| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,417,841 | A→T | 7.4% | intergenic (‑115/+76) | yifK ← / ← rffM | predicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,417,841 | 0 | A | T | 7.4% | 154.3 / 4.1 | 54 | intergenic (‑115/+76) | yifK/rffM | predicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Reads supporting (aligned to +/- strand): ref base A (23/27); new base T (2/2); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
TCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAG > minE/2417771‑2417911 | tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaaa > 1:2558217/1‑71 (MQ=255) cGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt > 1:2950733/1‑71 (MQ=255) gTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt < 1:155786/70‑1 (MQ=255) gTTATGGGTACATCGACTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt < 1:2136598/70‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:2870946/71‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:2294767/71‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:1829701/71‑1 (MQ=255) aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:3121177/1‑69 (MQ=255) aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:1216331/1‑69 (MQ=255) aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:1360315/1‑69 (MQ=255) gggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:223658/1‑67 (MQ=255) ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAAt < 1:3418176/70‑1 (MQ=255) ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAAt < 1:2877529/70‑1 (MQ=255) gTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTCAAATTTTTAATGc < 1:148478/71‑1 (MQ=38) cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCtt < 1:1812322/70‑1 (MQ=255) aGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt < 1:1171846/55‑1 (MQ=255) aGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGta < 1:1937087/71‑1 (MQ=255) tgtTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:2610191/1‑55 (MQ=255) gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGtaataa > 1:3609455/1‑69 (MQ=255) gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa < 1:1693092/70‑1 (MQ=255) gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa < 1:2239170/70‑1 (MQ=255) aaaTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaaaaa < 1:3375993/71‑1 (MQ=255) aTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg > 1:1350343/1‑70 (MQ=255) aTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGc < 1:1945559/71‑1 (MQ=255) tGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaaa > 1:2897075/1‑43 (MQ=255) gTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg > 1:1763207/1‑68 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGa < 1:3521902/71‑1 (MQ=255) ttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAg > 1:3720192/1‑68 (MQ=255) tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:1611825/1‑41 (MQ=255) tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:708424/1‑41 (MQ=255) tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt > 1:1455356/1‑41 (MQ=255) tAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa < 1:3203790/57‑1 (MQ=255) aaTTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAg > 1:361706/1‑63 (MQ=255) tCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACg > 1:3537598/1‑70 (MQ=255) cAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAg > 1:1560230/1‑63 (MQ=255) aGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGC‑TTGTGTAATAAAAAAGCAGACAGGCGACGGAg < 1:3289395/70‑1 (MQ=255) ggAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGt > 1:2982409/1‑71 (MQ=255) ggAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGt > 1:2986715/1‑71 (MQ=255) gagaAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTg < 1:2029686/71‑1 (MQ=255) gaAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGAGACGGAGTGAc < 1:555097/71‑1 (MQ=255) aaaTGGCAAATAAAGCATTAAAAATTTGAATGCTTtg < 1:3485763/37‑1 (MQ=255) aaTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCa < 1:1086090/71‑1 (MQ=255) aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAg < 1:1537744/55‑1 (MQ=255) aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCAc < 1:2496896/71‑1 (MQ=255) aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCAc > 1:434099/1‑71 (MQ=255) cAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCg < 1:1950612/71‑1 (MQ=255) aaTAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaaa < 1:2914609/39‑1 (MQ=255) aaTAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc > 1:1382283/1‑71 (MQ=255) tAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGc < 1:3588175/49‑1 (MQ=255) aaGCATTCAAATTTTTAATGCTTTATTTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCt < 1:595707/70‑1 (MQ=25) aGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCttt < 1:1770973/71‑1 (MQ=255) gCATTCAAATTTTTAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc > 1:3718112/1‑65 (MQ=38) gCATTCAAATTTTTAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc > 1:1617364/1‑65 (MQ=38) gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGc > 1:842939/1‑45 (MQ=255) gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTa > 1:22606/1‑71 (MQ=255) gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTa > 1:2062843/1‑71 (MQ=255) aaaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACg > 1:2750471/1‑44 (MQ=255) aaaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACaaa < 1:1183090/70‑1 (MQ=255) aaaaTTTGAATGCTTTGTGTAATAAAAAAGCTGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAaga < 1:1633753/71‑1 (MQ=255) aaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACaaa > 1:598108/1‑69 (MQ=255) aaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAaga < 1:129030/71‑1 (MQ=255) aaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAaga < 1:176210/71‑1 (MQ=255) aTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAagagag > 1:527492/1‑71 (MQ=255) | TCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAG > minE/2417771‑2417911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |