| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,249,100 | A→G | 5.1% | D97G (GAC→GGC) | thiF → | thiamin (thiazole moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,249,100 | 0 | A | G | 5.1% | 286.9 / 5.3 | 98 | D97G (GAC→GGC) | thiF | thiamin (thiazole moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (47/46); new base G (2/3); total (49/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TTACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGCCGAT > minE/2249031‑2249170 | ttACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGa > 1:310457/1‑70 (MQ=255)ttACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGa > 1:3728011/1‑70 (MQ=255) tACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACa > 1:1931028/1‑71 (MQ=255) tACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACa > 1:1533246/1‑71 (MQ=255) aCCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACa < 1:3486575/70‑1 (MQ=255) aCCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACAt > 1:2225614/1‑71 (MQ=255) ccACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACa < 1:377496/69‑1 (MQ=255) aaGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAAc < 1:2678762/69‑1 (MQ=255) aGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATGTCGGGATTCaa < 1:2955225/67‑1 (MQ=38) aGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATGTCGGGATTCaa < 1:1262891/67‑1 (MQ=38) aGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATGTCGGGATTCaa < 1:2776724/67‑1 (MQ=38) tatCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAg < 1:2109898/71‑1 (MQ=255) tatCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAg < 1:3253787/71‑1 (MQ=255) tatCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCAGACATTCAACTGACAg < 1:3601901/71‑1 (MQ=255) atcgatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAg > 1:2002520/1‑70 (MQ=255) tcgatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCa < 1:1193030/61‑1 (MQ=255) tcgatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCa < 1:1612525/61‑1 (MQ=255) cgatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCAt > 1:1006459/1‑71 (MQ=255) cgatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCAGACATTCAACTGACAGCAt > 1:1439610/1‑71 (MQ=255) gatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAg < 1:511637/67‑1 (MQ=255) gatcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCAtt < 1:2838668/71‑1 (MQ=255) atcgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAg < 1:3313781/66‑1 (MQ=255) cgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCAtt > 1:1704172/1‑68 (MQ=255) cgCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCAt > 1:3391501/1‑67 (MQ=255) gAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACAtt > 1:1407061/1‑49 (MQ=255) gAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTacaa > 1:2209311/1‑67 (MQ=255) gAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTacaa > 1:1053957/1‑67 (MQ=255) gAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACaacaac > 1:2571391/1‑71 (MQ=255) gAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACaacaac > 1:59185/1‑71 (MQ=255) aaaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACg > 1:2066369/1‑71 (MQ=255) aaaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACg < 1:155787/71‑1 (MQ=255) aaaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACg < 1:2914453/71‑1 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:2633378/1‑71 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg < 1:2202934/71‑1 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:3552400/1‑71 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:3510594/1‑71 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:761964/1‑71 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:2568380/1‑71 (MQ=255) aaTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:2329542/1‑71 (MQ=255) aTCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATGTCGGGATTCAACt > 1:471242/1‑51 (MQ=21) tCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGtt < 1:221539/71‑1 (MQ=255) tCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGt > 1:3226309/1‑70 (MQ=255) cAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAAc < 1:2550780/71‑1 (MQ=255) aGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACg > 1:76835/1‑71 (MQ=255) ggTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACgg > 1:3213508/1‑71 (MQ=255) ggTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACgg > 1:1984309/1‑71 (MQ=255) gTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTAcaacaa < 1:1062202/60‑1 (MQ=255) gTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTAcaacaa < 1:2836354/60‑1 (MQ=255) gTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg < 1:1235658/63‑1 (MQ=255) tCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACTGTTAACGGGt < 1:1535744/71‑1 (MQ=255) tCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:3089535/71‑1 (MQ=255) tCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:259178/71‑1 (MQ=255) tCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:2972876/71‑1 (MQ=255) tCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:1666646/71‑1 (MQ=255) aGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGa < 1:1009428/71‑1 (MQ=255) gCCAACAGCGACTGACACAGTTGAATCCCGACATTCaa > 1:3092991/1‑38 (MQ=255) gCCAACAGCGACTGACACAGTTGAATCCCGACATTCaa > 1:3606443/1‑38 (MQ=255) gCCAACAGCGACTGACACAGTTGAATCCCGACATTCaa > 1:1781061/1‑38 (MQ=255) gCCAACAGCGACTGACACAGTTGAATCCCGACATTCaa > 1:2267382/1‑38 (MQ=255) gCCAACAGCGACTGACACAGTTGAATCCCGACATTCaa > 1:2376599/1‑38 (MQ=255) ccAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGa > 1:746357/1‑69 (MQ=255) aGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGtt > 1:3680797/1‑70 (MQ=255) aGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGt > 1:2039817/1‑69 (MQ=255) cGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaaa < 1:1750771/71‑1 (MQ=255) cGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaaa < 1:1236793/71‑1 (MQ=255) cGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaaa < 1:849242/71‑1 (MQ=255) cGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaa > 1:403044/1‑70 (MQ=255) gACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaaa < 1:469085/70‑1 (MQ=255) aCTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAg < 1:1206511/71‑1 (MQ=255) aCTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAg < 1:2266739/71‑1 (MQ=255) tGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:893385/56‑1 (MQ=255) tGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGt < 1:2833306/56‑1 (MQ=255) gACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGtt < 1:2544291/48‑1 (MQ=255) cacaGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCg < 1:3322120/71‑1 (MQ=255) cacaGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCg > 1:2780328/1‑71 (MQ=255) cacaGTTGAATCCCGACAGTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCg > 1:3370693/1‑71 (MQ=255) caGTTGAATCCCGACATTCAACTGACAGCATTACAACAACgg > 1:2991207/1‑42 (MQ=255) caGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGt > 1:1798135/1‑71 (MQ=255) caGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGt > 1:3383977/1‑71 (MQ=255) aGTTGAATGTCGGGATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt > 1:2863709/1‑71 (MQ=38) aGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAg > 1:3271254/1‑63 (MQ=255) aGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt < 1:1157989/71‑1 (MQ=255) aGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt < 1:1282952/71‑1 (MQ=255) ttGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGt > 1:2441946/1‑55 (MQ=255) ttGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGt > 1:2437659/1‑55 (MQ=255) gAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCa < 1:1188539/70‑1 (MQ=255) gAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCa < 1:2431431/70‑1 (MQ=255) aaTCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCAc < 1:671049/70‑1 (MQ=255) tCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTaaaa < 1:2404064/55‑1 (MQ=255) tCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCgg < 1:2973394/62‑1 (MQ=255) cccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACggg > 1:2650473/1‑70 (MQ=255) cccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGc > 1:3343496/1‑71 (MQ=255) ccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt > 1:374197/1‑62 (MQ=255) ccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt > 1:3087601/1‑62 (MQ=255) ccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGtt > 1:1560025/1‑62 (MQ=255) ccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGcc < 1:2241826/71‑1 (MQ=255) ccGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGcc > 1:862905/1‑71 (MQ=255) cGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCa < 1:302635/64‑1 (MQ=255) aCATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGCCGAt > 1:2870498/1‑71 (MQ=255) aCATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGCCGAt > 1:1423083/1‑71 (MQ=255) | TTACCACTGAAGATATCGATCGCCCGAAATCGCAGGTCAGCCAACAGCGACTGACACAGTTGAATCCCGACATTCAACTGACAGCATTACAACAACGGTTAACGGGTGAGGCGTTAAAAGATGCGGTTGCACGGGCCGAT > minE/2249031‑2249170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |