| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,216,701 | T→C | 19.3% | K638K (AAA→AAG) | yhdA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,216,701 | 0 | T | C | 19.3% | 126.1 / 22.9 | 57 | K638K (AAA→AAG) | yhdA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (22/24); new base C (5/6); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGC > minE/2216631‑2216765 | cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttt < 1:589400/71‑1 (MQ=255) cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt > 1:2483599/1‑71 (MQ=255) cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttctt < 1:2318608/71‑1 (MQ=255) aGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTc < 1:160478/71‑1 (MQ=255) gcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTCTTCACg > 1:2937501/1‑71 (MQ=255) cgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGt < 1:182253/71‑1 (MQ=255) cgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGt < 1:14258/71‑1 (MQ=255) cATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAttttt > 1:2116820/1‑63 (MQ=255) ttattCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGt < 1:499667/71‑1 (MQ=255) ttattCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGt < 1:1416848/71‑1 (MQ=255) tattCTACGTGAAAACGGATTAAACGTCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTa > 1:3217715/1‑71 (MQ=255) tattCTACGTGAAAACGGATTAAACGGCAGGTTACACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTa > 1:1192243/1‑71 (MQ=255) attCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTAt > 1:1292017/1‑71 (MQ=255) tACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTa > 1:7359/1‑63 (MQ=255) aCGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGt > 1:955775/1‑71 (MQ=255) tGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCaa < 1:1873634/66‑1 (MQ=255) aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:2565097/1‑71 (MQ=255) aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtg < 1:2132173/70‑1 (MQ=255) aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtg < 1:3707893/70‑1 (MQ=255) aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCt > 1:1647853/1‑69 (MQ=255) aaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:393569/1‑70 (MQ=255) aaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:1894603/1‑70 (MQ=255) aaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:861221/1‑70 (MQ=255) aCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAttttt < 1:1512080/46‑1 (MQ=255) aCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAttttt < 1:2916745/46‑1 (MQ=255) aCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGa < 1:768462/71‑1 (MQ=255) aCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGa < 1:1324626/71‑1 (MQ=255) ggATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:1844358/1‑67 (MQ=255) ggATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAgg < 1:381860/71‑1 (MQ=255) ggATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAg > 1:1112851/1‑70 (MQ=255) aTTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCt < 1:1090505/63‑1 (MQ=255) aTTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCt < 1:948790/63‑1 (MQ=255) tAAACGGCAGGTTAAACCTAGTATCTTTGTGAATATTTTTTCACGtt < 1:2846912/47‑1 (MQ=255) aaaCGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATc > 1:3390180/1‑52 (MQ=255) aaaCGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGAcgc > 1:436728/1‑70 (MQ=255) cGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTa > 1:2356592/1‑47 (MQ=255) ggTTAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGaaa < 1:505396/66‑1 (MQ=255) ggTTAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGaaa < 1:426917/66‑1 (MQ=255) ggTTAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGaaa < 1:2017481/66‑1 (MQ=255) gTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt < 1:154454/53‑1 (MQ=255) ttAAACCGAGTATCTTTGTGAATATTTTTTCACGt < 1:1773221/35‑1 (MQ=255) ttAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATc < 1:2347281/42‑1 (MQ=255) ttAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATc < 1:3714512/42‑1 (MQ=255) tAAACGGAGTATGTTTGTGAATATTTCTTCACGTTAGTATc < 1:2844600/41‑1 (MQ=37) tAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATccc > 1:220161/1‑70 (MQ=255) tAAACCGAGTATCTTTGTGAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATcccc > 1:695361/1‑71 (MQ=255) aCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGa > 1:3274316/1‑50 (MQ=255) aCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGa > 1:3160564/1‑50 (MQ=255) cGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATccc > 1:2110758/1‑65 (MQ=255) gAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGAcgc > 1:2775059/1‑53 (MQ=255) gAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGaaa < 1:3730738/57‑1 (MQ=255) gAGTATCTTTGTGAATATATCTTCACGTTAGTATCAAGTg > 1:2643433/1‑40 (MQ=255) aGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCg < 1:283122/71‑1 (MQ=255) tCTTTGTGAATATTTTTTCACGTTTGTATCAAGTGGCtgt < 1:3453991/40‑1 (MQ=255) tCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt < 1:993776/40‑1 (MQ=255) tCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt < 1:583609/40‑1 (MQ=255) tCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt < 1:2858593/40‑1 (MQ=255) tCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt < 1:2666204/40‑1 (MQ=255) ttGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTaa > 1:1575047/1‑71 (MQ=255) tgtgAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGc > 1:1030320/1‑60 (MQ=255) gtgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGt < 1:1926791/67‑1 (MQ=255) gAATATTTCTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACAcc > 1:893498/1‑71 (MQ=255) atatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcgc > 1:83054/1‑71 (MQ=255) ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGaa < 1:2122631/39‑1 (MQ=255) ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGaa < 1:1553026/39‑1 (MQ=255) ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGaa < 1:1187509/39‑1 (MQ=255) | CGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGC > minE/2216631‑2216765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |