 (MQ=255) tGACGCGTCGCCGTGAAATCTTCACCGGCAT‑GGTATTGAATTCAAACTCTATACCGAAGTGGGCCGCGACg > 1:3632903/1‑71 (MQ=255) aCGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGt < 1:3713283/71‑1 (MQ=255) gcgTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTAc < 1:2208508/71‑1 (MQ=255) cgTCGCCGTTAAATCTTCACCGGCATGGGTATTGAATTCaa < 1:367648/41‑1 (MQ=255) cGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCt > 1:1743736/1‑71 (MQ=255) gTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGAc > 1:3074059/1‑71 (MQ=255) tGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCaa < 1:3134649/39‑1 (MQ=255) tGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACg < 1:2016355/71‑1 (MQ=255) aaaTCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAg > 1:2574364/1‑62 (MQ=255) aaaTCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGc > 1:3428586/1‑63 (MQ=255) cTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgc > 1:2816672/1‑71 (MQ=255) cTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgc > 1:2217924/1‑71 (MQ=255) cACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgc < 1:577511/68‑1 (MQ=255) aCCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAg < 1:3436563/71‑1 (MQ=255) cGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTgg < 1:1047854/56‑1 (MQ=255) gTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTa > 1:2737139/1‑41 (MQ=255) gTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTa > 1:1870738/1‑41 (MQ=255) gTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTa > 1:1239885/1‑41 (MQ=255) tATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATg > 1:2580327/1‑71 (MQ=255) tATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATg > 1:2435378/1‑71 (MQ=255) tttGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGa < 1:1691901/49‑1 (MQ=255) aTTGAGTTTGAATTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAg > 1:919780/1‑59 (MQ=37) gAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgc > 1:1953958/1‑52 (MQ=255) gAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGa > 1:3449711/1‑65 (MQ=255) aTTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTg < 1:971533/40‑1 (MQ=255) aTTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTg < 1:3680535/40‑1 (MQ=255) aTTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTg < 1:3495753/40‑1 (MQ=255) aTTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTg < 1:2645989/40‑1 (MQ=255) ttCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct < 1:3210376/50‑1 (MQ=255) ttCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct < 1:3075954/50‑1 (MQ=255) ttCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct < 1:3008929/50‑1 (MQ=255) ttCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct < 1:2071862/50‑1 (MQ=255) ttCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct < 1:1196421/50‑1 (MQ=255) tCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTc > 1:2014867/1‑71 (MQ=255) cAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTcc > 1:3360243/1‑71 (MQ=255) | CTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCC > minE/2184863‑2184999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |